Influence of caprine arthritis encephalitis on expression of ovulation related genes and activation of primordial follicles cultured in presence of phytohemagglutinin, epidermal growth factor or both

Advances in in vitro folliculogenesis in domestic ruminants

“…Within this culture system, several authors have reported the stimulation of follicle activation, and follicle and oocyte growth after a long-term culture (16 days) in the presence of: FSH during the first half (D0-D8) followed by either GH (Magalhães-Padilha et al, 2012a) or fibroblast growth factor-10 (FGF-10) during the second half (D8-D16) of the culture period (Almeida et al, 2015); KL during the first half and FSH during the second half of the culture (Lima et al, 2012); and also, FSH and IGF-I throughout the entire culture period, which turned up in increasing the percentage of secondary follicles (28%) . Other substances added for a shorter culture time (6 to 7 days) have also shown to exert a positive effect on follicle activation, survival and growth such as: the interaction between melatonin and FSH (Rocha et al, 2013); EGF (Lopes et al, 2015); KL (Faustino et al, 2013); and Concavalin A (Con A) (Portela et al, 2014). On the other hand, Keratinocyte growth factor-1 (KGF-1), also known as FGF-7, did not have a positive impact on early folliculogenesis in vitro (Faustino et al, 2013).…”