Influence of blanketing and season on vitamin D and parathyroid hormone, calcium, phosphorus, and magnesium concentrations in horses in New Zealand

Azarpeykan, Sara; Dittmer, Keren E.; Gee, Erica K.; Marshall, Jonathan; Wallace, John R.; Elder, Peter A.; Acke, Els; Thompson, K. G.

doi:10.1016/j.domaniend.2016.03.003

Cited by 29 publications

(39 citation statements)

References 33 publications

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“…Reports showed that most world's population are not getting sufficient amount of Vitamin D due to the current lifestyle and environmental factors that limit sunlight exposure. [20] Breast cancer research in the Middle East is extremely limited and genetics studies about cancer within Jordan are scarce except for few studies that screen gene polymorphism. [21222324]…”

Section: Discussionmentioning

confidence: 99%

Association between Serum 25-hydroxy Vitamin D Concentration and TaqI Vitamin D Receptor Gene Polymorphism among Jordanian Females with Breast Cancer

Atoum

Al-Khatib

2017

Chinese Medical Journal

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Background:Breast cancer is the most common type of cancer among females. Genetic polymorphisms might have a role in carcinogenesis. The aim of this study was to determine whether C to T base substitution within TaqI Vitamin D receptor (VDR) gene (rs731236) in exon 9 was a risk factor among patients with breast cancer.Methods:Peripheral blood was drawn from 122 Jordanian breast cancer patients and 100 healthy Jordanian volunteers in Al-Basheer Hospital during the summer months (from June to November of 2013, 2014, and 2015). DNA was amplified using polymerase chain reaction (PCR), followed by TaqI restriction enzyme digestion. Quantification of serum 25-hydroxy Vitamin D (25[OH]D) level was determined by competitive immunoassay Elecsys.Results:Genotypic frequencies for TaqI TT, Tt, and tt genotypes were 41%, 46%, and 13% for breast cancer compared to 42%, 50%, and 8% for control, respectively. Vitamin D serum level was significantly lower in the breast cancer patients (8.1 ± 0.3 ng/ml) compared to the control group (21.2 ± 0.6 ng/ml; P = 0.001). This study showed an inverse association between 25(OH)D serum level and breast cancer risk (odds ratio [OR], 22.72, 95% confidence interval [CI], 10.06–51.29).Conclusions:An inverse association was found between 25(OH)D serum level and breast cancer risk. Statistical difference was also found between different VDR TaqI genotypes and circulating levels of 25(OH)D among Jordanian females with breast cancer.

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Section: Discussionmentioning

confidence: 99%

Association between Serum 25-hydroxy Vitamin D Concentration and TaqI Vitamin D Receptor Gene Polymorphism among Jordanian Females with Breast Cancer

Atoum

Al-Khatib

2017

Chinese Medical Journal

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“…This result was unexpected given that skin coverage has a direct impact on 25(OH)D3 synthesis in other mammalian skin [122][123][124]. Interestingly however, serum 25(OH)D2 concentrations followed a seasonal pattern, decreasing in the winter months with the highest concentrations detected in the summer (again with no difference detected between blanketed and unblanketed animals) [88]. The same seasonal pattern was also determined for 25(OH)D2 measured in the grazing pasture consumed by the horses [88].…”

Section: Horsesmentioning

confidence: 90%

“…No reference range exists for 3-epi-25(OH)D3 in humans, therefore data from a systemic review which reported ranges from several studies was used (see Bailey et al (2013) [79]). The number of studies included to provide data for each of the veterinary species are as follows: (A) dogs n = 8 [80][81][82][83][84][85][86][87], cats n = 1 [85], horses n = 1 [88], sheep n = 4 [30,[89][90][91], cattle n = 2 [92,93], pigs n = 1 [94], poultry n = 2 [95,96] and nonhuman primates n = 2 [97,98]; (B) dogs n = 2 [81,85] and cats n = 1 [85]; and C. dogs n = 1 [83]. nd = no data available for the metabolites measured by LC-MS/MS in that species.…”

Section: Comparative Differences In Vitamin D Metabolismmentioning

confidence: 99%

“…Few studies have analyzed 25(OH)D2 and 25(OH)D3 in horse serum using a technique that can differentiate the two metabolites [110,112,113,120,121]. A recent study that evaluated serum 25(OH)D levels in horses using LC-MS/MS (which does distinguish between 25(OH)D2 and 25(OH)D3) demonstrated that 25(OH)D2 was the predominate 25(OH)D metabolite in horses [88]. 25(OH)D3 was undetectable above the lower limit of quantification (which was not specified) and there was no difference between horses that were blanketed or unblanketed for the study period of 13 months [88].…”

Section: Horsesmentioning

confidence: 99%

“…A recent study that evaluated serum 25(OH)D levels in horses using LC-MS/MS (which does distinguish between 25(OH)D2 and 25(OH)D3) demonstrated that 25(OH)D2 was the predominate 25(OH)D metabolite in horses [88]. 25(OH)D3 was undetectable above the lower limit of quantification (which was not specified) and there was no difference between horses that were blanketed or unblanketed for the study period of 13 months [88]. This result was unexpected given that skin coverage has a direct impact on 25(OH)D3 synthesis in other mammalian skin [122][123][124].…”

Section: Horsesmentioning

confidence: 99%

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Vitamin D Metabolism and Profiling in Veterinary Species

2020

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The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing other metabolites in the complex vitamin D pathway are being discovered in humans. Profiling of the vitamin D pathway by liquid chromatography tandem mass spectrometry (LC-MS/MS) facilitates simultaneous analysis of multiple metabolites in a single sample and over wide dynamic ranges, and this method is now considered the gold-standard for quantifying vitamin D metabolites. However, very few studies report using LC-MS/MS for the analysis of vitamin D metabolites in veterinary species. Given the complexity of the vitamin D pathway and the similarities in the roles of vitamin D in health and disease between humans and companion animals, there is a clear need to establish a comprehensive, reliable method for veterinary analysis that is comparable to that used in human clinical practice. In this review, we highlight the differences in vitamin D metabolism between veterinary species and the benefits of measuring vitamin D metabolites beyond 25(OH)D. Finally, we discuss the analytical challenges in profiling vitamin D in veterinary species with a focus on LC-MS/MS methods.

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Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol

Xiu

Sun

et al. 2022

Biotech & Bioengineering

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The synthesis of vitamin D3 precursor 7-dehydrocholesterol (7-DHC) by microbial fermentation has much attracted attention owing to its advantages of environmental protection. In this study, Saccharomyces cerevisiae was engineered for a de novo biosynthesis of 7-DHC. First, seven essential genes (six endogenous genes and one heterologous gene) were overexpressed, and the ROX1 gene (heme-dependent repressor of hypoxic genes) was knocked out. The resulting strain produced 82.6 mg/L 7-DHC from glucose. Then, we predicted five gene knockout targets for 7-DHC overproduction by the reconstruction of genome-scale metabolic model. GDH1 gene knockout increased the 7-DHC titer from 82.6 to 101.5 mg/L, and the specific growth rate of the ΔGDH1 mutant was also increased by 28%. Next, Ty1 transposon in S. cerevisiae was applied to increase the copies of the ERG1 gene and DHCR24 gene, resulting in a 120% increase in 7-DHC titer to 223.3 mg/L. Besides, to optimize the metabolic flux distribution, Clustered Regularly Interspaced Short Palindromic Repeats interference (CRISPRi) system was used to dynamically inhibit the competitive pathway, and the best binding site of ERG6 (delta (24)-sterol C-methyltransferase) promoter was screened out. The OD 600 value of ERG6 regulated cells increased by 43% than knocking out ERG6 directly, and 7-DHC titer increased to 365.5 mg/L in a shake flask. Finally, the 7-DHC titer reached 1328 mg/L in 3-L bioreactor and the specific titer of 7-DHC reached up to 114.7 mg/g dry cell weight). Overall, this study constructed a yeast chassis for the highly efficient production of 7-DHC by systems metabolic engineering.

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Influence of blanketing and season on vitamin D and parathyroid hormone, calcium, phosphorus, and magnesium concentrations in horses in New Zealand

Cited by 29 publications

References 33 publications

Association between Serum 25-hydroxy Vitamin D Concentration and TaqI Vitamin D Receptor Gene Polymorphism among Jordanian Females with Breast Cancer

Association between Serum 25-hydroxy Vitamin D Concentration and TaqI Vitamin D Receptor Gene Polymorphism among Jordanian Females with Breast Cancer

Vitamin D Metabolism and Profiling in Veterinary Species

Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol

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