Influence of chelating agents on the toxicity and distribution of beryllium in rats

Abstract

The effect of two new chelating agents—Tiron (4,5‐dihydroxy‐1,3‐benzene disulphonic acid disodium salt) and succinic acid—on the mobilization of beryllium was studied. Animals were exposed to beryllium nitrate (1 mg kg−1 i.p.) daily for 21 days. Administration of beryllium nitrate showed a marked decrease in haemoglobin percentage, blood sugar, serum alkaline phosphatase and serum protein and a significant increase in the activity of transaminases. Tissue protein and glycogen contents and the activity of alkaline phosphatase, adenosine triphosphatase and succinic dehydrogenase showed significantly decreased values, but beryllium nitrate provoked a considerable increase in the activity of acid phosphatase and glucose‐6‐phosphatase in the vital and reproductive organs. Significant improvement in the haematological and biochemical parameters was observed with Tiron but no therapeutic effect was seen with succinic acid. Atomic absorption spectrophotometry (AAS) also showed a decreased level of beryllium concentration in the liver and kidney after Tiron therapy. © 1998 John Wiley & Sons, Ltd.

Study on the effects of nitrilotriproprionic acid and 4,5-dihydroxy-1,3-benzene disulphonate on the fractionation of beryllium in human serum using graphite furnace atomic absorption spectrometry

“…This change in the Be-HMW fraction is only significant at 10 -3 M. These observations confirm that Tiron complexes Be under serum conditions and increases the beryllium content in the HMW fraction by generating a mobilisation index that decreases with increasing Tiron concentrations (Figure 5 ). Tiron complexes Be in a 2:1 ratio [ 30 , 31 ], most probably generating a less charged complex that tends to be more soluble in the HMW fraction than in the hydrophilic LMW fraction. We suspect that such behaviour might increase the toxicity of beryllium, but several recent studies have shown that Tiron (with or without the aid of adjuvants) restored the altered biochemical physiological parameters and oxidative stress response in rats exposed to beryllium [ 15 , 18 , 31 ].…”

Protective effect of Tiron (4,5-dihydroxybenzene-1,3-disulfonic acid disodium salt) against beryllium-induced maternal and fetal toxicity in rats

“…Mathur et al (1993) have investigated the efficacy of three polyaminocarboxylic acids -ethylenediaminetetraacetic acid (CaNa 2 EDTA), diethylenetriamine pentaacetic acid (CaNa 3 DTPA) and N-(2-hydroxyethyl)-ethylenetriaminetriacetic acid (HEDTA) -against beryllium toxicity. Preliminary studies confirm the efficacy of Tiron, significantly over the other chelators Shukla et al 1998). …”

Atomic Spectrometry Update. Clinical and biological materials, foods and beverages

“…Sturup 28 described a method for the simultaneous determination of the four Zn isotopes and total Zn in human serum, urine and faeces by HR-ICP-MS. An RSD of 0.7% was determined for 67 Zn/ 66 Zn, 68 Zn/ 66 Zn and 70 Zn/ 66 Zn ratios and an RSD of 1.2% determined for the 64 Zn/ 66 Zn ratio. Using a mass resolution factor of 6000, all Zn isotopes were accurately determined, free from overlapping interferences, with the exception of 64 Ni on 64 Zn, which had to be corrected for mathematically.…”