Influence of Cold Exposure on Dopamine Content in Rat Brown Adipose Tissue

Mf, Blouquit; Gripois, D.; Roffi, J

doi:10.1055/s-2007-979143

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…Cold exposure activates tyrosine hydroxylase, producing a large increase in dopamine content and turnover rate in brown adipose tissue [60]. In contrast, activation of central nervous system dopamine DRD2 receptors inhibits sympathetically mediated interscapular brown adipose tissue thermogenesis in response to cold exposure, inducing hypothermia [61].…”

Section: Discussionmentioning

confidence: 99%

Effect of high-fat feeding on expression of genes controlling availability of dopamine in mouse hypothalamus

et al. 2010

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ObjectiveHypothalamic centers integrate external signals of nutrient availability and energy status and initiate responses to maintain homeostasis. Quantifying changes in hypothalamic gene expression in the presence of nutrient excess may identify novel responsive elements.MethodsAffymetrix Mouse Genome 430 2.0 oligonucleotide microarrays containing 45 102 probe sets were used to interrogate differential expression of genes in dietary-induced obesity model C57BL6 inbred mice fed a high-fat (35% fat; n = 8) or standard (4% fat; n = 6) diet from 3 to 15 wk of age. Ontologies of regulated genes were examined and expression of selected genes was validated by quantitative real-time polymerase chain reaction.ResultsOne thousand two hundred twelve unique gene transcripts showed altered expression on the microarrays. Gene ontology analysis revealed changes in neuropeptide genes responding to leptin, Pomc, Cart, Npy, and Agrp, compatible with a homeostatic response to high-fat intake, although mean weight increased 2.3-fold compared with standard fed mice (P < 0.001). Neurotransmitter system ontologies revealed upregulation of five genes controlling availability of dopamine. Changes in Th tyrosine hydroxylase (2.1-fold) and Slc18a2 solute carrier family 18 (vesicular monoamine), member 2 (4.4-fold) controlling synthesis and release, and Slc6a3 solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (4.8-fold), Snca α-synuclein (1.3-fold), and Maoa monoamine oxidase (1.9-fold) limiting availability were confirmed by quantitative real-time polymerase chain reaction.ConclusionExpression of five genes involved in availability of dopamine was increased after a high-fat diet. Failure to reduce dopamine availability sufficiently, to counter the feeding reward effect, could contribute to diet-induced obesity in these mice.

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Section: Discussionmentioning

confidence: 99%

Effect of high-fat feeding on expression of genes controlling availability of dopamine in mouse hypothalamus

et al. 2010

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“…In a broader context, we provide comprehensive in vitro evidence for direct dopaminergic effects on brown adipose thermogenesis and mass. To our knowledge, only one study examined the effect of cold exposure, a very potent stimulus of thermogenesis, on catecholamine content of BAT in rats (Blouquit et al 1996). DA levels increased 4.7-fold on the first day and 17.9-fold on the 30th day of cold exposure, as compared to controls.…”

Section: Figurementioning

confidence: 99%

“…Our current study on dopaminergic effects on thermogenesis in vitro provides very plausible evidence for the physiological relevance of the in vivo data obtained from rats. Bloquit and coworkers expressed the catecholamine content of BAT as nanograms per fat pad; hence, the exact DA concentration in BAT remains unclear (Blouquit et al 1996). To address this question, we examined in a separate study the effects of cold exposure on BAT metabolism and plasma catecholamines in healthy males (Backhaus et al 2016).…”

Section: Figurementioning

confidence: 99%

“…Only few studies have thus far evaluated the contribution of DA to BAT thermogenesis in vivo. Cold exposure of rats, a strong stimulus of sympathetic nervous system (SNS) activity and BAT thermogenesis, causes an increase of DA concentrations in BAT (Blouquit et al 1996) and a rise of interscapular BAT temperature (Maxwell et al 1985b). Diet-induced thermogenesis also increases DA release from sympathetic nerves to BAT in rats (Rothwell et al 1982).…”

Section: Introductionmentioning

confidence: 99%

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Dopamine directly increases mitochondrial mass and thermogenesis in brown adipocytes

Kohlie

Perwitz

Resch

et al. 2017

Journal of Molecular Endocrinology

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Brown adipose tissue (BAT) is key to energy homeostasis. By virtue of its thermogenic potential, it may dissipate excessive energy, regulate body weight and increase insulin sensitivity. Catecholamines are critically involved in the regulation of BAT thermogenesis, yet research has focussed on the effects of noradrenaline and adrenaline. Some evidence suggests a role of dopamine (DA) in BAT thermogenesis, but the cellular mechanisms involved have not been addressed. We employed our extensively characterised murine brown adipocyte cells. D1-like and D2-like receptors were detectable at the protein level. Stimulation with DA caused an increase in cAMP concentrations. Oxygen consumption rates (OCR), mitochondrial membrane potential (Δψ) and uncoupling protein 1 (UCP1) levels increased after 24 h of treatment with either DA or a D1-like specific receptor agonist. A D1-like receptor antagonist abolished the DA-mediated effect on OCR, Δψ and UCP1. DA induced the release of fatty acids, which did not additionally alter DA-mediated increases of OCR. Mitochondrial mass (as determined by (i) CCCP- and oligomycin-mediated effects on OCR and (ii) immunoblot analysis of mitochondrial proteins) also increased within 24 h. This was accompanied by an increase in peroxisome proliferator-activated receptor gamma co-activator 1 alpha protein levels. Also, DA caused an increase in p38 MAPK phosphorylation and pharmacological inhibition of p38 MAPK abolished the DA-mediated effect on Δψ In summary, our study is the first to reveal direct D1-like receptor and p38 MAPK-mediated increases of thermogenesis and mitochondrial mass in brown adipocytes. These results expand our understanding of catecholaminergic effects on BAT thermogenesis.

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“…DA is not only important for neurological regulation but also for determination of other physiological processes such as brown adipose tissue (BAT) functioning: for example, cold exposure induces large increases in BAT as well as in DA concentrations. DA increase is probably due to stimulated activities of tyrosine hydroxylase activity observed under these conditions, while D-ß-H remains unchanged [10]. Thus, activation and inhibition of enzymes involved in DA synthesis and metabolisation seem to play a pivotal role as well in the regulation of behavioural as in metabolic determinations.…”

Section: Discussionmentioning

confidence: 94%

Biochemical Activities of Extracts from Hypericum perforatum L

Denke

Schempp

Weiser³

et al. 2011

Arzneimittelforschung

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Extracts from the herb "St. John's wort" (Hypericum perforatum L.) exhibit beneficial effects on patients suffering from mental depressions. Lack of catecholamine neurotransmitters may be one biochemical mechanism for this problem under discussion. It has been recently reported that alcoholic extracts from Hypericum perforatum inhibit dopamine-beta-hydroxylase (D-beta-H) with an I50 of 0.1 mumol/l on the basis of total hypericin content and with an I50 of 21 mumol/l with pure commercial hypericin. As test system polarographic determination of oxygen uptake with tyramine as a substrate analogue was used. In the present paper the quantification of the enzymatic activity and the potential influence of inhibitors are reported using dopamine as substrate and product (noradrenaline) quantification by HPLC. With this test system it could be shown that D-beta-H is strongly inhibited by pseudohypericin (I50 = approx. 3 mumol/l) and hypericin (I50 = approx. 5 mumol/l), whereas the I50-values of various flavonoids (quercitrin, isoquercitrin, hyperoside, rutin, quercetin, amentoflavone, kaempferol) are in the range of 50 mumol/l or higher.

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Influence of Cold Exposure on Dopamine Content in Rat Brown Adipose Tissue

Cited by 4 publications

References 11 publications

Effect of high-fat feeding on expression of genes controlling availability of dopamine in mouse hypothalamus

Effect of high-fat feeding on expression of genes controlling availability of dopamine in mouse hypothalamus

Dopamine directly increases mitochondrial mass and thermogenesis in brown adipocytes

Biochemical Activities of Extracts from Hypericum perforatum L

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