Influence of cholesterol on electroporation in lipid membranes of giant vesicles

Karal, Mohammad Abu Sayem; Ahamed, Md. Kabir; Mokta, Nadia Akter; Ahmed, Marzuk; Ahammed, Shareef

doi:10.1007/s00249-020-01443-y

Cited by 17 publications

(9 citation statements)

References 77 publications

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“…It plays important role in the functioning of real biological system, varying up to 50 mol% of the total lipid content. Particularly, the cholesterol inhibits the pore formation in the membranes and increases the line tension of membranes [27,28]. Lysenin (a pore forming toxin) induces pore formation in the lipid membranes in presence of cholesterol [29].…”

Section: Introductionmentioning

confidence: 99%

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles

et al. 2022

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The influence of cholesterol fraction in the membranes of giant unilamellar vesicles (GUVs) on their size distributions and bending moduli has been investigated. The membranes of GUVs were synthesized by a mixture of two elements: electrically neutral lipid 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol and also a mixture of three elements: electrically charged lipid 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), DOPC and cholesterol. The size distributions of GUVs have been presented by a set of histograms. The classical lognormal distribution is well fitted to the histograms, from where the average size of vesicle is obtained. The increase of cholesterol content in the membranes of GUVs increases the average size of vesicles in the population. Using the framework of Helmholtz free energy of the system, the theory developed by us is extended to explain the experimental results. The theory determines the influence of cholesterol on the bending modulus of membranes from the fitting of the proper histograms. The increase of cholesterol in GUVs increases both the average size of vesicles in population and the bending modulus of membranes.

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Section: Introductionmentioning

confidence: 99%

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles

et al. 2022

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“…With the addition of 0 to 40% chol in the membranes of DOPG/DOPC/chol-GUVs (surface charge density – 0.16 C m −2 ), the line tension increased from 12.9 to 14.6 pN. 79 This increase raises the value of U B ( r , σ n ), which decreases the rate of poration (see eqn (6) ). With the addition of cholesterol, the time of pore formation increases ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Effects of cholesterol on the anionic magnetite nanoparticle-induced deformation and poration of giant lipid vesicles

et al. 2022

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“…The researchers used cholesterol to prepare giant unilamellar vesicles, and found that the rupture of the plasma membrane under the electric field would be hindered by cholesterol. 37,[58][59][60] Most simulations suggested that cholesterol delayed the occurrence of electroporation, but the effect of cholesterol on the perforation propensity of lipid bilayer was strongly dependent on the structure of lipids. Mauroy et al reported that the decrease of poration propensity in POPC phospholipid was due to the increase content of cholesterol, 61 but Portet found that the increasing cholesterol accelerated the occurrence of electroporation in dioleoylphosphocholine (DOPC) vesicles.…”

Section: Introductionmentioning

confidence: 99%

All–atom molecular dynamics simulation of the combined effects of different phospholipids and cholesterol contents on electroporation

Guo

Wang

Zhou

2022

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The electroporation mechanism could be related to the composition of the plasma membrane, and the combined effect of different phospholipids molecules and cholesterol contents on electroporation is rarely studied and concluded. In this paper, we applied all-atom molecular dynamics (MD) simulation to study the effects of phospholipids and cholesterol contents on bilayer membrane electroporation. The palmitoyl-oleoyl-phosphatidylcholine (POPC) model, palmitoyl-oleoyl-phosphatidylethanolamine (POPE) model and 1:1 mixed model of the two (PEPC) were three basic models. An electric field of 0.45 V/nm was applied to nine models including three basic models with cholesterol contents of 0\%, 24\%, and 40\%. The interfacial water molecules moved under the electric field, and once the first water bridge formed, the rest of the water molecules would dramatically flood into the membrane. The simulation showed that a rapid rise in the Z component of the average dipole moment of interfacial water (Z-DM) indicated the occurrence of electroporation, and the same increment of Z-DM represented the similar change in the size of water bridge. With the same cholesterol content, the formation of the water bridge was the most rapid in POPC model regarding the average electroporation time ($t_{ep}$), and the average $t_{ep}$ of the PEPC model was close to that of the POPE model. We speculate that the difference in membrane thickness and initial hydrogen bonds of interfacial water affecting the average $t_{ep}$ among different membrane compostion. Our results reveal the influence of membrane composition on electroporation mechanism at the molecular level.

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Influence of cholesterol on electroporation in lipid membranes of giant vesicles

Cited by 17 publications

References 77 publications

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles

Effects of cholesterol on the anionic magnetite nanoparticle-induced deformation and poration of giant lipid vesicles

All–atom molecular dynamics simulation of the combined effects of different phospholipids and cholesterol contents on electroporation

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