Influence of chirality on catalytic generation of nitric oxide and platelet behavior on selenocystine immobilized TiO2 films

Abstract

As nitric oxide (NO) plays vital roles in the cardiovascular system, incorporating this molecule into cardiovascular stents is considered as an effective method. In the present study, selenocystine with different chirality (i.e., l- and d-selenocystine) was used as the catalytic molecule immobilized on TiO2 films for decomposing endogenous NO donor. The influences of surface chirality on NO release and platelet behavior were evaluated. Results show that although the amount of immobilized l-selenocystine on the surface was nearly the same as that of immobilized d-selenocystine, in vitro catalytic NO release tests showed that l-selenocystine immobilized surfaces were more capable of catalyzing the decomposition of S-nitrosoglutathione and thus generating more NO. Accordingly, l-selenocystine immobilized surfaces demonstrated significantly increased inhibiting effects on the platelet adhesion and activation, when compared to d-selenocystine immobilized ones. Measurement of the cGMP concentration of platelets further confirmed that surface chirality played an important role in regulating NO generation and platelet behaviors. Additionally, using bovine serum albumin and fibrinogen as model proteins, the protein adsorption determined with quartz crystal microbalance showed that the l-selenocystine immobilized surface enhanced protein adsorption. In conclusion, surface chirality significantly influences protein adsorption and NO release, which may have significant implications in the design of NO-generating cardiovascular stents.

A proteomics study to explore the role of adsorbed serum proteins for PC12 cell adhesion and growth on chitosan and collagen/chitosan surfaces

“…The results proved that the hydrophilic TiO 2 could significantly decrease Fib denaturation. Fan et al [ 61 ] used quartz crystal microbalance to determine the adsorption of bovine serum albumin (BSA) and Fib on the L- and D-selenocystine (L-Se/D-Se) immobilized TiO 2 films, and found that surface chirality led to different steric interactions between the negatively charged L-Se/D-Se surfaces and the negatively charged BSA and Fib, which in turn influenced hydrogen bonding and hydrophobic interaction, and eventually influenced protein adsorption. Svendsen et al [ 62 ] used ellipsometry to study the competitive adsorption of human serum albumin (HSA), human immunoglobulin G (IgG) and laminin-1 on the spin-coated and sintered hydroxyapatite (HA) surfaces.…”

Multifunctional Biodegradable Vascular PLLA Scaffold with Improved X-ray Opacity, Anti-Inflammation, and Re-Endothelization

“…However, Cys10 has significantly lower adhered platelets than Cys1. These results can prove that Cys10 has the ability to anti-platelet adhesion and activation [ 21 , 31 , 32 ].…”

Enzyme Mimics for the Catalytic Generation of Nitric Oxide from Endogenous Prodrugs

“…Wang et al were the first to report the activity of selenium compounds that decompose GSNO to generate NO in the presence of reducing agent GSH. [93] Based on this knowledge, several organoselenium species, including selenocystamine (SeCA), [94][95][96][97] selenocystine, [98] 3,3diselenodipropionic acid (SeDPA), [99][100][101] and 2,2'-diselenodiethanol (SeDO) [102] were studied by several groups to generate NO from RSNOs. The catalytic mechanisms of Se-contained enzyme mimics to decompose RSNOs to release NO in the presence of GSH are concluded as follows: [99] RSe Se-catalyzed reactions are highly selective towards RSNOs and exhibit no catalytic activity for nitrite or nitrate reduction.…”