Influence of brain gangliosides on the formation and properties of supported lipid bilayers

Jordan, Luke R.; Blauch, Megan E; Baxter, Ashley M.; Cawley, Jennie; Wittenberg, Nathan J.

doi:10.1016/j.colsurfb.2019.110442

Cited by 12 publications

(15 citation statements)

References 79 publications

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“…The vesicle rupturing is denoted by the rapid drop in the fluorescence signal of the single tracer vesicles, and the temporal change in the number of surface-adsorbed vesicles allows us to confirm the formation of a homogeneous SLB on the cover glass (Figure S3). A similar approach has been earlier reported by Mapar et al, Pace et al, Richards et al, and others to trace the formation of homogeneous SLB from a simple to a rather complex liposomal composition. − …”

Section: Resultsmentioning

confidence: 62%

SARS-CoV-2 Binding to Terminal Sialic Acid of Gangliosides Embedded in Lipid Membranes

et al. 2023

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Multiple recent reports indicate that the S protein of SARS-CoV-2 specifically interacts with membrane receptors and attachment factors other than ACE2. They likely have an active role in cellular attachment and entry of the virus. In this article, we examined the binding of SARS-CoV-2 particles to gangliosides embedded in supported lipid bilayers (SLBs), mimicking the cell membrane-like environment. We show that the virus specifically binds to sialylated (sialic acid (SIA)) gangliosides, i.e., GD1a, GM3, and GM1, as determined from the acquired single-particle fluorescence images using a time-lapse total internal reflection fluorescence (TIRF) microscope. The data of virus binding events, the apparent binding rate constant, and the maximum virus coverage on the ganglioside-rich SLBs show that the virus particles have a higher binding affinity toward the GD1a and GM3 compared to the GM1 ganglioside. Enzymatic hydrolysis of the SIA−Gal bond of the gangliosides confirms that the SIA sugar unit of GD1a and GM3 is essential for virus attachment to the SLBs and even the cell surface sialic acid is critical for the cellular attachment of the virus. The structural difference between GM3/GD1a and GM1 is the presence of SIA at the main or branched chain. We conclude that the number of SIA per ganglioside can weakly influence the initial binding rate of SARS-CoV-2 particles, whereas the terminal or more exposed SIA is critical for the virus binding to the gangliosides in SLBs.

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Section: Resultsmentioning

confidence: 62%

SARS-CoV-2 Binding to Terminal Sialic Acid of Gangliosides Embedded in Lipid Membranes

et al. 2023

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“…We expect vesicles with these compositions to significantly deform on SiO 2 surfaces because they will eventually rupture to form a SLB on SiO 2 . 35 Comparing the results in Figures 5 and 6 shows that the vesicle adsorption rate increases as a function of ganglioside mole fraction, but there is no significant difference in the vesicle deformation. The increase in adsorption rate can be explained by the higher probability of vesicle−MAG binding for each vesicle-surface encounter; however, once a vesicle is bound, the extent to which it is deformed is independent of the ganglioside mole fraction.…”

Section: ■ Results and Discussionmentioning

confidence: 90%

“…For comparison, the plots of Δ D vs Δ F for the adsorption of these vesicles on SiO 2 have significantly lower slopes (Figure ), which indicates that the vesicles deform significantly more on SiO 2 than they do on MAG-functionalized SLBs. We expect vesicles with these compositions to significantly deform on SiO 2 surfaces because they will eventually rupture to form a SLB on SiO 2 …”

Section: Resultsmentioning

confidence: 99%

Detection and Characterization of Vesicular Gangliosides Binding to Myelin-Associated Glycoprotein on Supported Lipid Bilayers

2020

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In the nervous system, a myelin sheath that originates from oligodendrocytes or Schwann cells wraps around axons to facilitate electrical signal transduction. The interface between an axon and myelin is maintained by a number of biomolecular interactions. Among the interactions are those between GD1a and GT1b gangliosides on the axon and myelin-associated glycoprotein (MAG) on myelin. Interestingly, these interactions can also inhibit neuronal outgrowth. Ganglioside–MAG interactions are often studied in cellular or animal models where their relative concentrations are not easily controlled or in assays where the gangliosides and MAG are not presented as part of fluid lipid bilayers. Here, we present an approach to characterize MAG–ganglioside interactions in real time, where MAG, GD1a, and GT1b contents are controlled and they are in their in vivo orientation within fluid lipid bilayers. Using a quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor functionalized with a supported lipid bilayer (SLB) and MAG, we detect vesicular GD1a and GT1b binding and determine the interaction kinetics as a function of vesicular ganglioside content. MAG-bound vesicles are deformed similarly, regardless of the ganglioside or its mole fraction. We further demonstrate how MAG–ganglioside interactions can be disrupted by antiganglioside antibodies that override MAG-based neuron growth inhibition.

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“…The plotted data contains the information required to calculate lateral diffusion coefficients, which are in turn used in membrane dynamics study. FRAP has been applied for comparative study of new types of liposomes [21][22][23], fluidity of the lipid bilayers [24,25] and proteins' dynamics in the membrane [26,27]. The main limitations of FRAP are photoswitching of analyte chemical or structural analytes and localised heating by the laser [28] which can change the sample.…”

Section: Fluorescence Of Membrane-bound Peptidesmentioning

confidence: 99%

Spectroscopy of model-membrane liposome-protein systems: complementarity of linear dichroism, circular dichroism, fluorescence and SERS

Rodger

2021

Emerging Topics in Life Sciences

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A range of membrane models have been developed to study components of cellular systems. Lipid vesicles or liposomes are one such artificial membrane model which mimics many properties of the biological system: they are lipid bilayers composed of one or more lipids to which other molecules can associate. Liposomes are thus ideal to study the roles of cellular lipids and their interactions with other membrane components to understand a wide range of cellular processes including membrane disruption, membrane transport and catalytic activity. Although liposomes are much simpler than cellular membranes, they are still challenging to study and a variety of complementary techniques are needed. In this review article, we consider several currently used analytical methods for spectroscopic measurements of unilamellar liposomes and their interaction with proteins and peptides. Among the variety of spectroscopic techniques seeing increasing application, we have chosen to discuss: fluorescence based techniques such as FRET (fluorescence resonance energy transfer) and FRAP (fluorescence recovery after photobleaching), that are used to identify localisation and dynamics of molecules in the membrane; circular dichroism (CD) and linear dichroism (LD) for conformational and orientation changes of proteins on membrane binding; and SERS (Surface Enhanced Raman Spectroscopy) as a rapidly developing ultrasensitive technique for site-selective molecular characterisation. The review contains brief theoretical basics of the listed techniques and recent examples of their successful applications for membrane studies.

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Influence of brain gangliosides on the formation and properties of supported lipid bilayers

Cited by 12 publications

References 79 publications

SARS-CoV-2 Binding to Terminal Sialic Acid of Gangliosides Embedded in Lipid Membranes

SARS-CoV-2 Binding to Terminal Sialic Acid of Gangliosides Embedded in Lipid Membranes

Detection and Characterization of Vesicular Gangliosides Binding to Myelin-Associated Glycoprotein on Supported Lipid Bilayers

Spectroscopy of model-membrane liposome-protein systems: complementarity of linear dichroism, circular dichroism, fluorescence and SERS

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