Influence of Antioxidant Effect of Stobadine Derivative in Condition of Kidney Ischemia-Reperfusion in a Pre-Clinical Experiment (Effect in Prophylaxis)

Abstract

The goal of the study was to monitor the antioxidative effect of stobadine derivative in the conditions of ischemia-reperfusion of laboratory rat kidney tissue. The animals were divided by random selection into 5 groups (n = 10). The treated groups were given stobadine derivate in peroral doses of 5, 10 and 20 mg/kg in 0.5 % solution of Avicel once a day; the placebo group was given only the solution of Avicel. The last group was an intact group (without ischemia-reperfusion and without treatment). After conclusion of medication on the 15th day all animals were subjected to kidney tissue ischemia (60 min.) followed by reperfusion (10 min.). All animals were subsequently exsanquined and single identification of superoxiddismutase, glutathion peroxidase, total antioxidative capacity, and malondialdehyde level in the blood were determined. Kidneys were recovered for histopathological examination. A statistically significant decrease of the superoxiddismutase and statistically significant increase of the glutathione peroxidase catalytic activity in the treated groups compared to the groups of placebo and intact was discovered. There was also a statistically highly significant increase of total antioxidative capacity in the treated groups compared to the groups of placebo and intact. A statistically significant decrease of malondialdehyde level was identified in the treated groups compared to the groups of placebo and intact. The results of biochemical examination show a protective antioxidative effect of stobadine derivative. The results of histopathological examination support this assumption.

Stobadine Protects Rat Kidney Against Ischaemia/Reperfusion Injury

“…Ischaemia–reperfusion injury is one of the main causes of acute renal failure and the clinical diagnosis is usually made after the injury has already occurred. Previous studies have demonstrated a promising effect of oral heptanoylstobadine administration against renal I/R injury 14,15 . In these previous studies, heptanoylstobadine was administered 15 days before I/R (to examine its prophylactic effect) or after 60 min ischaemia and 10 min reperfusion (to examine its therapeutic effect).…”

“…Previous studies have demonstrated a promising effect of oral heptanoylstobadine administration against renal I/R injury. 14,15 In these previous studies, heptanoylstobadine was administered 15 days before I/R (to examine its prophylactic effect) or after 60 min ischaemia and 10 min reperfusion (to examine its therapeutic effect). Although the anti-oxidant effects of stobadine in the kidney were demonstrated in these previous studies, no beneficial effects were noted in terms of serum urea and creatinine, which are common physiologial markers of kidney function.…”

“…It has been shown that stobadine also has protective effects in the myocardium, aorta 13 and neural tissue against I/R and hypoxia/re‐oxygenation injury or in the presence of chemical systems that generate free oxygen radicals 9 . To the best of our knowledge, only oral administration of the acyl derivative of stobadine, namely heptanoylstobadine, has been assessed as a treatment against renal I/R injury and these studies have used different methodological approaches 14,15 …”

“…9 To the best of our knowledge, only oral administration of the acyl derivative of stobadine, namely heptanoylstobadine, has been assessed as a treatment against renal I/R injury and these studies have used different methodological approaches. 14,15 The aim of the present study was to evaluate the effect of the intravenous administration of stobadine on renal function and morphology, serum and kidney levels of MDA, GSH and GSHdependent enzymes and the pentose phosphate pathway enzymes after 40 min ischaemia and 6 h reperfusion in rats in vivo.…”

The Effect of Statins and Other Cardiovascular Medication on Ischemia-Reperfusion Damage in a Human DIEP Flap Model: Theoretical and Epidemiological Considerations

“…However, it significantly attenuated lipid peroxidation [ 18 ]. Stobadine, a pyridoindole derivative that displays cardioprotective and antiarhythmical effects, showed a protective effect during I/R by increasing the glutathione peroxidase activity and the total antioxidant capacity [ 36 ]. ACE inhibitors and calcium antagonists, especially the dihydropyridines, possess radical scavenging properties and prevent ROS formation.…”