Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis

Abstract

In this work, direct and indirect methods for determination of vitamin K 3 were developed using differential pulse polarography. The reaction between sulfite and the quinone (Q) form of vitamin K 3 was used for indirect determination, since the sulfite peak at -0.7 V is sharp and very reproducible in 0.1 M HAc NaAc (pH = 5.5). It was found that at pH 4.5-5.5, this reaction was quantitative and very fast when the temperature was 45 • C. For its direct determination, on the other hand, vitamin K 3 was standardized by the indirect method using standard sulfite as the reducing agent. The calibration graph for vitamin K 3 (in Q form), using the peak at -1.0 V in a HAc-NaAc medium with a pH of 5.5, was linear for concentrations ranging from 5 × 10 −7 to 3 × 10 −5 M, and the limit of detection (LOD) was 1.5 × 10 −7 M. The proposed methods were successfully applied to the determination of vitamin K 3 in a clinical injection solution and in blood serum.

Effects of Melatonin on Oxidative Stress Index and Alveolar Bone Loss in Diabetic Rats With Periodontitis

“…1). The polymorphonuclear leukocytes of the connective tissue (CT) subjacent to the junctional epithelium were counted using stereology software 30 ## (Fig. 2A).…”