Influence of carbon source on growth, biochemical composition and pigmentation of Ankistrodesmus convolutus

Chu, Wan-Loy; Phang, Siew‐Moi; Goh, S. H.

doi:10.1007/bf00003551

Cited by 27 publications

(13 citation statements)

References 13 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study, Scenedesmus obliquus showed a decrease in pigment concentration under mixotrophic conditions compared to autotrophic cultures. 45 Chu et al 46…”

Section: Resultsmentioning

confidence: 99%

Mixotrophic cultivation of Scenedesmus dimorphus in sugarcane bagasse hydrolysate

Manzoor

Ahmad

Aslam

et al. 2019

Env Prog and Sustain Energy

View full text Add to dashboard Cite

Overuse of the fossil fuels to fulfill existing energy requirements has generated various environmental problems like global warming. Emergence of environmental issues due to burning of the fossil fuel resources has provoked researchers to explore alternative sources of fuel. In this scenario, microalgal biofuels could present a promising alternative fuel if produced cost‐effectively without competing for freshwater resources and arable land. Aim of the present study was to grow microalgae by employing lignocellulosic waste for production of lipids. Scenedesmus dimorphus NT8c was chosen based on its ability to tolerate heat, rapid growth, and ease of harvesting by overnight settling. Biochemical composition and growth parameters of microalgae were analyzed when cultivated mixotrophically on sugarcane bagasse hydrolysate, a low‐value agricultural by‐product, that is, currently underutilized. Despite a slight increase in turbidity in the medium, S. dimorphus NT8c cultures raised mixotrophically in 5 g/L sugarcane bagasse hydrolysate displayed significantly higher growth rates compared to photoautotrophic cultivation with an overall biomass productivity of 119.5 mg L d−1, protein contents of 34.82% and fatty acid contents of 15.41%. Thus, microalgae cultivated mixotrophically are capable of photosynthesizing while metabolizing and assimilating organic carbon, significant increases of biomass and lipid productivity can be achieved. However, high supplementation with organic carbon can result in unfavorable levels of turbidity and bacterial growth, reducing microalgal biomass productivity.

show abstract

“…In another study, Scenedesmus obliquus showed a decrease in pigment concentration under mixotrophic conditions compared to autotrophic cultures. 45 Chu et al 46…”

Section: Resultsmentioning

confidence: 99%

Mixotrophic cultivation of Scenedesmus dimorphus in sugarcane bagasse hydrolysate

Manzoor

Ahmad

Aslam

et al. 2019

Env Prog and Sustain Energy

View full text Add to dashboard Cite

show abstract

“…To overcome this problem, more and more studies have involved the addition of doses of organic carbon into the growth medium to exploit the ability of microalgae to grow in a mixotrophic mode. Simultaneous increases in growth and lipid accumulation have been reported for a variety of carbon sources [ 8 – 12 ]. However, no studies have addressed the effects of organic carbon substrates on ARA accumulation in microalgae.…”

Section: Introductionmentioning

confidence: 99%

Using a trait-based approach to optimize mixotrophic growth of the red microalga Porphyridium purpureum towards fatty acid production

Jiao

Xiao

et al. 2018

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundOrganic carbon sources have been reported to simultaneously increase the growth and lipid accumulation in microalgae. However, there have been no studies of the mixotrophic growth of Porphyridium purpureum in organic carbon media. In this study, three organic carbon sources, glucose, sodium acetate, and glycerol were used as substrates for the mixotrophic growth of P. purpureum. Moreover, a novel trait-based approach combined with Generalized Additive Modeling was conducted to determine the dosage of each organic carbon source that optimized the concentration of cell biomass or fatty acid.ResultsA 0.50% (w/v) dosage of glucose was optimum for the enhancement of the cell growth of P. purpureum, whereas sodium acetate performed well in enhancing cell growth, arachidonic acid (ARA) and eicosapentaenoic acid (EPA) content, and glycerol was characterized by its best performance in promoting both cell growth and ARA/EPA ratio. The optimum dosages of sodium acetate and glycerol for the ARA concentration were 0.25% (w/v) and 0.38% (v/v), respectively. An ARA concentration of 211.47 mg L−1 was obtained at the optimum dosage of glycerol, which is the highest ever reported.ConclusionsThe results suggested that a comprehensive consider of several traits offers an effective strategy to select an optimum dosage for economic and safe microalgae cultivation. This study represents the first attempt of mixotrophic growth of P. purpureum and proved that both biomass and ARA accumulation could be enhanced under supplements of organic carbon sources, which brightens the commercial cultivation of microalgae for ARA production.

show abstract

“…To date, this is the first study involving the screening of the three microalgae for antiviral activity against EBV. Previous studies focused mainly on the screening of the microalgae for products such as fatty acids and pigments (Chu et al, 1995). The antiviral activities of the extracts were assessed based on their effects on the reduction of cell-free EBV DNA from the lymphoblastoid cells.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

Kok

Chu

Phang

et al. 2011

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

Abstract:This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against Epstein-Barr virus (EBV) in three Burkitt's lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC 50 <0.01 µg/ml) with a high therapeutic index (>28 000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC 50 =2.9 µg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA (EC 50 =1.38 µg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.

show abstract

Influence of carbon source on growth, biochemical composition and pigmentation of Ankistrodesmus convolutus

Cited by 27 publications

References 13 publications

Mixotrophic cultivation of Scenedesmus dimorphus in sugarcane bagasse hydrolysate

Mixotrophic cultivation of Scenedesmus dimorphus in sugarcane bagasse hydrolysate

Using a trait-based approach to optimize mixotrophic growth of the red microalga Porphyridium purpureum towards fatty acid production

Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

Contact Info

Product

Resources

About