Influence of Atmospheric Pressure Non-thermal Plasma on Inactivation of Biofilm Cells

Czapka, Tomasz; Maliszewska, Irena; Olesiak‐Bańska, Joanna

doi:10.1007/s11090-018-9925-z

Cited by 22 publications

(14 citation statements)

References 45 publications

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“…During the use of DBD plasma, the substrates were placed in an air gap between two electrodes, with an approx. 2 mm distance between the modified surface and the top electrode [ 40 , 41 ]. The process was conducted with a voltage of approx.…”

Section: Methodsmentioning

confidence: 99%

“…The observed changes in the geometry of the modified substrate surfaces can be a reflection of the dif ferent temperatures of the treatment processes in RF and DBD plasma. Modifications in volving DBD plasma are low-temperature processes [26,27,40], which do not cause signif icant changes in the geometric structure of the modified PDMS. In the case of modifica tions performed using RF PACVD equipment, the temperature of the modified surfac can exceed 100 °C [42].…”

Section: Surface Geometrical Structurementioning

confidence: 99%

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Impact of Plasma Pre-Treatment on the Tribological Properties of DLC Coatings on PDMS Substrates

et al. 2021

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The processes of the deposition of carbon coatings on PDMS (polydimethylsiloxane) substrates using plasma techniques are widely used in a large number of studies, in applications ranging from electronic to biological. That is why the potential improvement of their functional properties, including tribological properties, seems very interesting. This paper presents an analysis of the impact of plasma pre-treatment on the properties of the produced diamond-like carbon (DLC) coatings, including changes in the coefficients of friction and wear rates. The initial modification processes were performed using two different techniques based on low-pressure plasma (RF PACVD, radio-frequency plasma-assisted chemical vapour deposition) and dielectric barrier discharge (DBD) plasma. The effects of the above-mentioned treatments on the geometric structure of the PDMS surface and its water contact angles and stability over time were determined. The basic properties of the DLC coatings produced on unmodified substrates were compared to those of the coatings subjected to plasma pre-treatment. The most interesting effects in terms of tribological properties were achieved after the DBD process and production of DLC coatings, achieving a decrease in wear rates to 2.45 × 10−8 mm3/Nm. The tests demonstrate that the cross-linking of the polymer substrate occurs during plasma pre-treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Surface Geometrical Structurementioning

confidence: 99%

Impact of Plasma Pre-Treatment on the Tribological Properties of DLC Coatings on PDMS Substrates

et al. 2021

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“…Xu et al inactivated approximately 4 log 10 S. aureus biofilms after treatment for 10 min with a He/O 2 plasma jet, and Wang et al inactivated approximately 0.5 log 10 S. aureus cells in biofilms after 30 min of exposure to a N 2 plasma jet [ 19 , 20 ]. Czapka et al inactivated approximately 2.77 log 10 S. aureus biofilms after treatment with dielectric barrier discharge (DBD) for 300 s [ 21 ]. However, the molecular mechanism in the bacterial cells in biofilms inactivated by CAP is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric-Pressure Plasma Caused Protein Damage in Methicillin-Resistant Staphylococcus aureus Cells in Biofilms

Guo

Yang

et al. 2021

Microorganisms

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Biofilms formed by multidrug-resistant bacteria are a major cause of hospital-acquired infections. Cold atmospheric-pressure plasma (CAP) is attractive for sterilization, especially to disrupt biofilms formed by multidrug-resistant bacteria. However, the underlying molecular mechanism is not clear. In this study, CAP effectively reduced the living cells in the biofilms formed by methicillin-resistant Staphylococcus aureus, and 6 min treatment with CAP reduced the S. aureus cells in biofilms by 3.5 log10. The treatment with CAP caused the polymerization of SaFtsZ and SaClpP proteins in the S. aureus cells of the biofilms. In vitro analysis demonstrated that recombinant SaFtsZ lost its self-assembly capability, and recombinant SaClpP lost its peptidase activity after 2 min of treatment with CAP. Mass spectrometry showed oxidative modifications of a cluster of peaks differing by 16 Da, 31 Da, 32 Da, 47 Da, 48 Da, 62 Da, and 78 Da, induced by reactive species of CAP. It is speculated that the oxidative damage to proteins in S. aureus cells was induced by CAP, which contributed to the reduction of biofilms. This study elucidates the biological effect of CAP on the proteins in bacterial cells of biofilms and provides a basis for the application of CAP in the disinfection of biofilms.

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“…Many microorganisms live in the environment as biofilms rather than free-living organisms. Biofilm was defined as the cells adhering to a solid surface and surrounded by an extracellular matrix produced by them (Czapka et al 2018). Such populations exhibit higher resistance to adverse external factors (antibiotics, temperature, and pH); therefore, they pose a serious challenge in both medicine and food industry (Maciejewska et al 2016).…”

mentioning

confidence: 99%

The State of Research on Antimicrobial Activity of Cold Plasma

Niedźwiedź

Waśko

Pawłat

et al. 2019

Polish Journal of Microbiology

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Microbiological contamination is a big challenge to the food industry, medicine, agriculture, and environmental protection. For this reason, scientists are constantly looking for alternative methods of decontamination, which ensure the effective elimination of unwanted biological agents. Cold plasma is a new technology, which due to its unique physical and chemical properties becomes a point of interest to a growing group of researchers. The previously conducted experiments confirm its effective action, e.g. in the disinfection of skin wounds, air, and sewage treatment, as well as in food preservation and decontamination. The reactive compounds present in the plasma: high-energy electrons, ionized atoms and molecules, and UV photons are the key factors that cause an effective reduction in the number of microorganisms. The mechanism and effectiveness of the cold plasma are complex and depend on the process parameters, environmental factors and the type and properties of the microorganisms that are to be killed. This review describes the current state of knowledge regarding the effectiveness of the cold plasma and characterizes its interaction with various groups of microorganisms based on the available literature data.

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Influence of Atmospheric Pressure Non-thermal Plasma on Inactivation of Biofilm Cells

Cited by 22 publications

References 45 publications

Impact of Plasma Pre-Treatment on the Tribological Properties of DLC Coatings on PDMS Substrates

Impact of Plasma Pre-Treatment on the Tribological Properties of DLC Coatings on PDMS Substrates

Cold Atmospheric-Pressure Plasma Caused Protein Damage in Methicillin-Resistant Staphylococcus aureus Cells in Biofilms

The State of Research on Antimicrobial Activity of Cold Plasma

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