Influence of angiogenin on the growth of A375 human melanoma cells and the expression of basic fibroblast growth factor

Song, Jian; Wang, Jun; Yang, Jing; Jiang, Chunhua; Shen, Wei; Wang, Li

doi:10.1097/01.cmr.0000215029.62199.4c

Cited by 10 publications

(10 citation statements)

References 24 publications

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“…Kishimoto et al reported that the expression of bFGF increased in angiogenin antisense transfected HeLa cells (24). Song et al found that the angiogenin sense transfected A375 actually expressed a lower amount of bFGF, and the angiogenin antisense transfectants expressed a higher amount of bFGF than the control cells (25). It seems that bFGF and angiogenin could interact, although the mechanism is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Kishimoto et al have observed that angiogenin antisense HeLa transfectants and tumors in athymic mice actually expressed a higher amount of bFGF than the control cells and tumors in athymic mice (24). Song et al demonstrated that the angiogenin sense transfectants actually expressed a lower amount of bFGF, and the angiogenin antisense transfectants expressed a higher amount of bFGF than A375 cells (25). To further understand the biological role of angiogenin and bFGF, we investigated the influence of bFGF on angiogenin gene expression and angiogenin secretion in HeLa cell lines.…”

Section: Introductionmentioning

confidence: 99%

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Influence of basic fibroblast growth factor on the growth of HeLa cells and the expression of angiogenin

Yang

Wang²,

Zhao³

et al. 2009

Oncol Rep

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Abstract. Basic fibroblast growth factor (bFGF) is closely involved in angiogenesis and tumor growth of various cancers, but its role in cervical cancer remains to be defined. We investigated the effects of bFGF on HeLa cell growth and studied its influence on the expression of angiogenin. We transfected the bFGF gene in the sense and antisense orientation into HeLa cells, and obtained stable bFGF underexpressing and overexpressing transfectants. In our experiments, we demonstrated that inhibition of bFGF gene and protein expression in the bFGF antisense transfectants induced increased protein expression of angiogenin. In contrast, in the bFGF sense transfectants the expression of angiogenin decreased. Delivery of recombinant angiogenin into transfected and control cells led to increased proliferation in the bFGF antisense transfectants and the control cells. However, the cell proliferation had no change in the bFGF sense transfectants. In conclusion, we demonstrated that besides its angiogenic activity, bFGF and angiogenin also directly contribute to HeLa cell proliferation. Furthermore, endogenous bFGF affects the expression of angiogenin in HeLa cells. These findings suggest that inhibition of bFGF alone is not a promising strategy to inhibit angiogenesis. IntroductionCancer of the uterine cervix is the second most common cancer among women in the world (1), and the most common gynecological malignancy in P.R. China. Angiogenesis stimulates tumor growth, invasion and metastasis, and therefore is a crucial step in tumorigenesis. Further, evidence of increased neovascularization has been shown to be a negative prognostic indicator in many solid tumors, including cervical cancer (2). Several growth factors may be involved in the angiogenic process, including angiogenin and basic fibroblast growth factor (bFGF).Angiogenin is a 14-kDa angiogenic protein originally isolated from the conditioned medium of HT-29 human colon adenocarcinoma cells (3). Angiogenin has been shown to play a role in tumor angiogenesis (4). Its expression is upregulated in many types of cancers (5-10). Angiogenin undergoes nuclear translocation in endothelial cells, which has been shown to be necessary for angiogenesis (11-13). Tsuji et al have shown that nuclear translocation of angiogenin occurred in HeLa cells regardless of the cell confluence status, and that angiogenin stimulated rRNA synthesis in HeLa nuclei (14). Many studies have focused on how angiogenin induced tumor angiogenesis and how its angiogenic activity can be disrupted. Little, however, is known about its expression and function during HeLa cell growth.Basic fibroblast growth factor (bFGF), a member of a group of heparin-binding multifunctional polypeptides, is one of the most potent angiogenic factors (15,16). It is also involved in proliferation and differentiation of a variety of normal and malignant cells and tissues (17,18). Many malignant tumors showed an increase in bFGF expression (19)(20)(21)(22). Sliutz et al have demonstrated that serum basic fibroblast growth f...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of basic fibroblast growth factor on the growth of HeLa cells and the expression of angiogenin

Yang

Wang²,

Zhao³

et al. 2009

Oncol Rep

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“…The expression of Ang is up-regulated in numerous tumors (Tello-Montoliu et al, 2006), and also found in normal cells (Fernández-Santiago et al, 2009). Isolation of Ang from normal human plasma demonstrates that Ang is not a tumorspecific product (Song et al, 2006), but may also have some other biological activities in addition to angiogenesis.…”

Section: Introductionmentioning

confidence: 98%

Identification and characterization of FHL3 as a novel angiogenin-binding partner

Xia¹,

Fu²,

Cai³

et al. 2012

Gene

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“…In melanoma cell line, basic fibroblast growth factor cannot induce proliferation in the absence of Angiogenin (Song et al 2006). Knock down studies in HeLa cells showed notable inhibition of proliferation, and in prostate adenocarcinoma cells, its knock down caused inhibition of rRNA transcription, cell proliferation, and xenograft growth in mice (Tsuji et al 2005;Yoshioka et al 2006).…”

Section: I) Angiogeninmentioning

confidence: 99%

Characterizing the endoribonuclease activity of APE1.

Kim¹

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Influence of angiogenin on the growth of A375 human melanoma cells and the expression of basic fibroblast growth factor

Cited by 10 publications

References 24 publications

Influence of basic fibroblast growth factor on the growth of HeLa cells and the expression of angiogenin

Influence of basic fibroblast growth factor on the growth of HeLa cells and the expression of angiogenin

Identification and characterization of FHL3 as a novel angiogenin-binding partner

Characterizing the endoribonuclease activity of APE1.

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