Influence of apple phytochemicals in ZnO nanoparticles formation, photoluminescence and biocompatibility for biomedical applications

Alves, Marta M.; Andrade, Suzana M.; Grenho, Liliana; Fernandes, Maria Helena; Santos, Catarina; Montemor, M.F.

doi:10.1016/j.msec.2019.03.084

Cited by 40 publications

(29 citation statements)

References 63 publications

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“…1 h) indicates an average fluorescence lifetime of 5 ns, with low background contamination. This lifetime is in the same order of magnitude of previously determined values for ZnO 31 .…”

Section: Resultssupporting

confidence: 87%

“…When analysing the electron diffraction (ED) pattern of ZnO NRs agglomerates, the presence of the ZnO crystalline planes (1 0 0), (0 0 2), (1 1 0), (1 0 2), (1 0 3) and (1 1 2) ( Fig. 1f) confirms the presence of pure wurtzite crystals, as typically reported for these ZnO crystals 31 .…”

Section: Resultssupporting

confidence: 74%

“…Fluorescence lifetimes were detected with a single-photon avalanche diode (SPAD) (PerkinElmer), and the signal was processed by a Time Harp 200 TC-SPC PC board (PicoQuant) working in time-tagged time-resolved (TTTR) operation mode. For point-by-point measurements, fluorescence decays of > 30 pixel points were collected 31 . The peaks position on the PL spectra in the green and red emission ranges were deconvoluted into one or more components through the Gaussian curve-fitting method used in Origin 8.0 (OriginLab Corporation).…”

Section: Photoluminescence and Fluorescence Measurements A Perkin Elmentioning

confidence: 99%

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Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

Babu

Pinheiro

Marques

et al. 2020

Sci Rep

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Cancer is the second cause of death worldwide. This devastating disease requires specific, fast, and affordable solutions to mitigate and reverse this trend. A step towards cancer-fighting lies in the isolation of natural killer (NK) cells, a set of innate immune cells, that can either be used as biomarkers of tumorigenesis or, after autologous transplantation, to fight aggressive metastatic cells. In order to specifically isolate NK cells (which express the surface NKp30 receptor) from peripheral blood mononuclear cells, a ZnO immunoaffinity-based platform was developed by electrodeposition of the metal oxide on a flexible indium tin oxide (ITO)-coated polyethylene terephthalate (PET) substrate. The resulting crystalline and well-aligned ZnO nanorods (NRs) proved their efficiency in immobilizing monoclonal anti-human NKp30 antibodies (mAb), obviating the need for additional procedures for mAb immobilization. The presence of NK cells on the peripheral blood mononuclear cell (PBMCs) fraction was evaluated by the response to their natural ligand (B7-H6) using an acridine orange (AO)-based assay. The successful selection of NK cells from PBMCs by our nanoplatform was assessed by the photoluminescent properties of AO. This easy and straightforward ZnO-mAb nanoplatform paves the way for the design of biosensors for clinic diagnosis, and, due to its inherent biocompatibility, for the initial selection of NK cells for autotransplantation immunotherapies.

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“…1 h) indicates an average fluorescence lifetime of 5 ns, with low background contamination. This lifetime is in the same order of magnitude of previously determined values for ZnO 31 .…”

Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 74%

Section: Photoluminescence and Fluorescence Measurements A Perkin Elmentioning

confidence: 99%

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Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

Babu

Pinheiro

Marques

et al. 2020

Sci Rep

Self Cite

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“…The synthesized NPS were filtered with a PM UC500 filter (Microdyn Nadir, Wiesbaden, Germany). The collected particles were dried overnight in a conventional oven (WTB binder, Munich, Germany) at 70 • C [1,38].…”

Section: Zno Nanoparticles (Nps) Synthesismentioning

confidence: 99%

Biodegradable Chitosan Films with ZnO Nanoparticles Synthesized Using Food Industry By-Products—Production and Characterization

et al. 2021

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This work aimed to produce bionanocomposites of chitosan incorporated with zinc oxide nanoparticles (ZnO NPs) synthesized using food industry by-products and to characterize them. Such nanoparticles are highlighted due to their low cost, antimicrobial activity, accessibility, and sustainability synthesis. Four different levels of ZnO NPs (0, 0.5, 1.0, and 2.0% w/w of chitosan) were tested, and the bionanocomposites were characterized in terms of their hydrophobicity, mechanical, optical, and barrier properties. Overall, the incorporation of ZnO NPs changed the composites from brittle to ductile, with enhanced elongation at break and reduced Young Modulus and tensile strength. Thus, ZnO NPs acted as plasticizer, turning the films more flexible, due to the presence of organic compounds on the NPs. This also favored permeability of oxygen and of water vapor, but the good barrier properties were maintained. Optical properties did not change statistically with the ZnO NPs incorporation. Thus, the characterization presented in this paper may contribute to support a decision on the choice of the material’s final application.

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“…Annealing in vacuum effectively decreases impurities in samples. The photoluminescent properties, revealed that besides the intense sharp near-UV band edge emission, a strong broad emission band peak at 2.20 eV was detected for nanoparticles associated to crystal defects characterized by steady-state and time resolve photoluminescence measurements(Alves et al, 2019). DLS) is routinely used for nanoparticle size determination, size distribution and zeta potential in solution.…”

mentioning

confidence: 99%

Plant Mediated Green Synthesis of Zinc Oxide Nanoparticles for Burn Wound Therapy

Beg¹,

Ghodinde²,

Gupta³

2020

Preprint

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In this changing world, we all are surrounded by the surmountable risk of getting injured. Amongst various risk factors, major burns are the most distressing and catastrophic. Burn wounds are not easy to heal via natural healing process and ultimately ended up with scar formation. If the degree of burn is high then the loss of tissue and its function is very common. To fasten-up the natural burn wound healing; zinc, an essential trace element is found to be very much effective. But due to its’ particle size limitation, less contact with wounded cells and tissues, and high inherent toxicity restrict its use. Needlessly, zinc is an element with dual action i.e. both antimicrobial and wound healing it is a prime choice to apply its aptitude in burn wound healing. To overcome the documented limitations zinc has converted to nanoparticle form. Zinc oxide nanoparticles, in particular, have attained ample of interest due to their unique properties and potential antimicrobial activity along with wound healing activity which makes it promising for the healing of topical burn wounds. Plant mediated green synthesis of nano-metal oxide particles is gaining a lot of significance due to its simplicity, eco-friendliness and extensive antimicrobial activity and recommended as an appealing substitute to not only physical methods but also chemical methods avoiding the use of the high rate of toxic chemicals and extreme surroundings. This study includes ZnO NPs role in burn wound healing with Phyto-mediated synthesis methods to provide evidence of their potential applications. Additionally, it provides an overview of traditional methods used for the synthesis of ZnO nanoparticles and characterization techniques to obtain information concerning the size, shape and optical properties along with toxicity and safety concern of ZnO NPs and its biomedical applications.

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Influence of apple phytochemicals in ZnO nanoparticles formation, photoluminescence and biocompatibility for biomedical applications

Cited by 40 publications

References 63 publications

Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

Biodegradable Chitosan Films with ZnO Nanoparticles Synthesized Using Food Industry By-Products—Production and Characterization

Plant Mediated Green Synthesis of Zinc Oxide Nanoparticles for Burn Wound Therapy

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