Influence of calcium phosphate crystal assemblies on the proliferation and osteogenic gene expression of rat bone marrow stromal cells

Liu, Yang; Cooper, Paul R.; Barralet, Jake E.; Shelton, Richard M.

doi:10.1016/j.biomaterials.2006.11.019

Cited by 121 publications

(89 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bone marrow stromal cells express lumican (23), suggesting that these cells could contribute to the observed phenotype. However, Lum Ϫ/Ϫ and Kera Ϫ/Ϫ mice reconstituted with wild-type bone marrow cells had impaired responses to LPS compared with wild-type recipients, indicating that the inhibition of neutrophil infiltration is the direct result of alteration of the extracellular matrix in the cornea.…”

Section: Discussionmentioning

confidence: 99%

Keratocan and Lumican Regulate Neutrophil Infiltration and Corneal Clarity in Lipopolysaccharide-induced Keratitis by Direct Interaction with CXCL1

Carlson

Lin

Liu

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

Reconstitution of keratocan and lumican expression in corneas of Kera؊/؊ and Lum ؊/؊ mice using adeno-keratocan and adeno-lumican viral vectors, respectively, resulted in normal neutrophil infiltration in response to LPS. Immunoprecipitation/Western blot analysis showed that lumican and keratocan core proteins bind the CXC chemokine KC during a corneal inflammatory response, indicating that corneal KSPGs mediate neutrophil recruitment to the cornea by regulating chemokine gradient formation. Together, these data support a significant role for lumican and keratocan in a corneal inflammatory response with respect to edema, corneal clarity, and cellular infiltration.

show abstract

Section: Discussionmentioning

confidence: 99%

Keratocan and Lumican Regulate Neutrophil Infiltration and Corneal Clarity in Lipopolysaccharide-induced Keratitis by Direct Interaction with CXCL1

Carlson

Lin

Liu

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…35 ALP is a marker for the early differentiation of osteoblasts; it regulates the inorganic phosphate metabolism by hydrolyzing phosphate esters and functions as a plasma membrane transporter for inorganic phosphates. 36 Alizarin-red staining is commonly used to detect the formation of calcium nodules (mineralization) -formed by osteoblasts in the late stage of differentiation -in osteoblast cultures. 37 In the present study, the MC3T3-E1 cells on the composite exhibited higher ALP activity and more calcium nodule-formation than PEEK and UHMWPE, indicating that the composite could promote the cell differentiation of MC3T3-E1 cells.…”

mentioning

confidence: 99%

Preparation, characterization, and in vitro osteoblast functions of a nano-hydroxyapatite/polyetheretherketone biocomposite as orthopedic implant material

Ma¹,

Tang²,

Tan³

et al. 2014

IJN

View full text Add to dashboard Cite

A bioactive composite was prepared by incorporating 40 wt% nano-hydroxyapatite (nHA) into polyetheretherketone (PEEK) through a process of compounding, injection, and molding. The mechanical and surface properties of the nHA/PEEK composite were characterized, and the in vitro osteoblast functions in the composite were investigated. The mechanical properties (elastic modulus and compressive strength) of the nHA/PEEK composite increased significantly, while the tensile strength decreased slightly as compared with PEEK. Further, the addition of nHA into PEEK increased the surface roughness and hydrophilicity of the nHA/PEEK composite. In cell tests, compared with PEEK and ultra-high-molecular-weight polyethylene, it was found that the nHA/PEEK composite could promote the functions of MC3T3-E1 cells, including cell attachment, spreading, proliferation, alkaline phosphatase activity, calcium nodule formation, and expression of osteogenic differentiation-related genes. Incorporation of nHA into PEEK greatly improved the bioperformance of PEEK. The nHA/PEEK composite might be a promising orthopedic implant material.

show abstract

“…Трансформация ОКФ в ГА -один из факторов, сти-мулирующих дифференциацию остеобластных клеток [3,9,14,16].…”

Section: экспериментально-теоретический разделunclassified

The impact of octacalcium phosphate on the dynamics of bone matrix formation in experimental bone defects

An¹,

et al. 2016

Stomat.

View full text Add to dashboard Cite

ЭКСПЕРИМЕНТАЛЬНО-ТЕОРЕТИЧЕСКИЙ РАЗДЕЛРазработка новых материалов для замещения кост-ных дефектов -одно из актуальных направлений хирур-гической стоматологии и имплантологии. Основная зада-ча заключается не в простом заполнении объема костного дефекта пластическим материалом, а в возможности ак-тивного включения последнего в активный жизненный цикл реципиентных тканей, в частности костных струк-тур.В настоящее время в литературе появилось большое число сообщений о разработке новых остеопластических материалов и эффективности их применения, сообщений часто поспешных и недостаточно обоснованных в плане репрезентативности отдаленных сроков исследований, что, несомненно, вносит хаос в представления об истин-ном состоянии дел, препятствует объективной оценке по-ложительных и отрицательных свойств предлагаемых и порой широко рекламируемых биоматериалов. В боль-шинстве своем все доступные на сегодняшний день син-тетические биоматериалы являются остеокондукторами, которые представляют собой биологически инертные ма-триксы. The aim of the study was to assess the interaction of of octacalcium phosphate (OCP) with bone matrix and cells and its impact on the process of bone generation. The survey was conducted on animal model: critical hipbone defect was created in 12 230-250 g Wister rats. The animals were then divided in two groups. In group 1 (6 animals) defect was left to heal under blood clot and in group 2 (6 animals) it was filled with OCP. Three animals with no defect served as a control group. It was showed significant (p<0.05) increase of the area of the newly formed bone tissue and its direct correlation with duration of observation. Keywords: bone, biomaterial, OCP, calcium phosphate, osteoinductivity. Биоинженерные конструкции (с использованием морфогенетических белков) дороги, иногда их цена в не-сколько раз превышает стоимость самой операции.Из образцов кальцийфосфатной керамики сегодня отдают предпочтение метастабильным формам. К ним можно отнести октакальцийфосфат (ОКФ), которому уделяется особое внимание как в нашей стране [1, 2,10,21], так и за рубежом [17,18].В тканевой среде ОКФ более растворим и менее ста-билен, чем синтетический гидроксиапатит (ГА). ОКФ об-ладает слоистой структурой из перемежающихся гидрати-рованных и апатитных слоев.Гидролиз ОКФ в ГА -процесс термодинамически выгодный, происходит спонтанно и необратимо. С этим связано то, что ОКФ является предшественником (мета-стабильной фазой) минерализации биологических апати-тов, так как он -центр кристаллизации минеральной фракции кости и зубов.Трансформация ОКФ в ГА -один из факторов, сти-мулирующих дифференциацию остеобластных клеток [3,9,14,16].

show abstract

Influence of calcium phosphate crystal assemblies on the proliferation and osteogenic gene expression of rat bone marrow stromal cells

Cited by 121 publications

References 55 publications

Keratocan and Lumican Regulate Neutrophil Infiltration and Corneal Clarity in Lipopolysaccharide-induced Keratitis by Direct Interaction with CXCL1

Keratocan and Lumican Regulate Neutrophil Infiltration and Corneal Clarity in Lipopolysaccharide-induced Keratitis by Direct Interaction with CXCL1

Preparation, characterization, and in vitro osteoblast functions of a nano-hydroxyapatite/polyetheretherketone biocomposite as orthopedic implant material

The impact of octacalcium phosphate on the dynamics of bone matrix formation in experimental bone defects

Contact Info

Product

Resources

About