Influence of Auxins on Glucosinolate Biosynthesis in Hairy Root Cultures of Broccoli (Brassica oleracea var. italica)

Abstract

Brassicaceae (Cruciferae) is one of the 10 most economically important plant families and includes vegetables such as broccoli, brussels sprouts, cabbage, cauliflower, kale, radish and various mustards. Among the cultivated Brassicaceae, broccoli attracted attention after it was discovered that it contains high levels of isothiocyanate sulforaphane. Isothiocyanate sulforaphane, [4-(methylsulfinyl)butyl isothiocyanate], is produced from the breakdown of glucoraphanin, [4-(methylsulfinyl) butyl GSL] and has great potential anticarcinogenic properties. Sulforaphane is the most potent naturally occurring Phase II enzyme inducer and is considered to play an important role in the detoxification of xenobiotic compounds when assimilated with diet 1,2 .Vegetables from the Brassicaceae family are among the most commonly grown vegetables worldwide. The family includes cabbage, broccoli, cauliflower, kale, collards and Brussels sprouts. Brassica vegetables are rich in bioactive compounds, including polyphenols, tocopherols, ascorbic acid, carotenoids and glucosinolates [3][4][5] . Epidemiological data have

Production of glucosinolates, phenolic compounds and associated gene expression profiles of hairy root cultures in turnip (Brassica rapa ssp. rapa)

“…We found that aromatic GSLs and IGSLs contents were prominent in hairy roots compared with that in non-transgenic roots. Consistent with our reports, gluconasturtiin, glucobrassicin, neoglucobrassicin, 4-hydroxyglucobrassicin, and 4-methoxyglucobrassicin were previously found to be leading in hairy roots of watercress (Park et al 2011) and broccoli (Kim et al 2013). Aromatic glucosinolate of glucotropaeolin was higher in hairy roots compared to callus, cell suspension culture and two-month-old plant leaves of Tropaeolum majus (Wielanek and Urbanek 1999).…”

Effect of Auxins on Anthocyanin Accumulation in Hairy Root Cultures of Tartary Buckwheat Cultivar Hokkai T10

“…Previously, we showed that lower Natural Product Communications Vol. 11 (9) 2016 1285 concentrations of IAA promoted the accumulation of different types of anthocyanins and glucosinolates in the HRCs of broccoli [27]; the findings of this study are consistent with those of our previous study. Anthocyanin content increased remarkably when 0.57 μM AA was used than when 2.85 μM was used.…”

Chemopreventive glucosinolate accumulation in various broccoli and collard tissues: Microfluidic-based targeted transcriptomics for by-product valorization

“…There has been some research aimed at producing glucosinolates using a hairy root culture system with broccoli and Arabidopsis [ 39 , 40 ]. A broccoli hairy root culture system with supplemented auxins has been shown to be more effective to produce indole glucosinolates compared Murashige and Skoog medium [ 41 ]. These methods may be a more feasible practice to utilize root tissue for bioactive glucosinolate production.…”