Influence of chamber type integrated with computer-assisted semen analysis (CASA) system on the results of boar semen evaluation

Gączarzewicz, Dariusz

doi:10.1515/pjvs-2015-0106

Cited by 12 publications

(5 citation statements)

References 20 publications

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“…The above-mentioned researchers used a Leja chamber in their estimates, while a Makler chamber was used in the present study. Many researchers have stated that the CASA outcomes could be influenced by the analysis’ chambers [ 41 , 42 , 43 ]. In addition, in the present study, low-motility ejaculates were initially rejected because they could not be used for artificial insemination.…”

Section: Discussionmentioning

confidence: 99%

Effect of Boar Sperm Proteins and Quality Changes on Field Fertility

Michos

Tsantarliotou

Boscos

et al. 2021

Animals

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This study aimed to evaluate boar sperm characteristics and proteins, in relation to their importance regarding in vivo fertility. Sixty-five ejaculates were used and 468 sows (parity ≥ 2) were inseminated. Sperm CASA kinetics, morphology, viability, DNA fragmentation, mitochondrial membrane potential, sperm membrane biochemical activity (HOST) and sperm proteins (Heat Shock Protein 90-HSP90, glutathione peroxidase-5-GPX5, Osteopontin 70-OPN70) were assessed and related to field fertility (number of live-born piglets—NLBP, litter size ≥ 12 piglets—LS, farrowing rate—FR). Statistical analysis was conducted with simple and multiple regression models. Simple regression analysis showed that immotile sperm (IM) significantly affected the NLBP and LS, explaining 6.7% and 6.5% of their variation, respectively. The HOST positive spermatozoa significantly affected the NLBP and LS, explaining 24.5% and 7.8% of their variation, respectively. Similarly, sperm with activated mitochondria significantly affected the NLBP, explaining 13.5% of its variation. Moreover, the OPN70 affected LS and FR, explaining 7.5% and 10.8% of their variation, respectively. Sperm GPX5 protein affected FR, explaining 6.7% of its variation. Multiple regression analysis showed that the combination of IM and/OPN70 explains 13.0% of the variation regarding LS, and the combination of GPX5 and OPN70 explains 13.6% of the variation regarding FR. In conclusion, the estimation of parameters IM, membrane biochemical activity, mitochondrial membrane potential, OPN and GPX5 can provide useful information regarding semen doses for field fertility.

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Section: Discussionmentioning

confidence: 99%

Effect of Boar Sperm Proteins and Quality Changes on Field Fertility

Michos

Tsantarliotou

Boscos

et al. 2021

Animals

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“…The SCA system is based on the analysis of 25 consecutive digitized photographic images obtained from a single field under negative phase contrast and at 10× magnification. The parameters were adjusted to boar sperm, and the particle area was set at 10–80 μm [ 22 ]. Sperm with an average path rate (VAP) equal to or higher than 10 μm/s were classified as immotile.…”

Section: Methodsmentioning

confidence: 99%

Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests

Pezo,

Zambrano,

Uribe

et al. 2023

Animals

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The slow freezing of boar sperm is the only way to preserve genetic material for extended periods; this can be achieved with exposure to liquid nitrogen vapors (conventional) or by using automated freezing equipment. The aim was to compare the effect of both techniques on post-thaw functionality. Boar sperm devoid of seminal plasma and resuspended in lactose-egg yolk-glycerol medium were cryopreserved. Conventional: straws were exposed to LN2 vapors; automated: using a drop curve of −39.82 °C·min−1 for 113 s from −5 to −80 °C during the critical period; and subsequent immersion in NL2. Cell viability, cholesterol flow, mitochondrial membrane potential (MMP), lipid peroxidation, peroxynitrite, superoxide anion levels, phosphatidylserine translocation, and caspase activation were evaluated by flow cytometry. In addition, total motility (TM) and progressive motility (PM) were determined by the SCA system immediately (T0), 60 (T60), and 120 min (T120) post-thawing. Automated freezing significantly reduces cholesterol flow and free radical and lipid peroxidation levels, making it possible to preserve motility for 120 min of incubation. At the same time, viability, acrosome integrity, MMP, and caspase activation did not differ from the conventional technique. In conclusion, controlling the temperature drop curve using automated freezing equipment reduces oxidative/nitrosative stress, preserving membrane fluidity and sperm motility.

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“…However, one aspect that has been evaluated recently is the type of slide chamber used during the CASA assessment. In the study by Gączarzewicz (2015) three slide types were used: Leja chamber; Makler chamber; and a common glass microscope slide with a coverslip. The most important differences among these slides were the depths of the chamber in which the sperm were loaded and analyzed.…”

Section: Computer-assisted Sperm Analysismentioning

confidence: 99%

380 Factors Affecting Computer-Assisted Sperm Analysis (CASA) Measurements for Porcine Spermatozoa.

Zaritskiy

2018

Journal of Animal Science

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direction of Dr. William Flowers).Four experiments were conducted to determine the effects of time; dilution; slide location; and technician on common sperm quality estimates recorded using a computer-assisted sperm analysis (CASA) system. For each experiment, the split ejaculate technique was used with 18 boars being collected weekly for 5 to 6 weeks (n=100 to 130 observations per treatment per experiment). For analysis time, CASA measurements were obtained at 0, 30, 60, 90 and 120 s after loading the sample onto the evaluation chamber. Motility, progressive motility, curvilinear velocity (VCL), straight line velocity (VSL) and cell beat frequency (BCF) all decreased (p<0.05) over time with their highest measurements recorded at either 0 or 30 s. Amplitude of lateral head displacement (ALH) remained constant (p=0.23). For dilution, the neat sample was analyzed along with subsamples diluted with BTS extender in ratios of 1:1, 1:2, and 1:3.Increasing the dilution of neat semen had variable effects on CASA parameters. However, measurements for the 1:1 rate were consistently equal to or higher (p<0.05) than those recorded for neat semen and their counterparts with higher dilutions. For slide location, CASA measurements were recorded from 5 locations on the slide: lower central; middle central; upper central; middle right; and upper right. The location within the chamber at which measurements were taken had no effect on VCL (p=0.32); VSL (p=0.45); and BCF (p=0.14). For motility, progressive motility, and ALH all locations were similar (p>0.05) with the exception of the middle central in which the lowest (p<0.05) readings were recorded. For the effect of technician, 5 individuals with different levels of expertise with microscopes loaded and recorded CASA measurements from the same slide according to a randomized schedule. No differences (p>0.67) among technicians were observed for any of the CASA variables. Collectively, results from these studies indicate that neat samples should be diluted 1:1 and readings should be taken from any location except middle central slide area within 30 s of loading the sample in order to obtain the highest measurements. Prior experience of the technician performing CASA does not appear to have a significant effect on mobility estimates

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Influence of chamber type integrated with computer-assisted semen analysis (CASA) system on the results of boar semen evaluation

Cited by 12 publications

References 20 publications

Effect of Boar Sperm Proteins and Quality Changes on Field Fertility

Effect of Boar Sperm Proteins and Quality Changes on Field Fertility

Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests

380 Factors Affecting Computer-Assisted Sperm Analysis (CASA) Measurements for Porcine Spermatozoa.

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