Influence of chromium citrate on antioxidant defense in the liver of rats with experimentally induced diabetes

Sushko, O.; Iskra, R.; Pryimych, V. I.; Gzhytsky, Pekarska str Biotechnologies named after S. Z.

doi:10.15407/animbiol20.04.061

Cited by 2 publications

(3 citation statements)

References 18 publications

(11 reference statements)

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“…The activity of superoxide dismutase (SOD, ЕС 1.15.1.1.) was determined by the method described previously (Sushko et al, 2018) based on the enzyme's ability to compete with nitroblue tetrazolium for superoxide anion radicals, which are formed during the aerobic interaction of NADH and phenazine methosulfate. As a result of this reaction, nitroblue tetrazolium is reduced to formazan.…”

Section: Fig 2 Comparison Of Animal Groupsmentioning

confidence: 99%

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Antioxidant defense system of rat liver under the influence thiosulfonate esters

Liubas¹,

Iskra²,

Lubenets³

2023

Biol. Stud.

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Background. The article presents a study of the antioxidant defense system of the liver of rats under the influence of thiosulfonate esters: S-ethyl-4-aminobenzenethiosulfonate (ETS), S-allyl-4-aminobenzenethiosulfonate (ATS) and S-allyl-4-acetyl-aminobenzenethiosulfonate (AATS) at concentrations of 50 and 100 mg per kg of body weight. Thiosulfonate esters, which are synthetic sulfur-containing analogs of allicin, exhibit antioxidant and anti-inflammatory properties. The liver is the main organ where metabolism of xenobiotics and endogenous molecules occur to maintain metabolic homeostasis of the body, and is constantly exposed to reactive oxygen species (ROS) and subsequently to oxidative stress. Materials and Methods. The effectiveness of the antioxidant defense system in the rats’ liver was evaluated by measuring the level of oxidative stress markers (lipid peroxidation (LPO)) and the activity of the antioxidant enzymes – catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GP), glutathione reductase (GR), and the level of reduced glutathione (GSH). Results. The potential antioxidant properties of thiosulfonate esters and their dose-dependent effect in the liver were determined. In particular, under the action of thiosulfonate esters at a dose of 100 mg/kg, the content of lipid peroxidation products in the liver did not change significantly. Catalase activity and the content of reduced glutathione increased under the action of ETS dose of 100 mg/kg. Under the action of ATS and AATS doses of 100 mg/kg, the activity of GP decreased. At the same time, the effect of ETS at a dose of 50 mg/kg significantly decreased the level of lipid hydroperoxides. The effect of ATS and AATS doses of 50 mg/kg decreased TBA-reactive products. Under the action of the studied compounds in a lower dose, the activity of SOD and GP increased, and the content of reduced glutathione increased. At the same time, the decrease in the activity of GR under the action of AATS combined with an increased content of reduced glutathione is probably due to the inhibitory effect of the test substance on energy processes in the body. Conclusions. The studied thiosulfonate esters demonstrated a dose-dependent effect on the redox balance in the rats’ liver, proving to be more effective with lower doses of thiosulfonates.

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Section: Fig 2 Comparison Of Animal Groupsmentioning

confidence: 99%

“…Сatalase (CAT, EC 1.1.6) activity was estimated by the ability of H 2 O 2 to form a stable color formation with аmmonium molybdate salt. One unit of CAT activity was expressed as nmol of H 2 O 2 /min•mg of protein (Sushko et al, 2018).…”

Section: Fig 2 Comparison Of Animal Groupsmentioning

confidence: 99%

Antioxidant defense system of rat liver under the influence thiosulfonate esters

Liubas¹,

Iskra²,

Lubenets³

2023

Biol. Stud.

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“…The highest content of phospholipids, polyunsaturated fatty acids, Fe2 +, cations has been found in the brain. The most part of the oxygen being used by the body is spent on brain processes, which facilitates the development of lipid peroxidation reactions [3,5,18].…”

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Peroxidation Processes in the Rabbit Organism During Postnatal Ontogenesis

Rol

Tsekhmistrenko

Вовкогон

et al. 2020

Tehnologìâ virobnictva ì pererobki produktìv tvarinnictva

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One of the pressing problems of modern biochemistry is the problem of adaptation of animal organism to the environment and the formation of an adaptive reaction to the negative impact of production stress factors. Among such adaptive mechanisms for rabbits in the conditions of intensive rabbit meat management is the development of oxidative stress, which causes the accumulation of reactive oxygen species in the body and the development of reactive oxygen pathology. An important role in the mechanism of adaptation of the body belongs to lipids, because they are a structural component of cell membranes and act as energy and signal systems in cells. Peroxide oxidation of lipids is a compensatory reaction that ensures the functioning of the organism for changes in the environment. The content of total lipids and peroxide oxidation products of lipids, as well as the activity of enzymes of the antioxidant defense system in rabbits from birth to 90 days of age was investigated. It has been established that the content of total lipids in brain tissues increases throughout the period of postnatal ontogenesis due to the peculiarities of the functional and metabolic activity of brain cells. The content of common lipids is closely related to the processes of lipid peroxide oxidation and the activity of enzymes of antioxidant defense. The growth in concentration of peroxide oxidation products is accompanied by a decrease in the content of total lipids in the rabbit tissues. Reduced content of TBARSproducts in rabbit brain tissue from birth to 90-day age was noted. A moderate (r = 0.66) correlation between the content of lipid conjugated dienes and lipid hydroperoxides, as well as the strong correlation (r = -0.77) between the contents of lipid conjugated dienes and TBARS-products has been established. In the heart of rabbits a reversible moderate (r = -0.62) correlation between the content of lipid conjugated dienes and lipid hydroperoxides has been revealed. Key words: rabbits, development, lipid peroxidation, brain, heart, longest muscle of the back.

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Influence of chromium citrate on antioxidant defense in the liver of rats with experimentally induced diabetes

Cited by 2 publications

References 18 publications

Antioxidant defense system of rat liver under the influence thiosulfonate esters

Antioxidant defense system of rat liver under the influence thiosulfonate esters

Peroxidation Processes in the Rabbit Organism During Postnatal Ontogenesis

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