Influence of Catecholamines on Migration and Differentiation of GnRH Neurons in Rats

Izvol’skaya, M. S.; Voronova, S. N.; Макаренко, И. В.; Duittoz, Anne; Tillet, Yves; Kalas, A.; Ugrumov, M. V.

doi:10.1023/b:rudo.0000015120.17357.42

Cited by 4 publications

(7 citation statements)

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“…We propose that the catecholamine deficiency decreases, to the greatest extent, the rate of migration of GnRH-neurons in the area of their penetration in the brain. These data coincide with our earlier results (Izvolskaya et al, 2004). More caudally, in the septo-preoptic area, we did not find TH-immunoreactive neurons in the direct vicinity of GnRH-immunoreactive neurons, i.e., the "adult" pattern of innervation of the GnRH neurons by catecholaminergic fibers appears to be formed during early postnatal development.…”

Section: Discussionsupporting

confidence: 94%

“…The data on a decreased GnRH concentration in the caudal regions of migration of the GnRH-neurons also agree with our earlier results (Izvolskaya et al 2004). Although much more GnRH-neurons were detected in the septo-preoptic area, than in the anterior hypothalamus (Izvolskaya et al, 2004), the data presented in this paper suggest that the GnRH content in the anterior hypothalamus was higher than in the septo-preoptic area of intact animals (Fig.…”

Section: Discussionsupporting

confidence: 93%

“…Experimental data have recently been accumulated about the involvement of transmitters, such as γ -aminobutyric acid, glutamate, and serotonin, in the migration and differentiation of GnRH-neurons, as well in the regulation of GnRH-system already at the initial stages of its development (Adamskaya et al , 1998;Fueshko et al , 1998;Moore and Wray, 2000;Bless et al , 2000;Simonian and Herbison, 2001;Pronina et al , 2003a). We have already shown that the inhibition of catecholamine synthesis by the competitive inhibitor of the first enzyme of catecholamine synthesis tyrosine hydroxylase (TH) α -methyl-para -tyrosine ( α -MPT) delays the migration of GnRH-secreting neurons and changes the GnRH intracellular content (Izvolskaya et al , 2004).…”

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confidence: 95%

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Catecholamines in Regulation of Development of GnRH Neurons of Rat Fetuses

Izvol’skaya

Adamskaya²,

Voronova

et al. 2005

Russ J Dev Biol

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The contents of dopamine, serotonin, and noradrenaline in rat fetuses developing under the conditions of their deficiency induced by administration of α -methyl-para -tyrosine to females during 11th to 16th or 20th day of pregnancy and in fetuses, whose mothers were given saline at the same time, were determined using HPLC with subsequent electrochemical detection. Administration of α -methyl-para -tyrosine led to decreased levels of dopamine and noradrenaline in the areas of migration of GnRH-neurons in fetuses on days 17 and 21 of prenatal development. The concentration of serotonin remained unchanged, except in the head nasal area in males on day 21. The areas of interaction between the brain catecholaminergic systems and migrating and differentiating GnRH-neurons were determined by double immunohistochemical labeling. Close topographical location of GnRH-immunoreactive neurons and tyrosine hydroxylase-immunoreactive in the area of nucleus accumbens on days 17 and 20, as well as in the median eminence on day 20. The GnRH concentration in the caudal areas of migration of GnRH-neurons under the normal conditions and in the case of catecholamine deficiency was determined using radioimmunoassay. After administration of α -methyl-para -tyrosine the GnRH concentration in the anterior hypothalamus decreased in females. The data obtained suggest the involvement of catecholamines in the regulation of development of GnRH-Neurons during prenatal development. In addition, the adequacy and efficiency of the used model of catecholamine deficiency for studying the development of such neurons was confirmed.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

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confidence: 95%

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Catecholamines in Regulation of Development of GnRH Neurons of Rat Fetuses

Izvol’skaya

Adamskaya²,

Voronova

et al. 2005

Russ J Dev Biol

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“…It could be due to the increasing rate of proliferation or to the accumulation of ir product in migrating GnRH neurons (that made them detectable by ICC) after aMPT treatment. Indeed, GnRH-ir neurons were more intensively stained in the rostral areas of the migration pathway (Izvol'skaia et al 2004). From another hand, an excess of 5-HT and noradrenaline appears to inhibit the proliferation of the precursor cells of GnRH neurons (Pronina et al 2003b).…”

Section: Influence Of Cas On Gnrh Neuron Migrationmentioning

confidence: 99%

“…The GnRH neuron migration in foetuses from aMPT-treated mothers was compared to that in foetal rats from control mothers. Preliminary data were previously published in a Russian journal (Izvol'skaia et al 2004(Izvol'skaia et al , 2005. In this paper, special attention was paid to the topographic relationships between GnRH neurons and catecholaminergic fibres, using immunohistochemistry and confocal analysis.…”

Section: Introductionmentioning

confidence: 99%

The influence of catecholamine on the migration of gonadotropin-releasing hormone-producing neurons in the rat foetuses

et al. 2008

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Catecholamines (CA) play an important role in the regulation of GnRH neurons in adults, and it is probable that they control GnRH-neuron development. Migration of GnRH neurons was evaluated in male and female rats at the 17th embryonic day (E17) and E21, following the daily treatment of their pregnant mothers from the 11th to the 16th and 20th day of gestation with alpha-methyl-para-tyrosine (alphaMPT), an inhibitor of catecholamine synthesis. High-performance liquid chromatography with electrochemical detection (HPLC-ED) was used to specify the alphaMPT-induced CA depletion. There was a 50-70% decrease in dopamine and noradrenaline content in the nose and in the brain of alphaMPT-treated foetuses, proving the efficacy of this pharmacological model. Immunohistochemistry was used to evaluate the percentage (%) of GnRH neurons along their migration pathway from the vomeronasal organ (VNO) in the nose to the septo-preoptic area in the forebrain which is considered as an index of neuron migration. Special attention was paid to the topographic relationships of GnRH neurons with catecholaminergic fibres. These were observed in apposition with GnRH neurons in the entrance to the forebrain. In CA-deficient foetuses, the percentage of GnRH neurons located in the rostral regions extending from the VNO to the septum was greater than in controls. However, no statistically significant difference was found in the forebrain which extended from the septum to the retrochiasmatic area. In conclusion, these data suggest that endogenous catecholamines stimulate the GnRH neuron migration in ontogenesis.

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