Influence of apo‐ and iron‐saturated lactoferrin and transferrin, immunoglobulin G and serum albumin on proliferation of bovine peripheral blood mononuclear cells

Abstract

1992) Influence of apo-and iron-saturated lactoferrin and transferrin, immunoglobulin G and serum albumin on proliferation of bovine peripheral blood mononuclear cells, Food and Agricultural Immunology, 4:4, 253-257, Whey proteins from non-lactating bovine mammary secretions reduced peripheral blood mononuclear cell (PBMC) response to mitogens. Specific proteins responsible for hyporesponsiveness are unknown. This study evaluated the influence of apo-and ironsaturated lactoferrin (APOLF and FELF) and transferrin (APOTF and FETF), immunoglobulin G (IgG) and serum albumin (SA) at concentrations ranging from 0 02 to 2 5 mg ml -1 on PBMC response to concanavalin A. PBMC were collected from five pregnant Holstein cows during late lactation. Lactoferrin (LF), transferrin (TF) and IgG resulted in a dose-dependent reduction of PBMC proliferation, whereas SA had no effect. Decreased PBMC response was significant at all concentrations of APOLF and FELF, at APOTF concentrations 0.3 mg ml -1 , only at 2·5 mg ml -1 FETF, and at IgG concentrations 1·25 mg ml -1 . The degree of iron saturation of LF did not influence PBMC response; however, APOTF had a greater influence than FETF. Reduced PBMC response at low levels of LF indicate that hyporesponsiveness is not due solely to an increased protein content of the culture but may be the result of specific LF regulation of mononuclear cell proliferation.

Inhibition of proliferative responses of mouse spleen lymphocytes by lacto‐ and ovotransferrins

“…Downloaded by [University of Saskatchewan Library] at 10:34 19 November 2014 Slater and Fletcher (1987) reported that human holo-lactotransferrin inhibited the uptake of tritiated uridine by allogenic mixed lymphocyte cultures whereas apo-lactotransferrin did not. A significant difference between the influence of apo-serotransferrin and holo-serotransferrin on Con A-induced bovine blood lymphocyte proliferation was observed (Rejman et al, 1992). In the present work, however, apo-bLf, cOf and qOf were as effective as holo-transferrins to cause cytotoxicity for mouse lymphocytes and to inhibit the proliferation of mouse lymphocytes (Figure 2).…”

“…Ashorn et al (1986) and Richie et al (1987) showed that human lactotransferrin suppressed the Con A-and PHA-induced proliferation respectively of human lymphocytes. Rejman et al (1992) observed that bovine serotransferrin and lactotransferrin suppressed the Con A-induced proliferation of bovine peripheral blood lymphocytes. More recently, Otani et al (1995b) reported that quail egg-white ovotransferrin inhibited Con Aand PHA-induced proliferation of mouse lymphocytes.…”

Modulation of immune function by a modified bovine whey protein concentrate

“…It is known that beta‐and kappa‐casein (in particular the carbohydrate‐rich glycomacropeptide component of the latter) have a variety of suppressive and stimulatory effects on mononuclear cell function 2 , 3 , 5 , 6 , 7 , 8 , 9 . In addition, some of the proteins isolated and purified from whey have been shown to be potent modulators of cellular immune function; these include lactoperoxidase, lactoferrin, and milk growth factor 4 , 8 , 10 , 11 . In general, the immunomodulatory function of these individual components becomes more evident (and the dose response more potent) as they are progressively purified from whole casein or whey 8 .…”

Enhancement of mammary gland mononuclear cell proliferation by interleukin‐2 in the presence of lactoferrin

“…The proliferation of PBMCs in response to mitogens decreased when they were cultured in the presence of mammary secretions (Torre & Oliver, 1989). Recent evidence identified LF, a major component of bovine mammary secretions during the non-lactating period (Welty et ah, 1976), as having the greatest negative influence on response of PBMC to Con A (Rejman et al, 1992). Richie et a/.…”

“…Intact mammary secretions obtained during the non-lactating period elicited a greater reduction of PBMC proliferation in response to mitogens than secretions from lactating cows (Torre & Oliver, 1989). In similar experiments using purified mammary secretion whey proteins, lactoferrin (LF) was associated with the greatest reduction of PBMC proliferation in response to concanavalin A (Con A; Rejman et al, 1992). Thus, the environment in which mononuclear cells must function may significantly influence their response.…”