Influence of Atrazine and Roundup pesticides on biochemical and molecular aspects of Biomphalaria alexandrina snails

Barky, Fayez A.; Abdel-Salam, Hala Ali; Mahmoud, Momeana B.; Hamdi, Salwa

doi:10.1016/j.pestbp.2012.05.012

Cited by 46 publications

(25 citation statements)

References 51 publications

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“…Compared to Co − , both active compounds induced DNA alterations at all concentrations. Changes in the number, intensity, and position of the bands between the treated and untreated groups in ISSR and RAPD fingerprinting were related, caused by the herbicides atrazine and Roundup in Biomphalaria alexandrina snails (Barky et al 2012).…”

Section: Discussionmentioning

confidence: 95%

Toxicity of Difenoconazole and Tebuconazole in Allium cepa

Bernardes

Andrade-Vieira

Aragão

et al. 2015

Water Air Soil Pollut

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Macroscopic (germination and root growth) and microscopic (mitotic index, chromosome, and nuclear aberrations) analyses have been used to determine the toxicity of environmental pollutants. To better understand the molecular mechanisms of mutation and their effects, molecular markers offer a key perspective, as they measure the direct effects of DNA mutagenic agents. The aim of this study was to evaluate the toxic potential of the fungicides difenoconazole (DZ) and tebuconazole (TZ) on Allium cepa. A reduction was observed in the germination, root growth, and mitotic index at higher concentrations of DZ and TZ, compared to the negative control. In addition, high incidence of chromosome and nuclear aberrations was detected in treated roots. This demonstrates the genotoxic, cytotoxic, and phytotoxic effects of DZ and TZ on the root tips of A. cepa. Moreover, the molecular results indicate a change in the amplification profiles of the simple sequence repeats (SSR) and intersimple sequence repeats (ISSR) obtained from A. cepa after exposure to the tested compounds. Loss and gain of bands increased dose-dependently. Further, the grouping methods distinguished the higher concentrations from the negative control. The ISSR and SSR analyses proved to be efficient tools for evaluating DNA alterations caused by DZ and TZ. In association with macroscopic and microscopic analyses, they constitute an informative approach for environmental mutagen studies.

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Section: Discussionmentioning

confidence: 95%

Toxicity of Difenoconazole and Tebuconazole in Allium cepa

Bernardes

Andrade-Vieira

Aragão

et al. 2015

Water Air Soil Pollut

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“…This result is supported by Bakry (2009), Bakry, Abd el salam, Mahmoud, and Hamdi (2012), Bakry, El-Hommossany, and Ismil (2016), Bakry, Tantawy, Ragab, and Abdel Kader (2001), and Tantawy (2002). The reduction of egg production in treated snails could be attributed to their high mortality rates, different periods of ceasing oviposition during the experimental period, and deteriorations in the activities of antioxidant enzymes SOD, CAT, GR, peroxidation, TrxR, SDH, and fatty acid profile and protein patterns, which means a damage to the snails' cells, interrupting their physiological activities, suppressing their oviposition (Mx), and the reproductive rate (Ro) (El-Ansary, Sammour, Soliman, & Gawish, 2001;Ibrahim, 2006;Youssef, 2010).…”

Section: Discussionmentioning

confidence: 68%

Effect of mefloquine on biological and biochemical aspects of Lymnaea natalensis snails infected with Fasciola gigantica

Atwa

Bakry

2019

JoBAZ

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Background: The effect of the mefloquine drug on the survival rate, egg production, and infection of snails with Fasciola gigantica was studied. Also, the present study was designed to investigate the response of the snail Lymnaea natalensis for physiological and molecular aspects of the snail after exposure to mefloquine for 2 weeks. Results: It was found that the exposure of Lymnaea natalensis snails to mefloquine drug led to a significant reduction in the survival rate and egg production. The results obtained also showed that infectivity of Lymnaea natalensis with Fasciola gigantica miracidia was greatly reduced by exposure to LC25 of mefloquine drug. The data showed that in treating snails, glucose concentration (GL) in the haemolymph as well as lactate (LT) in soft tissues of treated snails increased, while glycogen (GN), pyruvate (PV), total protein (TP), and nucleic acids (DNA and RNA) levels in snail's tissues decreased. In addition, the activity level of some enzymes representing glycolytic enzymes as hexokinase (HK), pyruvate kinase (PK), phosphofructokinase (PFK), lactate dehydrogenase(LDH), and glucose phosphate isomerase (GPI) was also significantly reduced in response to treatment. Conclusion: DNA changes were studied by comet assay and the overall results revealed that mefloquine drug has genotoxic effect.

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“…These effects have been associated with increased susceptibility toward pathogen infection. Similar effects have been described for pesticides with different chemical nature, such as atrazine, hexachlorobenzene, glyphosate in snails , and lindane in sea urchins . However, experiments combining exposure to pesticides and subsequent bacteriological challenge have only been performed in the oyster Crassostrea gigas .…”

Section: Introductionmentioning

confidence: 72%

“…Cellular enzymes from different groups of organisms may be differentially affected by pesticide exposure. For example, high levels of acid phosphatase activity have been reported in the plasma of sprayers chronically exposed to mixed pesticides , whereas this enzyme's activity is negatively affected in the snail Biomphalaria alexandrina after atrazine and glyphosate exposure for 4 wk and remains unchanged in tissues of the freshwater fish Piaractus mesopotamicus during trichlorfon (organophosphorus) exposure. Activation of cellular acid phosphatase has been related to intensive lysosomal activity without leakage to plasma , which may be responding to immune stimulation and/or intracellular digestion of nutrients or foreign particles .…”

Section: Discussionmentioning

confidence: 99%

“…However, a negative modulation by AZM is observed only for alkaline phosphatase activity (decrease of activity in AZM + E. coli with respect to acetone + E. coli ). In this sense, Barky et al and Venturini et al suggested that pesticides may have a direct inhibitory effect, phosphorylating active sites (serine residues) of alkaline phosphatase, or may affect enzyme synthesis processes. According to this, it could be speculated that if AZM affected the phosphorylation of serine residues of enzymes such as phosphatases, its modulatory effect would be explained by changes in regulatory ways that involve serine/threonine protein kinases, such as protein kinase A and protein kinase C, among others.…”

Section: Discussionmentioning

confidence: 99%

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Modulation of immune and antioxidant responses by azinphos‐methyl in the freshwater mussel Diplodon chilensis challenged with Escherichia coli

Castro

Bianchi

Pascual

et al. 2016

Enviro Toxic and Chemistry

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The aim of the present study was to characterize the immune response-total hemocyte number, cell type proportion, hemocyte viability, lysosomal membrane stability, phagocytic activity, cellular acid and alkaline phosphatase activity, and humoral bacteriolytic and phenoloxidase activity--in Diplodon chilensis exposed to 0.2 mg/L of azinphos-methyl (AZM), using Escherichia coli as immunological and pro-oxidant challenges. In addition, glutathione-S-transferase and lipid peroxidation thiobarbituric acid reactive substances were analyzed in gill tissue. Mussels from an unpolluted site were treated for 3 d as follows: 1) experimental control; 2) solvent effects control (acetone 0.01%); 3) bacterial challenge effects control (E. coli, 5 cells/mL × 10 cells/mL); 4) pesticide effects control (AZM in acetone); 5) control for combined effects of solvent and bacterial challenge; and 6) exposed to AZM, then challenged with E. coli. The results showed increased granulocyte proportion and phagocytic activity. Partial reversion of deleterious effects of E. coli on lysosomal membranes was observed in mussels exposed to AZM and then challenged with E. coli. Total hemocyte number and humoral bacteriolytic activity were increased only by E. coli challenge. Acid phosphatase activity was increased by both E. coli and AZM, whereas the stimulating effect of E. coli on alkaline phosphatase activity was negatively modulated by AZM. Azinphos-methyl inhibited phenoloxidase activity regardless of the E. coli challenge. Gill glutathione-S-transferase activity was increased by E. coli treatment either alone or pretreated with acetone or AZM and by AZM alone. Thiobarbituric acid reactive substance levels were reduced by AZM alone or combined with the E. coli challenge and by acetone followed by the E. coli challenge. Both acetone and AZM seem to be important modulators of immune and antioxidant responses in D. chilensis. Environ Toxicol Chem 2017;36:1785-1794. © 2016 SETAC.

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Influence of Atrazine and Roundup pesticides on biochemical and molecular aspects of Biomphalaria alexandrina snails

Cited by 46 publications

References 51 publications

Toxicity of Difenoconazole and Tebuconazole in Allium cepa

Toxicity of Difenoconazole and Tebuconazole in Allium cepa

Effect of mefloquine on biological and biochemical aspects of Lymnaea natalensis snails infected with Fasciola gigantica

Modulation of immune and antioxidant responses by azinphos‐methyl in the freshwater mussel Diplodon chilensis challenged with Escherichia coli

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