Influence of Antigen Insertion Site and Vector Dose on Immunogenicity and Protective Capacity in Sendai Virus-Based Human Parainfluenza Virus Type 3 Vaccines

Mason, John N.; Elbahesh, Husni; Russell, Charles J.

doi:10.1128/jvi.00227-13

Cited by 8 publications

(6 citation statements)

References 55 publications

(76 reference statements)

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“…The basis of this disproportionate effect is unknown and potentially could involve various factors such as altered protein stoichiometry, disturbed gene expression gradient, and the possible interference with an unidentified cis-acting element. It has been reported that insertion at the comparable (P-M) position of Sendai virus similarly resulted in greater growth restriction than other positions (43)(44)(45). This suggests a possible parallel between these two paramyxoviruses, but this remains to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Chimeric Bovine/Human Parainfluenza Virus Type 3 Expressing Respiratory Syncytial Virus (RSV) F Glycoprotein: Effect of Insert Position on Expression, Replication, Immunogenicity, Stability, and Protection against RSV Infection

Liang

Munir

Amaro-Carambot

et al. 2014

J Virol

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The research findings presented here will be instrumental for improving the design of a bivalent pediatric vaccine for respiratory syncytial virus and parainfluenza virus type 3, two major causes of severe respiratory tract infection in infants and young children. Moreover, this knowledge has general application to the development and clinical evaluation of other mononegavirus vectors and vaccines.

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Section: Discussionmentioning

confidence: 99%

Chimeric Bovine/Human Parainfluenza Virus Type 3 Expressing Respiratory Syncytial Virus (RSV) F Glycoprotein: Effect of Insert Position on Expression, Replication, Immunogenicity, Stability, and Protection against RSV Infection

Liang

Munir

Amaro-Carambot

et al. 2014

J Virol

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“…NHBE cells, purchased from Lonza (MD, USA), were donor cells from a single 4-year-old healthy male and were prepared as previously described (33). (Table 1) were obtained from the St. Jude Children's Research Hospital repository and were grown in 10-day-old embryonated chicken eggs for 48 h at 37°C.…”

Section: Cellsmentioning

confidence: 99%

“…SDS-PAGE and Western blot analyses were performed as described previously (33). Separated proteins were transferred to nitrocellulose, blocked, and probed with specific primary antibodies and peroxidase-labeled secondary antibody.…”

Section: Viral Infectionsmentioning

confidence: 99%

Novel Roles of Focal Adhesion Kinase in Cytoplasmic Entry and Replication of Influenza A Viruses

Elbahesh

Cline

Baranovich

et al. 2014

J Virol

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Viruses IMPORTANCEWe found that FAK links early activation of PI3K and actin reorganization, thereby regulating influenza virus entry. Surprisingly, we also found that FAK can regulate viral RNA replication independently of its role in entry. Our study addresses a knowledge gap in the understanding of signaling events triggered by influenza virus that mediate its internalization and initiation of the infection cycle. Understanding of these fundamental molecular events will be necessary to identify novel host targets, such as FAK, and development of future anti-influenza virus therapeutics.

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“…L ive virus vaccines have proven to be effective for driving CD8 + T-cell responses for therapy to treat a variety of diseases, making them appealing as vectors for antigen-specific immunotherapy (14,16,24,28,30). One of the major limitations to their efficacy, however, is the induction of immunity to vector antigens rather than recombinant target antigens (26,29).…”

mentioning

confidence: 99%

A CD8 T-Cell Epitope Variant Enhances Immune Targeting to a Recombinant Picornavirus Vaccine Antigen

et al. 2014

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Recombinant virus vaccines are often less effective due to immunodominant responses against endogenous vector antigens. However, the use of small RNA virus vectors provides an opportunity to limit host exposure to endogenous virus antigens and focus immune responses on the desired vaccine antigen. Using the Daniel's strain of Theiler's murine encephalomyelitis virus, we have identified strategies to modulate responses to endogenous viral proteins by manipulating the host CD8+ T-cell repertoire prior to infection or through the use of mutations introduced into the virus genome. Both of these approaches enhance responses to vaccine antigens introduced into the picornavirus. However, the use of mutant immunodominant epitopes provides an opportunity for enhancing vaccine responses without further manipulation of the host. Using this strategy, we demonstrate that modification of the consensus MHC class I anchor residue within the virus genome can promote enhanced immunity to foreign antigens and self-antigens embedded in the virus genome.

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Influence of Antigen Insertion Site and Vector Dose on Immunogenicity and Protective Capacity in Sendai Virus-Based Human Parainfluenza Virus Type 3 Vaccines

Cited by 8 publications

References 55 publications

Chimeric Bovine/Human Parainfluenza Virus Type 3 Expressing Respiratory Syncytial Virus (RSV) F Glycoprotein: Effect of Insert Position on Expression, Replication, Immunogenicity, Stability, and Protection against RSV Infection

Chimeric Bovine/Human Parainfluenza Virus Type 3 Expressing Respiratory Syncytial Virus (RSV) F Glycoprotein: Effect of Insert Position on Expression, Replication, Immunogenicity, Stability, and Protection against RSV Infection

Novel Roles of Focal Adhesion Kinase in Cytoplasmic Entry and Replication of Influenza A Viruses

A CD8 T-Cell Epitope Variant Enhances Immune Targeting to a Recombinant Picornavirus Vaccine Antigen

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