Influence of cell adhesion and spreading on impedance characteristics of cell-based sensors

Asphahani, Fareid; Thein, Maung Maung; Veiseh, Omid; Edmondson, Dennis; Kosai, Ryan; Veiseh, Mandana; Xu, Jian; Zhang, Miqin

doi:10.1016/j.bios.2007.11.021

Cited by 83 publications

(62 citation statements)

References 26 publications

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“…These equivalent circuits have been used to model different types of cells on IMEs or other microelectrodes and for analysis of different impedance components (Ehret et al 1997;). Certainly, microelectrode-based systems for cell attachment could be modeled by more detailed and complex equivalent circuits (Asphahani et al 2008;Thein et al 2010). However, when the same IME-based system is used for different cell types, it is feasible to use the same equivalent circuit to interpret the impedance measurement results.…”

Section: Resultsmentioning

confidence: 99%

“…Our results here only showed a proof-of-concept that the IMEs impedance-based method has the potential to detect oral cancer cells and to distinguish them from noncancer oral epithelial cells. To scale this technique for real sample analysis, the use of well-designed microelectrodes that can perform single cell or a few cells impedance analysis Chen et al 2007;Asphahani et al 2008;Thein et al 2010), and the use of dielectrophoresis or other techniques to collect single cells or a few cells onto each electrode (Gray et al 2004) would be required. In addition, sample pre-treatment, such as depletion of the large number of lymphocyte and normal epithelial cells (Mauk et al 2007), would be needed.…”

Section: Cell Number (Cells) Maxmal Impedance Change At 24 H Measuredmentioning

confidence: 99%

“…In recent years, these impedance techniques have been gained increasing attention in the fields of cancer cell study and drug discovery (Solly et al 2004;Chen et al 2008;). Particularly, recent advances in microfabrication technology have made it possible to fabricate microdevices and microelectrodes for impedance study of a few cells and even a single cell Asphahani et al 2008;Thein et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Mamouni

Yang

2011

Biomed Microdevices

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In this study, electric/electrochemical impedance spectroscopy and cyclic voltammetry were used to study the cellular activities of oral cancer cell line CAL 27, including the kinetics of cell adhesion, spreading, and cell proliferation on interdigitated microelectrodes (IMEs). Impedance spectra of CAL 27 cells on IMEs electrodes were obtained in cell growth medium and in 0.1 M PBS with 50 mM [Fe(CN)₆]³⁻/⁴⁻ as redox probe. Equivalent circuits were used to model both cases. In cell growth medium, impedance spectra allowed us to analyze the changes in capacitance and resistance due to cell attachment on the IMEs over the entire experiment period. It was found that cell spreading caused the most significant decrease in capacitance component and slight increase in resistance component. Impedance change at given frequencies (between 10 kHz to 100 kHz) was found to be linearly increased with increasing cell number of CAL 27 on the IMEs. In comparison with non-cancer oral epithelial cells (Het-1A), at equal cell number, cancer cells always generated impedance several folds higher than that of non-cancer cells. In the presence of [Fe(CN)₆]³⁻/⁴⁻, impedance spectra allowed us to analyze the change in electron transfer resistance of IMEs due to cell attachment, in which an increase trend was observed at 24 h with increasing cell number from 2500 cells to 10,000 cells on IMEs. Double layer capacitance was also affected by cell attachment, and a decrease in double layer capacitance was observed with increasing cell number on the electrodes. Cyclic voltammetric measurements correlated well with the impedance results. The results of this study demonstrated the use of electrochemical approaches to obtain and understand cellular behaviors/activities of oral cancer cells, potentially providing useful tools for cancer cell research.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Number (Cells) Maxmal Impedance Change At 24 H Measuredmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Mamouni

Yang

2011

Biomed Microdevices

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“…Varshney and Li showed that the impedance change of Escherichia coli cells is due to the growth and concentration of the cells [17]. Asphahani et al and Panke et al indicated that the adhesion and spreading of cells significantly affect the impedance characteristics obtained using cell-based sensors [18,19]. Hong et al distinguished A549, MDA-MB-231, MCF-7, and HeLa cells using impedance spectra [20].…”

Section: Introductionmentioning

confidence: 97%

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

et al. 2014

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Impedance measurements provide basic electrical properties and are used to analyze the characteristics of electrochemical materials for biomedical applications. The extracellular fluid (ECF) in microfluidic devices greatly affects the accuracy of impedance measurements of cells. When a single cell is placed in large amounts of ECF, the electric current mostly passes through the ECF, not the cell. Hence, this work presents a modeling method that is demonstrated in numerical and analytical solutions for eliminating the effect of ECF in coplanar impedance sensors. The proposed modeling method uses fundamental formulas of circuits that include the electrical parameters of the ECF, cytoplasm, and cell membrane. Equivalent circuit models for the coplanar impedance sensor are established to simulate the impedance as well as the measured ones for excitation frequencies in the range of 11–101 kHz. According to the calculation result using the proposed modeling method, the cytoplasm resistance, membrane capacitance, medium resistance, and medium capacitance of HeLa (human cervix adenocarcinoma) cell are 13.5 kΩ, 122.6 pF, 27.9 kΩ, and 337.7 pF, respectively. Moreover, the electric current distribution in the coplanar impedance sensor is investigated using finite element method (FEM) simulation software. The variation in the impedance during measurements with the simultaneous application of an alternating‐current (AC) voltage amplitude of 0.4 Vpp in the fluid volume range of 9–144 µL is also studied.

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“…[1][2][3][4] The impedance value measured with different types of cells such as neural cells, endothelial cells, epithelial cells and cancer cells provides realtime monitoring of cell density, viability, and metabolic activity under a specific culture condition. [5][6][7][8] In real-time monitoring system, the impedance value is detected in direct technique using a pair of sensing and reference electrode submerged in cell culture medium.…”

Section: Introductionmentioning

confidence: 99%

Measurement of cell-substrate impedance and characterization of cancer cell growth kinetics with mathematical model

Park

Choi

Kim

2015

Int. J. Precis. Eng. Manuf.

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Non-invasive cell-substrate impedance sensing technique was used to monitor cancer cell attachment, spreading and proliferation as function of culture time. Impedance values were measured with cells cultured on collagen coated surface which exhibited moderate contact angle (54.5) and positively charge surface with the zeta potential (ζ) of +3.91 mV. Impedance was gradually increased and reached steady state after 2.6 hrs and 2.9 hrs with different impedance for SCC7 (2.7 Ω) and HeLa cells (1.1 Ω) respectively. Modified Gompertz mathematical growth model was used to determine growth kinetics, and showed maximum growth rate (µ m ) for SCC7 (7.9× 10 -4 hr -1 ) and HeLa cells (2×10 -5 hr -1 ) as well as both cells showed exponential increase of impedance following the lag period (λ) which required for cells to recover from the trypsinization and initiate attaching on electrode surface. The duration of lag period for SCC7 (0.7 hr) and HeLa cells (0.25 hrs ) demonstrated that HeLa cells exit lag period earlier than SCC7 cells and enter exponential growth which supports the rapid attachment and spread on substrate. Our result supported that the impedance measurement revealed cell-type specific growth characteristics and electrophysical properties that would be used to determine meaningful fingerprint for cancer cell study.

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Influence of cell adhesion and spreading on impedance characteristics of cell-based sensors

Cited by 83 publications

References 26 publications

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

Measurement of cell-substrate impedance and characterization of cancer cell growth kinetics with mathematical model

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