Influence of arginine on the growth, arginine metabolism and amino acid consumption profiles of<i>Streptococcus thermophilus</i>T1C2 in controlled pH batch fermentations

Huang, Scott L.; Ai, Z.W.; Sun, Xiaomei; Liu, G.F.; Zhai, Shuang; Zhang, M.; Chen, H.; Feng, Zhen

doi:10.1111/jam.13221

Cited by 24 publications

(28 citation statements)

References 29 publications

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“…The results of the current study showed that ST-MZ-2 does not have the ability to synthesize arginine through a de novo pathway. The expression levels of astB and ADC , and changes in arginine concentration is consistent with our previous research results 3,12 . N-acetyl-ornithine has been found to protect against osmotic stress in some microorganisms 20 , but whether N-acetyl-ornithine helps ST-MZ-2 resist osmotic stress needs further study.…”

Section: Discussionsupporting

confidence: 92%

“…However, the reasons for the functions of the abovementioned AAs and their metabolic pathways in S. thermophilus have not been fully studied. Our previous study showed that the consumption of some AAs notably exceeds the necessary amounts for the growth of S. thermophilus , implying a significant overconsumption of AAs 3,12 . Based on our results and those of previous studies, we proposed the following series of questions: (i) How are these AAs metabolized in S. thermophilus ?…”

Section: Introductionmentioning

confidence: 97%

“…Furthermore, the accumulation of sodium lactate results in increases in the extracellular osmotic pressure. Thus, S. thermophilus encounters two stress factors simultaneously, namely, low intracellular pH and high extracellular osmotic pressure, particularly during the exponential growth phase 3,4 .…”

Section: Introductionmentioning

confidence: 99%

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Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations

Qiao

Liu

Leng

et al. 2018

Sci Rep

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Elucidating the amino acid (AA) metabolism patterns of Streptococcus thermophilus has important effects on the precise design of nitrogen sources for high-cell-density culture. Transcriptomics and metabolomics were combined to reveal the cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine and alanine metabolic pathways in S. thermophilus MN-ZLW-002, including glutathione. The changes in the synthesis, consumption and concentration of AAs and their metabolites, as well as regulatory genes with time were revealed. The metabolism of L-cysteine, L-glutamate, L-aspartate and L-alanine generated some potential functional metabolites. The metabolism of methionine and glutamate generated potential harmful metabolites. S. thermophilus MN-ZLW-002 can synthesize glutathione. Some potential functional metabolites have similar biological functions, indicating that S. thermophilus can resist environmental stresses through multiple mechanisms. The expression of some key genes in synthesis pathway of AA indicated that cysteine, methionine, asparagine, aspartate, arginine and lysine were insufficient or imbalance between nutrient components. The accumulation of large amounts of AA metabolites might be the primary cause of the overconsumption of AAs and influence the growth of S. thermophilus. The present study revealed the metabolic profiles of abovementioned AAs as well as those of regulatory genes and metabolites. These results were beneficial to the precise design of nitrogen sources and regulation of functional metabolites for the high-cell-density culture of S. thermophilus.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 97%

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Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations

Qiao

Liu

Leng

et al. 2018

Sci Rep

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“…S. thermophilus encounters two stress factors simultaneously during culturing, including the gradual increase in intracellular acidity and extracellular osmotic pressure (Shan et al, 2016;Zhengwen et al, 2017), which inhibit the growth and metabolic activities of LAB by affecting the uptake of nutrients, causing damage to membrane integrity and the synthesis of proteins and nucleic acids (Béal, Marin, Fontaine, Fonseca, & Obert, 2008;Konings & Otto, 1983;Savoie, Champagne, Chiasson, & Audet, 2007). Furthermore, we inferred that changes in stress might result in the efficient utilization of nutrients at T2 and T3, which was lower at lag phase and stationary phase.…”

Section: Discussionmentioning

confidence: 99%

“…Their concentrations may be unfit for the growth of ST-MZ-2 and should be increased in the media. The excessively high initial concentration of nutrients may inhibit growth of LAB (Shan et al, 2016). Nutrients with low consumption ratios and high residual concentrations, such as Pro, Ala, Ser, and most ions, were observed during the growth of ST-MZ-2.…”

Section: +mentioning

confidence: 99%

Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations

et al. 2018

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Effects of different carbon sources on metabolic profiles of carbohydrates in Streptococcus thermophilus during fermentation

et al. 2022

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BACKGROUND Streptococcus thermophilus is a major starter used in the dairy industry and it could improve the flavor of fermented products. It is necessary to improve biomass of S. thermophilus for its application and industrialization. The utilization of carbon sources directly affects the biomass of S. thermophilus. Therefore, the carbohydrate metabolism of S. thermophilus should be investigated. RESULTS In the present study, metabolic parameters and gene expression of S. thermophilus S‐3 with different carbon sources were investigated. The physicochemical results showed that S. thermophilus S‐3 had high lactose utilization. Transcriptome analysis found that approximately 104 genes were annotated onto 15 carbohydrate metabolic pathways, of which 15 unigenes were involved in the phosphotransferase system and 75 were involved in the ATP‐binding cassette transporter system. In addition, 171 differentially expressed genes related to carbohydrate metabolism were identified. Expression of the galactose metabolism genes lacSZ and galKTEM increased significantly from the lag phase to the mid‐exponential growth phase as a result of the global regulator protein, catabolite control protein A (CcpA). The high expression of galK in the mid‐ to late‐ phases indicated that the metabolite galactose is re‐transported for intracellular utilization. CcpA regulation may also induce high expressions of glycolytic pathway regulated‐genes related to lactose utilization, including ldh, fba, eno, pfkA, bglA, pgi, pgm and pyk, producing optimal glycolytic flux and S. thermophilus S‐3 growth. CONCLUSION The present study provides new insights into the carbon metabolism regulation and provide theoretical support for high‐density fermentation of S. thermophilus S‐3. © 2022 Society of Chemical Industry.

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Influence of arginine on the growth, arginine metabolism and amino acid consumption profiles ofStreptococcus thermophilusT1C2 in controlled pH batch fermentations

Abstract: The arginine-stimulated growth of Strep. thermophilus improved the utilization efficiency of amino acids and reduced nitrogen waste, which could be useful for the optimization of cultivation media.

Cited by 24 publications

References 29 publications

Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations

Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations

Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations

Effects of different carbon sources on metabolic profiles of carbohydrates in Streptococcus thermophilus during fermentation

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