Influence of anaesthesia and surgery on neutrophil chemotaxis

Moudgil, G. C.; Pandya, Aastha Rajendra; Ludlow, David

doi:10.1007/bf03005506

Cited by 39 publications

(9 citation statements)

References 19 publications

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“…It has been known for almost a century that anesthetic agents can affect aspects of the immune response [5][6][7], Specific studies have shown that anesthesia can suppress specific components of neutrophil function, including neutrophil chemotaxis [24,25], phagocytosis [26][27][28], bacterial killing capacity [29,30], and superoxide generation [27, 28. 31].…”

Section: Discussionmentioning

confidence: 99%

Suppression of Cytokine-lnduced Neutrophil Accumulation in Rat Mesenteric Venules in vivo by General Anesthesia

Miller¹,

Morita²,

Rangan³

et al. 1996

Int J Microcirc

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Most studies of neutrophil-endothelial interactions in vivo necessarily require the use of general anesthetic agents which are well known to be immunosuppressive. By using whole-mount preparations of the rat mesoappendix, we were able to study tumor necrosis factor alpha (TNF-α) induced neutrophil adhesion to the mesenteric venular endothelium in vivo without necessarily using general anesthesia. TNF-α significantly increased venular-neutrophil accumulation in a dose-dependent manner; accumulation was markedly increased at 1,2, and 4 h, but returned to baseline after 24 h. After these preliminary dose-response and time-course studies, we evaluated the influence of standard clinically effective doses of several commonly used anesthetic agents (thiopental, pentobarbital, ketamine, α-chloralose, methoxyflurane, and halothane) on the extent of neutrophil-venular accumulation induced 2 h after intraperitoneal injection of 0.4 mg/kg TNF-α, compared to unanesthetized rats. All general anesthetics tested, with the exception of methoxyflurane, significantly suppressed this response. In most cases this suppression was striking (from 60 to 85%) such that a statistically significant proinflammatory response was obscured. Although methoxyflurane also tended to suppress this response to TNF-α, it was the only agent that allowed the response to be clearly seen. Because anesthesia markedly suppresses cytokine-induced neutrophil-venular adhesion, this model should provide an important complementary technique to the classical in vivo microcirculatory approaches which do necessarily require general anesthesia.

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Section: Discussionmentioning

confidence: 99%

Suppression of Cytokine-lnduced Neutrophil Accumulation in Rat Mesenteric Venules in vivo by General Anesthesia

Miller¹,

Morita²,

Rangan³

et al. 1996

Int J Microcirc

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“…Moudgil, Krunholz, Weiss and Skoultelia found that the phagocytosis and chemotaxis functions of neutrophils were suppressed when exposed to in vitro anaesthetics. As a result of decreased production of oxygen radicals, the intracellular killing process of bacteria is inhibited [15][16][17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Effects of Propofol and Midazolam on the Inflammation of Lungs after Intravenous Endotoxin Administration in Rats

et al. 2015

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Objective: Pulmonary complications are important sepsis (such as ARDS, diffuse pneumonia). Acute respiratory distress syndrome (ARDS) is characterized by the extensive migration of neutrophils into alveoli of the lungs. Propofol and midazolam are the most widely used agents for sedation in intensive care units. Aimed to investigate the effects of anaesthesia with propofol and midazolam on measured hemodynamic variables and neutrophil migration induced by Escherichia Coli endotoxin (ECE) in pulmonary viscera. Materials and Methods: Forty Sprague Dawley male rats were randomly assigned to four groups: Thiopental Sodium 30 mg/kg was administered intraperitoneally to anesthetize the rats. They were ventilated via tracheotomy. Femoral artery was cannulated for the measurement of continuous blood pressure and gases. Group C was the control. After the administration of 1 mL/kg 0.9% NaCL, infusion began at 1 mL/kg/h rate. In Group E 15 mg/kg lipopolysaccharide derived from ECE was administered iv. In Group PE, after a bolus dose of 10 mg/kg propofol and 15 mg/kg ECE, 10 mg/kg/h infusion was applied. In Group ME, after 0.1 mg/kg midazolam bolus dose and 15 mg/kg ECE administration, 0.1 mg/kg/h infusion was administered iv. Rats were sacrified by iv potassium chloride. The lungs were then removed, fixed in 10% buffered formalin for 3 days and embedded in paraffin. They were graded on a scale of 0-3 according to the aggregation of neutrophils. Results: There was intense neutrophil migration in Group E (grade 2, 3). However, although mild neutrophil migration was obtained in 70% of the rat lungs in Group ME (grade 1, 2), it was recorded in only 30% of Group PE (grade 1). Conclusion: The sepsis model induced by ECE and compared with midazolam, propofol anaesthesia is associated with less neutrophil infiltration. In the light of the literature, propofol attenuate the free-radical-mediated lipid peroxidation and systemic inflammation in patients. Keywords: Sepsis, lung, neutrophil, propofol, midazolam, rat ÖzetAmaç: Sepsiste akciğer komplikasyonları önemli bir yer tutar (ARDS, diffüz pnömoni gibi). Akut respiratuar distres sendromu (ARDS) akciğerde alveollere aşırı nötrofil migrasyonu ile karakterizedir. Propofol ve midazolam yoğun bakımlarda hasta sedasyonu için çok yaygın olarak kullanılan ilaçlardır. Escherichia Coli endotoksini ile oluşturulan sepsis modelinde propofol ve midazolam anestezisi altında akciğerlere nötro-fil göçünü ve hemodinamik parametreleri değerlendirmeyi amaçladık. Gereç ve Yöntem: 40 adet rat (Spraque-Dawley cinsi) rastgele dört gruba-ayrıldı. 1-Grup E (Endotoksemi grubu) 2-Grup K (Kontrol grubu) 3-Grup PE (Propofol-Endotoksemi grubu) 4-Grup ME (Midazolam-Endotoksemi grubu). Tüm deneklere intraperitoneal 30 mg/kg tiyopental sodyum verildi. Denekler trakestomi yoluyla ventile edildiler. Devamlı kan basıncının ölçülmesi ve kan örneklerinin alınabilmesi için femoral arter kanüle edildi. Grup E'deki deneklere E.Coli'den elde edilmiş lipopolisakkarit derivesi intravenöz olarak 15 mg/kg uygulandı. Gru...

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“…Many of these adverse effects result from the irreversible inhibition of vitamin B12 by N 2 O inhibition of methionine synthase, folate metabolism, and deoxyribonucleic acid synthesis is the result [29,30,31]. N 2 O also depresses chemotactic migration by neutrophils and monocytes, apparently by interfering with microtubules [32,33]. Increase in homocysteine concentration is another effect which may lead to increased myocardial infarction [34].…”

Section: The Adverse Effects Of Nitrous Oxidementioning

confidence: 99%

Nitrous oxide. Should it still be used in pediatric medicine ? (Part 1)

Booij¹

2017

Medical news of the North Caucasus

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Influence of anaesthesia and surgery on neutrophil chemotaxis

Cited by 39 publications

References 19 publications

Suppression of Cytokine-lnduced Neutrophil Accumulation in Rat Mesenteric Venules in vivo by General Anesthesia

Suppression of Cytokine-lnduced Neutrophil Accumulation in Rat Mesenteric Venules in vivo by General Anesthesia

Effects of Propofol and Midazolam on the Inflammation of Lungs after Intravenous Endotoxin Administration in Rats

Nitrous oxide. Should it still be used in pediatric medicine ? (Part 1)

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