Influence of CO2 concentration and N:P ratio on Chlorella vulgaris-assisted nutrient bioremediation, CO2 biofixation and biomass production in a lagoon treatment plant

Molazadeh, Marzieh; Danesh, Shahnaz; Ahmadzadeh, Hossein; Pourianfar, Hamid R.

doi:10.1016/j.jtice.2019.01.005

Cited by 42 publications

(16 citation statements)

References 28 publications

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“…However, the developing of carbon capture and storage (CCS) or carbon capture and utilize (CCU) technology are two solutions for carbon dioxide emissions in large‐scale . The CCS technology can be divided into three steps: carbon capture, carbon transport, and carbon storage, which has received more and more attentions of most scientists …”

Section: Introductionmentioning

confidence: 99%

ARduino‐pH Tracker and screening platform for characterization of recombinant carbonic anhydrase in Escherichia coli

Hsu

Tan

Chiu

et al. 2019

Biotechnology Progress

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Carbonic anhydrase (CA, EC 4.2.1.1) is an ancient enzyme with zinc ion as its active site, which catalyzes the chemical reaction of carbon dioxide (CO2) to react with water and form bicarbonate ions. Due to its high catalytic efficiency on CO2 assimilation, CA is expected to use for carbon sequestration in industry. However, the protein expression level, thermostability and high‐throughput screening of an active CA are still with difficulty. In this study, the CA from Sulfurihydrogenibium yellowstonense (denoted as SyCA) was selected for overexpressed in Escherichia coli by different pET vectors. The enzymatic properties including thermo‐stability, pH tolerance, effect of metal ion, and kinetic parameters were characterized through a novel ARduino‐pH Tracker (ART) for monitoring online effectively. The SyCA is thermophilic and acidophilic as it maintains 100% activity at 50°C, while the residual activity is 34.8% after heating at 80°C for 150 min and the optimal pH is 3–5. The kinetic analysis by ART system showed that the k cat/K m of free enzyme was 4.4‐folds that that of whole cell. On the other hand, the screening platforms as Wilbur–Anderson unit, phenol red indicator and size of colony forming unit have been established to explore CA with higher activity. The high‐throughput screening platform is support in direct evolution of CA and further used in the industry.

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Section: Introductionmentioning

confidence: 99%

ARduino‐pH Tracker and screening platform for characterization of recombinant carbonic anhydrase in Escherichia coli

Hsu

Tan

Chiu

et al. 2019

Biotechnology Progress

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“…Molazadeh i in. In addition, [45] cultivated C. vulgaris in wastewater with compressed CO 2 at 16% and obtained 0.790 g•L -1 . An equally high biomass content (0.740 g•L -1 ) was obtained by Rodas-Gaitán et al [46], who cultured C. vulgaris in 15-L photobioreactors and used sodium bicarbonate at 8 g•L −1 as a carbon source.…”

Section: Biomass Production With Co 2 From Sodium Bicarbonatementioning

confidence: 99%

Utilisation of CO2 from Sodium Bicarbonate to Produce Chlorella vulgaris Biomass in Tubular Photobioreactors for Biofuel Purposes

2021

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Microalgae are one of the most promising sources of renewable substrates used for energy purposes. Biomass and components accumulated in their cells can be used to produce a wide range of biofuels, but the profitability of their production is still not at a sufficient level. Significant costs are generated, i.a., during the cultivation of microalgae, and are connected with providing suitable culture conditions. This study aims to evaluate the possibility of using sodium bicarbonate as an inexpensive alternative CO2 source in the culture of Chlorella vulgaris, promoting not only the increase of microalgae biomass production but also lipid accumulation. The study was carried out at technical scale using 100 L photobioreactors. Gravimetric and spectrophotometric methods were used to evaluate biomass growth. Lipid content was determined using a mixture of chloroform and methanol according to the Blight and Dyer method, while the carbon content and CO2 fixation rate were measured according to the Walkley and Black method. In batch culture, even a small addition of bicarbonate resulted in a significant (p ≤ 0.05) increase in the amount of biomass, productivity and optical density compared to non-bicarbonate cultures. At 2.0 g∙L–1, biomass content was 572 ± 4 mg·L−1, the maximum productivity was 7.0 ± 1.0 mg·L–1·d–1, and the optical density was 0.181 ± 0.00. There was also an increase in the lipid content (26 ± 4%) and the carbon content in the biomass (1322 ± 0.062 g∙dw–1), as well as a higher rate of carbon dioxide fixation (0.925 ± 0.073 g·L–1·d–1). The cultivation of microalgae in enlarged scale photobioreactors provides a significant technological challenge. The obtained results can be useful to evaluate the efficiency of biomass and valuable cellular components production in closed systems realized at industrial scale.

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“…Molazadeh et al [49] performed post-screening, biological treatment, and disinfection treatment of wastewater drained from sedimentation tanks of urban wastewater treatment plants. They controlled the N/P ratio and CO 2 concentration through the injection of potassium dihydrogen phosphate and used a high CO 2 concentration.…”

Section: The Ratio Of Nitrogen To Phosphorusmentioning

confidence: 99%

“…The analyses showed that Chlorella vulgaris demonstrated strong potential to remove phosphorus under all concentrations of CO 2 and N/P, exhibiting a removal range of 70.0-96.0%. Under conditions with 16% CO 2 and a ratio of 10:1 N:P, algae biomass was the highest, with an increase in lipid productivity, which makes a powerful contribution to the eventual recovery of microalgae for biofuel [49]. To summarize, the reduction of total phosphorus from urban wastewater by microalgae is not only related to the required N/P ratio but is also directly proportional to the biomass of the microalgae [48]; however, as microalgae process urban waste, the feedbacks between the N/P ratio, the concentration of CO 2 in the water body, and the regulation of the optimum concentration remain unclear [49].…”

Section: The Ratio Of Nitrogen To Phosphorusmentioning

confidence: 99%

Analysis of the Status and Improvement of Microalgal Phosphorus Removal from Municipal Wastewater

et al. 2021

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Phosphorus, as one of the main pollutants in municipal sewage, has received increasing attention recently. Phosphorus recovery also increases the sustainable development of municipal wastewater. Since algae have the ability to effectively redirect nutrients, including phosphorus, from municipal sewage to algae biomass, municipal sewage treatments involving microalgae have piqued the interest of many researchers. The phosphorus removal depends on the potential of the microalgae to absorb, preserve, or degrade phosphorus in municipal wastewater. It is, therefore, of great interest to study the mechanisms underlying the absorption, storage, and degradation of phosphorus by microalgae to ensure the viability of this phosphorus removal process in wastewater. The objectives of this review were to summarize phosphorus metabolism in microalgae, examine key external and internal factors impacting phosphorous removal by microalgae from wastewater, and examine the status of phosphorous-metabolism-related research to improve our understanding of microalgae-based municipal wastewater treatments. In addition, the methods of recovery of microalgae after phosphorous removal were summarized to ensure the sustainability of municipal wastewater treatment. Finally, a potential approach using nanomaterials was proposed to enhance the overall phosphorous removal performance in municipal wastewater through the addition of nanoparticles such as magnesium and iron.

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Influence of CO2 concentration and N:P ratio on Chlorella vulgaris-assisted nutrient bioremediation, CO2 biofixation and biomass production in a lagoon treatment plant

Cited by 42 publications

References 28 publications

ARduino‐pH Tracker and screening platform for characterization of recombinant carbonic anhydrase in Escherichia coli

ARduino‐pH Tracker and screening platform for characterization of recombinant carbonic anhydrase in Escherichia coli

Utilisation of CO2 from Sodium Bicarbonate to Produce Chlorella vulgaris Biomass in Tubular Photobioreactors for Biofuel Purposes

Analysis of the Status and Improvement of Microalgal Phosphorus Removal from Municipal Wastewater

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