Influence of citrate and EDTA anticoagulants on plasma malondialdehyde concentrations estimated by high-performance liquid chromatography

Suttnar, Jiřı́; L, Másová; Dyr, Jan E.

doi:10.1016/s0378-4347(00)00453-9

Cited by 71 publications

(42 citation statements)

References 14 publications

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“…MDA is one of the main products of lipid oxidation and it has been widely used as an index of lipid peroxidation (Suttnar et al, 2001). The current investigation showed a relation between osteoporosis and ROS generation, since the OVX rats showed a significant elevation of MDA level and significant decrease of GPx, SOD and TAC activities.…”

Section: Discussionmentioning

confidence: 99%

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

Sayed¹,

Soliman²,

Fahmy³

et al. 2013

Afr. J. Pharm. Pharmacol.

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The efficacy of Coelatura aegyptiaca shell powder (CES, 500 mg/kg BW) was evaluated as a calcium supplement in ovariectomized (OVX) rats for ten weeks of treatment. The biochemical components and the antioxidant properties of the shell powder were determined. The bone mineral density (BMD), bone mineral content (BMC), calcium (Ca) and phosphorus (P) contents in serum and bone, serum total alkaline phosphatase (TALP) and bone alkaline phosphatase (BALP), serum calcitonin and parathyroid (PTH) hormones were determined. Furthermore, some of the oxidative stress markers [malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant capacity (TAC)] were estimated in bone. The current study revealed that CES contained 19.38% Ca, 0.315% P as well as some of antioxidant amino acids which have a potent antioxidant activity against 1,1-diphenylpicrylhydrazyl (DPPH) free radical. Administration of CES to OVX rats increased BMD, BMC, tibial Ca and P contents and BALP activity, as compared to OVX rats. An ameliorative effect was recorded in the levels of calcitonin, PTH, MDA, SOD, GPx and TAC subsequent to CES administration to OVX rats.This ameliorative effect of CES powder against osteoporosis may be attributed to its antioxidant efficacy and/or to its Ca content. In conclusion, CES may have the potential to develop a clinically useful anti-osteoporotic agent, since its effect was comparable with alendronate (6.5 mg/kg BW/week).

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Section: Discussionmentioning

confidence: 99%

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

Sayed¹,

Soliman²,

Fahmy³

et al. 2013

Afr. J. Pharm. Pharmacol.

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“…14.4 mg of iron/L when added to human milk as intended. Commercially available ready-to-feed liquid formulas used for term infants included Enfamil With Iron (20,22,24 …”

Section: Human Milk Infant Formula Hmf Medicinal Iron Preparationsmentioning

confidence: 99%

Lactoferrin in the Preterm Infants' Diet Attenuates Iron-Induced Oxidation Products

et al. 2002

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“…Briefly, reaction mixtures for derivatization were prepared by adding 150 L of 0.44 M phosphoric acid, 5 L of 50 mM EDTA, 5 L of 2% butylated hydroxytoluene in ethanol, 10 -25 L of sample or standard (1,1,3,3-tetraethoxypropane), and 40% ethanol to a total volume of 185 L. For experiments in which r-Hu-Lf, desferrioxamine, and ferrous sulfate were added to formula or human milk, samples were incubated for 1 h at room temperature. Disodium EDTA and butylated hydroxytoluene were added to reaction mixtures to prevent artifactual formation of MDA during the derivatization procedure (22). After a 10-min room temperature incubation, 50 L of 0.6% thiobarbituric acid was added to form the thiobarbituric acid-MDA analyte (TBA 2 MDA).…”

Section: Iron Oxidative Stress and Lactoferrinmentioning

confidence: 99%

Lactoferrin in the Preterm Infants' Diet Attenuates Iron-Induced Oxidation Products

Raghuveer

2002

Pediatric Research

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Influence of citrate and EDTA anticoagulants on plasma malondialdehyde concentrations estimated by high-performance liquid chromatography

Cited by 71 publications

References 14 publications

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

Lactoferrin in the Preterm Infants' Diet Attenuates Iron-Induced Oxidation Products

Lactoferrin in the Preterm Infants' Diet Attenuates Iron-Induced Oxidation Products

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