Influence of carbon source on growth and mycotoxin production by isolates of Pyrenophora tritici-repentis from wheat

Bouras, Noureddine; Strelkov, Stephen E.

doi:10.1139/w10-073

Cited by 12 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(Table S16). Ptr is known to produce anthraquinone-derived phytotoxins (Bouras and Strelkov, 2010; Bouras et al 2009) and a biosynthetic cluster similar to the emodin cluster of Aspergillus nidulans (Bok et al 2009) was identified in the reference genome. Also found are clusters related to those in other fungi that produce resorcyclic-lactone-based compounds [ i.e.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics of a Plant-Pathogenic Fungus,Pyrenophora tritici-repentis, Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence

Manning

Pandelova

Dhillon

et al. 2013

G3 Genes|Genomes|Genetics

155

146

View full text Add to dashboard Cite

Pyrenophora tritici-repentis is a necrotrophic fungus causal to the disease tan spot of wheat, whose contribution to crop loss has increased significantly during the last few decades. Pathogenicity by this fungus is attributed to the production of host-selective toxins (HST), which are recognized by their host in a genotype-specific manner. To better understand the mechanisms that have led to the increase in disease incidence related to this pathogen, we sequenced the genomes of three P. tritici-repentis isolates. A pathogenic isolate that produces two known HSTs was used to assemble a reference nuclear genome of approximately 40 Mb composed of 11 chromosomes that encode 12,141 predicted genes. Comparison of the reference genome with those of a pathogenic isolate that produces a third HST, and a nonpathogenic isolate, showed the nonpathogen genome to be more diverged than those of the two pathogens. Examination of gene-coding regions has provided candidate pathogen-specific proteins and revealed gene families that may play a role in a necrotrophic lifestyle. Analysis of transposable elements suggests that their presence in the genome of pathogenic isolates contributes to the creation of novel genes, effector diversification, possible horizontal gene transfer events, identified copy number variation, and the first example of transduplication by DNA transposable elements in fungi. Overall, comparative analysis of these genomes provides evidence that pathogenicity in this species arose through an influx of transposable elements, which created a genetically flexible landscape that can easily respond to environmental changes.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Genomics of a Plant-Pathogenic Fungus,Pyrenophora tritici-repentis, Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence

Manning

Pandelova

Dhillon

et al. 2013

G3 Genes|Genomes|Genetics

155

146

View full text Add to dashboard Cite

show abstract

“…Analysis of the chromatograms for these 10 different cultivation media (Figure 1) clearly depicted a dramatic change in the chemical profiles. Cultivation on yeast extract glucose (GYE), 12 soya flour mannitol (SFM), 13 and potato dextrose (PD) 14 media resulted in the production of a sole metabolite, chaxamycin A (7), which was also found as a predominant metabolite in all of the other media used. As a result, the previously reported chaxamycin A (7) 6 is considered a useful chemical marker of this strain.…”

Section: ' Results and Discussionmentioning

confidence: 99%

Diverse Metabolic Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment

et al. 2011

View full text Add to dashboard Cite

The metabolic profile of Streptomyces sp. strain C34, isolated from the Chilean hyper-arid Atacama Desert soil, is dependent on the culture media used for its growth. The application of an OSMAC approach on this strain using a range of cultivation media resulted in the isolation and identification of three new compounds from the rare class of 22-membered macrolactone polyketides, named chaxalactins A-C (1-3). In addition, the known compounds deferroxamine E (4), hygromycin A (5), and 5″-dihydrohygromycin A (6) were detected. The isolated compounds were characterized by NMR spectroscopy and accurate mass spectrometric analysis. Compounds 1-3 displayed strong activity against Gram-positive but weak activity Gram-negative strains tested.

show abstract

“…While the case for such changes in morphology is unknown, it is possible that the reddish pigmentation observed in the colonies may be responsible for the red smudge symptoms, if in high abundance under the right conditions. Ptr is known to produce several mycotoxins from the anthraquinone class such as emodin, islandicin and catenarin [24][25][26], and both catenarin and emodin have been detected in Ptr-infected grain. Catenarin has been hypothesized to cause red smudge due to the red pigmentation property of the compound [27].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Pyrenophora tritici-repentis Infection of Wheat Heads

See¹,

Schultz²,

Moffat³

2020

Agriculture

View full text Add to dashboard Cite

The incidence of wheat head infection by Pyrenophora tritici-repentis (Ptr), the etiological agent of tan spot disease, was evaluated during grain development in a glasshouse experiment. Heads artificially inoculated with a Ptr spore suspension developed widespread brown spots across the spikelets, and mycelia and conidophores were observed on glumes and awns. Seeds of heavily infected heads were darkened and shrivelled, but no red smudge symptoms were apparent. The recovery rate of Ptr isolates from the inoculated wheat heads was low, and colonies that were re-isolated displayed an irregular morphology with reddish mycelia when grown on agar plates. The presence of Ptr on inoculated wheat heads was assessed directly via PCR detection, and a limitation of Ptr hyphae to proliferate beyond the point of contact of spore inoculum on floret tissues was observed. The systemic transmission of Ptr from infected seeds was minimal; however, saprophytic growth of the pathogen occurred on the senescing leaves of wheat plants grown from inoculated seeds. Thus, even though Ptr seed infection is not as common as foliar infection, infected seeds are still a source of disease inoculum and screening for pathogen contamination is advisable.

show abstract

Influence of carbon source on growth and mycotoxin production by isolates of Pyrenophora tritici-repentis from wheat

Cited by 12 publications

References 40 publications

Comparative Genomics of a Plant-Pathogenic Fungus,Pyrenophora tritici-repentis, Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence

Comparative Genomics of a Plant-Pathogenic Fungus,Pyrenophora tritici-repentis, Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence

Diverse Metabolic Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment

Evaluation of Pyrenophora tritici-repentis Infection of Wheat Heads

Contact Info

Product

Resources

About