Influence of chemotherapeutic agents on superoxide anion production by human polymorphonuclear leukocytes

Hara, Nobuyuki; Ichinose, Yukito; Motohiro, Akira; Kuda, Tomoharu; Aso, Hiroshi; Ohta, Mitsuo

doi:10.1002/1097-0142(19900815)66:4<684::aid-cncr2820660416>3.0.co;2-x

Cited by 21 publications

(12 citation statements)

References 13 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both polarization and decreased deformability are generally believed to be a consequence of actin filament polymerization associated with functional activation [13,30]. However, vindesine, unlike fMLP, did not cause functional activation, in agreement with previous reports that vindesine and vinblastine did not activate chemotaxis, 02-generation, or phagocytosis in neutrophils [32][33][34][35].…”

Section: Cytochalasln B Washsupporting

confidence: 87%

Transient neutropenia after intravenous injection of vindesine in patients with lung cancer

Aoshiba¹,

Nagai²,

Ueno³

et al. 1994

Eur J Clin Pharmacol

View full text Add to dashboard Cite

We have found transient circulating neutropenia and pulmonary sequestration of neutrophils after the intravenous injection of vindesine, a microtubule disruptor. Experiment 1 Ten patients with lung cancer were given a bolus intravenous injection of 3 mg.m-2 vindesine (Fildesine(r)). In all patients, total leukocyte and neutrophil counts in the venous blood fell to 65% and 47% of baseline values respectively within 30 min, and returned to baseline values within 6 h. In contrast, the lymphocyte count was stable. Experiment 2 Male Wistar rats were given saline or 0.08 mg.kg-1 vindesine intravenously and were sacrificed after 30 min. Vindesine produced a 58% reduction in the neutrophil count in the systemic circulation and a threefold increase in the neutrophil/erythrocyte ratio in the pulmonary microvasculature. Experiment 3 We studied the effects of vindesine in vitro on neutrophils and lymphocytes isolated from the venous blood of healthy volunteers. Vindesine (10(-5)-10(-8) mol.l-1) reduced neutrophil deformability (filterability) and induced neutrophil polarization, with reversibility of both effects after washout. These effects of vindesine were completely inhibited by cytochalasin B, an actin filament disrupter. Vindesine did not stimulate the neutrophil functions of adherence to polystyrene tubes, chemotaxis, or superoxide anion generation. The filterability and morphology of lymphocytes were not altered by vindesine. Thus, we conclude that a bolus injection of vindesine produces pulmonary sequestration of neutrophils, which produces circulatory neutropenia, and that it is primarily mediated by a decrease in neutrophil deformability that occurs without activation of the cells.

show abstract

Section: Cytochalasln B Washsupporting

confidence: 87%

Transient neutropenia after intravenous injection of vindesine in patients with lung cancer

Aoshiba¹,

Nagai²,

Ueno³

et al. 1994

Eur J Clin Pharmacol

View full text Add to dashboard Cite

show abstract

“…Bleomycin damages DNA by entering the cell nucleus and chelating with iron, thereby producing ROS. 29,31,32 Using this knowledge, immunostaining for 8OHdG was performed to measure damage. 33 The results revealed a high level of positive staining in the cell nuclei in the groups treated with bleomycin and ultrasound only and with ultrasound and MB combined.…”

Section: Discussionmentioning

confidence: 99%

“…Bleomycin was chelated with iron that was present in the cell, and reactive oxygen species (ROS) were refined as described. 29,30 When iron binds to the iron binding site within bleomycin it captures oxygen and produces a free radical (ROS) which induces an anti-tumor effect by cleaving DNA. 31,32 As a result of the ROS reaction, the production of the free radical 8OHdG within the cell nucleus was closely monitored.…”

Section: Evaluation Of Ultrasound/optison Anti-tumor Effectsmentioning

confidence: 99%

Inhibition of melanoma by ultrasound-microbubble-aided drug delivery suggests membrane permeabilization

Sonoda

Tachibana

Uchino

et al. 2007

Cancer Biology & Therapy

View full text Add to dashboard Cite

Ultrasound exposure-induced cavitation has been shown to accentuate cell membrane permeability, thus promoting effective drug delivery into cells, a technique that can be enhanced in the presence of microbubbles (MB). Here we applied this method as a treatment for malignant melanoma of the eyelid. The incidence of malignant melanoma in ophthalmology is relatively high, but its treatment is cosmetically difficult. A greater in vitro growth suppression of B-16 melanoma cells was achieved using ultrasound and MB in combination with the anticancer drug bleomycin than when a more concentrated dose of bleomycin alone was applied to the cell culture. Moreover, this effect was enhanced in an in vivo tumor model created by injecting B-16 melanoma cells into the lower eyelids of SCID mice. The antitumor effect of bleomycin was observed at a lower dose (0.5 mg/ ml) when the treatment was used in conjunction with ultrasound. The effect was further enhanced when MB were included, with tumor shrinkage occurring at bleomycin levels of 0.06 mg/ml. These results show that ultrasound and MB promote efficient bleomycin uptake by cells, and that the technique is a potentially useful drug delivery method.

show abstract

“…Hara et al (1990) showed that a number of chemotherapeutic agents, including cisplatin, suppressed the production of superoxide by human polymorphonuclear leukocytes. This suppression may arise from an effect on the cell membrane because the enzymes related to superoxide production are located on the surface of the cell membrane (Hara et al 1990). In addition, NADH oxidoreductase is the main source of superoxide anion in several tissues Griendling et ul.…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Cisplatin on the Production of NADH‐Dependent Superoxide and the Activity of Antioxidant Enzymes in Rat Renal Cortical Slices in vitro

Zhang

Lindup

1996

Pharmacology & Toxicology

View full text Add to dashboard Cite

Cisplatin-induced cytotoxicity has been investigated with rat renal cortical slices in vitro. Incubation of renal slices with cisplatin caused an unexpected decrease in NADH-dependent. lucigenin-enhanced chemiluminescence in the homogenate of the slices in a time- and concentration-dependent fashion. Cisplatin caused a concentration-related (0.2 1 mM) decrease in the formation of NADH-dependent superoxide anion. Cisplatin (2 mM) significantly suppressed the chemiluminescence to 70% of control as early as 15 min, and to 15% after 90 min. of incubation. A decrease was also observed 90 min. after incubation of slices with 0.25 mM cisplatin. In addition, the activities of superoxide dismutase (SOD) and catalase in the slices were significantly increased after 60 min. of exposure to cisplatin (2 mM) while decreases in the activities of glutathione (GSH) peroxidase and GSSG reductase became significant at 90 and 120 min. of incubation respectively. SOD and catalase activities were increased by 1.6 and 1.5 fold respectively after 90 min. of incubation and the activities of GSH peroxidase and GSSG reductase were decreased to 82% and 72% of control, respectively at 120 min. Both dithiothreitol (2 mM), a sulphydryl agent and diphenylphenylenediamine (5 microM), an antioxidant, protected against cisplatin-induced leakage of lactate dehydrogenase, lipid peroxidation and decreases of GSH peroxidase and GSSG reductase but had no effect on the decrease of chemiluminescence caused by cisplatin. The results suggest that neither an increase in the production of NADH-dependent superoxide anion nor a decrease in activity of several antioxidant enzymes were directly responsible for cisplatin-induced lipid peroxidation.

show abstract

Influence of chemotherapeutic agents on superoxide anion production by human polymorphonuclear leukocytes

Cited by 21 publications

References 13 publications

Transient neutropenia after intravenous injection of vindesine in patients with lung cancer

Transient neutropenia after intravenous injection of vindesine in patients with lung cancer

Inhibition of melanoma by ultrasound-microbubble-aided drug delivery suggests membrane permeabilization

Differential Effects of Cisplatin on the Production of NADH‐Dependent Superoxide and the Activity of Antioxidant Enzymes in Rat Renal Cortical Slices in vitro

Contact Info

Product

Resources

About