Influence of ambient temperature and humidity on ATP1A1 gene expression in Tharparkar and Vrindavani cattle

Kashyap, Neeraj; Kumar, Pushpendra; Deshmukh, B. T.; Dige, Mahesh Shivanand; Sarkar, Mihir; Kumar, Anuj; Chauhan, Anuj; Singh, Gyanendra

doi:10.5958/0976-0555.2014.00028.4

Cited by 8 publications

(7 citation statements)

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“…When a cell experiences environmental stress, it stops or at least slows down most of its original functions, such as transport processes, DNA, RNA and protein synthesis. However, there are proteins which preferentially expressed under these, restrictive conditions like slick hair gene [ 8 ], ATP1A1 [ 9 , 10 ], ATP1B2 [ 11 ] and heat shock proteins (HSPs). HSPs are highly conserved proteins activated by numerous physiological and physical stressors [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle

Bhat¹,

Kumar²,

Kashyap³

et al. 2016

Vet World

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Aim:Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle.Materials and Methods:A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-single-strand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons.Results:Three SSCP patterns and consequently two alleles namely A and B were documented in one fragment of HSP70 gene. On sequencing, one single-nucleotide polymorphism with G > T substitution was found at a position that led to a change of amino acid aspartate to tyrosine in allele A. It was found that in maintaining near normal average rectal temperature, genotype AA was superior (p≤0.01). Genotype AA, thus, was found to be most thermotolerant genotype with the highest HTC (p≤0.01).Conclusion:The polymorphism at HSP70 is expected to be a potent determinant for heat tolerance in cattle, which may aid in selection for thermotolerance in cattle.

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Section: Introductionmentioning

confidence: 99%

Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle

Bhat¹,

Kumar²,

Kashyap³

et al. 2016

Vet World

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“…It has also been shown that certain genotypic variants of different Na + /K + -ATPase subunits have different heat tolerance in cows [ 25 - 28 ]. A differential expression of ATP1A1 in different seasons [ 29 ] is also suggestive of the gene to have a certain part in animals’ response to environmental attributes.…”

Section: Introductionmentioning

confidence: 99%

Association of ATP1A1 gene polymorphism with thermotolerance in Tharparkar and Vrindavani cattle

et al. 2015

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Aim:One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient across biological membranes. Na+/K+-ATPase, a member of P type-ATPase family, is a major contributor to the mechanism that actively controls cross-membrane ion gradient. Thus, we examined ATP1A1 gene that encodes alpha-1 chain of Na+/K+-ATPase, for genetic polymorphisms.Materials and Methods:A total of 100 Vrindavani (composite cross strain of Hariana x Holstein-Friesian/Brown Swiss/Jersey) and 64 Tharparkar (indigenous) cattle were screened for genetic polymorphism in ATP1A1 gene, using polymerase chain reaction single-strand conformation polymorphism and DNA sequencing. For association studies, rectal temperature (RT) and respiration rate (RR) of all animals were recorded twice daily for 3 seasons.Results:A SNP (C2789A) was identified in exon 17 of ATP1A1 gene. Three genotypes namely CC, CA, and AA were observed in both, Vrindavani and Tharparkar cattle. The gene frequencies in Tharparkar and Vrindavani for allele A were 0.51 and 0.48, and for allele C were 0.49 and 0.52, respectively, which remained at intermediate range. Association study of genotypes with RT and RR in both cattle population revealed that the animals with genotype CC exhibited significantly lower RT and higher heat tolerance coefficient than CA and AA genotypes.Conclusion:Differential thermoregulation between different genotypes of ATP1A1 gene indicate that the ATP1A1 gene could be potentially contributing to thermotolerance in both, Tharparkar, an indigenous breed and Vrindavani, a composite crossbred cattle.

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“…Heat st ress r esul t ed fr om h i gh a m bien t temperature and humidity has huge economic impact on the global dairy industry, and has adverse effects on health, production and reproduction in dairy animals. Heat stress also causes oxidative stress in dairy cows (Bernabucci et al, 2002) and influences the plasma electrolyte balance (Banerjee and Ashutosh, 2011) with a significant change in Sodium-Potassium ATPase enzyme (Na + , K + -ATPase) activity (Kashyap et al, 2014). This transmembrane ca rr i er en z yme i s r espon si ble for est abl i shi n g electrochemical gradient of Na + and K + ions across the plasma membrane and represents a plausible candidate for heat tolerance traits (Geering et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Single nucleotide polymorphisms in ATP1A1 gene and their association with thermotolerance traits in Sahiwal and Karan Fries cattle

Das

Gupta

Singh

et al. 2016

IJAR

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Expression of stress genes under thermal stress partially explain the relatively higher thermal adaptability of indigenous breeds compared to exotic breeds of cattle. ATP1A1 gene is one of such gene that encodes for a1 isomer of Na+, K+- ATPase enzyme for Na+- coupled transport of metabolites, nutrients, ions and represents a plausible candidate for heat tolerance traits. Present study was designed to compare SNP variations in ATP1A1 gene and to evaluate their association with respiration rate, rectal temperature and heat tolerance coefficient in Sahiwal (51) and Karan Fries (50) cows maintained at Livestock Research Centre of National Dairy Research Institute, Karnal. Two SNPs (T27008243C and A27008223G) were identified in both the dairy cattle breeds. Association of each SNP genotype was analyzed using Generalized Linear Model procedure in Statistical Analysis System (SAS). Sahiwal cows with TT (15.91±1.89b) at T27008243C locus had lowest RR compared to TC genotypes (18.25±1.77ab) and CC genotype (19.24±1.52a), while in Karan Fries cows RR for AA genotype at A27008223G locus was lower (28.85±1.96b) compared to GG genotype (32.37±2.51a). Thus, the study indicated that the TT genotype at T27008243C locus in Sahiwal and AA genotype at 27008223 locus in Karan Fries cows are desirable genotypes for genetic adaptability under heat stress.

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Influence of ambient temperature and humidity on ATP1A1 gene expression in Tharparkar and Vrindavani cattle

Cited by 8 publications

References 0 publications

Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle

Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle

Association of ATP1A1 gene polymorphism with thermotolerance in Tharparkar and Vrindavani cattle

Single nucleotide polymorphisms in ATP1A1 gene and their association with thermotolerance traits in Sahiwal and Karan Fries cattle

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