Influence of cation supplements on activity of netilmicin against Pseudomonas aeruginosa in vitro and in vivo

Barry, Arthur L.; Miller, George H.; Thornsberry, Clyde; Hare, R S; Jones, Ronald N.; Lorber, R R; Ferraresi, R. W.; Cramer, C A

doi:10.1128/aac.31.10.1514

Cited by 25 publications

(12 citation statements)

References 7 publications

(6 reference statements)

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“…It has been reported elsewhere that the MICs of aminoglycosides and tetracyclines increased upon addition of cations to the test media of P. aeruginosa [6,7]. In the current study, we showed that the addition of calcium elicited the same effect for aminoglycosides, tetracyclines, carbapenems, quinolones, and somelactams.…”

Section: Discussionsupporting

confidence: 81%

“…The minimum inhibitory concentrations (MICs) of aminoglycosides and tetracyclines increased when P. aeruginosa growth media was supplemented with cations [6,7]. To date, there have been no reports of similar increases in MICs for other antibiotics, and to the best of our knowledge, the relationship between resistance determinants and calcium concentrations has yet to be reported.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Calcium Concentrations on Antimicrobial Susceptibility Testing Against Pseudomonas aeruginosa

Yamaguchi¹,

Sato²,

Miura³

et al. 2014

J. Infect. Dis. Ther.

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Accurate determination of the antimicrobial susceptibilities of clinical isolates is important in establishing adequate treatment with antibiotics, particularly for infections caused by multiple drug-resistant strains of Pseudomonas aeruginosa. However, current laboratory antimicrobial susceptibility testing is affected by various conditions, including cation concentrations. In this study, we analyzed the relevance of the resistance determinants, metallo-lactamase (MBL) and aminoglycoside-modifying enzyme production, and examined the effects of calcium concentration on the in vitro activity of antibiotics. The broth microdilution method using Mueller-Hinton broth supplemented with various concentrations of calcium (5.2, 25, and 50 mg/L) was used to evaluate 58 clinical strains of P. aeruginosa. We observed increases in the minimum inhibitory concentrations (MICs) of aminoglycosides, tetracyclines, quinolones, and somelactam antibiotics with higher concentrations of calcium in the media. Strains that did not produce MBLs would have been affected by calcium concentration. Strains that did not produce aminoglycoside-modifying enzymes tended to lower the MICs of aminoglycosides more substantially than strains that produced aminoglycoside-modifying enzymes. In conclusion, the calcium concentration of the media may affect the results of antimicrobial susceptibilities of P. aeruginosa in relation to each resistance gene.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Effects of Calcium Concentrations on Antimicrobial Susceptibility Testing Against Pseudomonas aeruginosa

Yamaguchi¹,

Sato²,

Miura³

et al. 2014

J. Infect. Dis. Ther.

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“…Biofilm eradication was found different with the two media (Figure 1 and Figure 2). Different composition of media is reported to change the activity of antibiotics (27)(28)(29). The Ca 2+ and Mg 2+ ions in CaMHB media are required for a correct antimicrobial susceptibility testing because those ions reflect the divalent cation concentration in human blood (30)(31)(32)(33).…”

Section: Growth Mediamentioning

confidence: 99%

Influence of biofilm growth age, media and antibiotics exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro

Chen¹,

Xu²,

Winkler³

et al. 2020

Preprint

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Biofilm is known to be tolerant towards antibiotics and difficult to eradicate. Numerous studies have reported Minimum Biofilm Eradication Concentration (MBEC) values of antibiotics for many known biofilm pathogens. However, the experimental parameters applied in these studies differ considerably, and often the rationale behind the experimental design are not well described. This makes it difficult to compare the findings. To demonstrate the importance of experimental parameters, we investigated the influence of biofilm growth age, antibiotic treatment duration and growth media on biofilm eradication in this study. The commonly used biofilm model Calgary biofilm device was used to grow 24 h and 72 h biofilms of Staphylococcus aureus and Pseudomonas aeruginosa , which were treated with time-dependent vancomycin and concentration-dependent tobramycin, respectively. Two common bacteriological growth media Tryptic Soy Broth (TSB) and Cation-adjusted Mueller Hinton Broth (CaMHB) were tested. We found for both species that biofilms were more difficult to kill in TSB than in CaMHB. Furthermore, young biofilms (24 h) were easier to eradicate than old biofilms (72 h). In agreement with vancomycin being time-dependent, extension of the vancomycin exposure increased killing of S. aureus biofilms. Tobramycin treatment of 24 h P. aeruginosa biofilms was found concentration-dependent and time-independent, however, increasing killing was indicated for 72 h P. aeruginosa biofilms. This study demonstrated biofilm removal efficacy was influenced by media, biofilm age and antibiotics treatment duration. It is therefore necessary to taking these parameters into consideration when designing experiments.

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“…Bio lm eradication was found different with the two media (Figure 1 and Figure 2). Different composition of media is reported to change the activity of antibiotics (31)(32)(33). The Ca 2+ and Mg 2+ ions in CaMHB media are required for a correct antimicrobial susceptibility testing because those ions re ect the divalent cation concentration in human blood (34)(35)(36)(37).…”

Section: Growth Mediamentioning

confidence: 99%

Influence of biofilm growth age, media, antibiotic concentration and exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro

Chen

Thomsen

Winkler³

et al. 2020

Preprint

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Background: Biofilm is known to be tolerant towards antibiotics and difficult to eradicate. Numerous studies have reported minimum biofilm eradication concentration (MBEC) values of antibiotics for many known biofilm pathogens. However, the experimental parameters applied in these studies differ considerably, and often the rationale behind the experimental design are not well described. This makes it difficult to compare the findings. To demonstrate the importance of experimental parameters, we investigated the influence of biofilm growth age, antibiotic concentration and treatment duration, and growth media on biofilm eradication. Additionally, OSTEOmycinTM, a clinically used antibiotic containing allograft bone product, was tested for antibiofilm efficacy. Results: The commonly used Calgary biofilm device was used to grow 24 h and 72 h biofilms of Staphylococcus aureus and Pseudomonas aeruginosa, which were treated with time-dependent vancomycin (up to 3000 mg L-1) and concentration-dependent tobramycin (up to 80 mg L-1), respectively. Two common bacteriological growth media tryptic soy broth (TSB) and cation-adjusted Mueller Hinton broth (CaMHB) were tested. We found for both species that biofilms were more difficult to kill in TSB than in CaMHB. Furthermore, young biofilms (24 h) were easier to eradicate than old biofilms (72 h). In agreement with vancomycin being time-dependent, extension of the vancomycin exposure increased killing of S. aureus biofilms. Tobramycin treatment of 24 h P. aeruginosa biofilms was found concentration-dependent and time-independent, however, increasing killing was indicated for 72 h P. aeruginosa biofilms. Treatment with tobramycin containing OSTEOmycin TTM removed 72 h and 168 h P. aeruginosa biofilms after one day treatment, while few 72h S. aureus biofilms survived after two days treatment with vancomycin containing OSTEOmycin VTM. Conclusions: This study demonstrated biofilm removal efficacy was influenced by media, biofilm age and antibiotic concentration and treatment duration. It is therefore necessary to taking these parameters into consideration when designing experiments. The results of OSTEOmycinTM products indicated that simple in vitro biofilm test could be used for initial screening of antibiofilm products. For clinical application, a more clinically relevant biofilm model for the specific biofilm infection in question should be developed to guide the amount of antibiotics used for local antibiofilm treatment.

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Influence of cation supplements on activity of netilmicin against Pseudomonas aeruginosa in vitro and in vivo

Cited by 25 publications

References 7 publications

Effects of Calcium Concentrations on Antimicrobial Susceptibility Testing Against Pseudomonas aeruginosa

Effects of Calcium Concentrations on Antimicrobial Susceptibility Testing Against Pseudomonas aeruginosa

Influence of biofilm growth age, media and antibiotics exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro

Influence of biofilm growth age, media, antibiotic concentration and exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro

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