Influence of age and caloric restriction on liver glycolytic enzyme activities and metabolite concentrations in mice

Hagopian, Kevork; Ramsey, Jon J.; Weindruch, Richard

doi:10.1016/s0531-5565(02)00203-6

Cited by 76 publications

(87 citation statements)

References 51 publications

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“…Similar results were obtained in rats by Dhahbi et al (1999), who reported a significant decrease in PFK and PK mRNA in their underfed animals. These results are coherent with the ones obtained by Feuers et al (1989); Hagopian et al (2003) and Ugochukwu and Figgers (2006) concerning PK and PFK activities in fed restricted rats.…”

Section: Discussionsupporting

confidence: 92%

“…van found an increase in the GDH mRNA of restricted wild rabbits (2.28-fold) compared with the control group. Hagopian et al (2003) verified increased activity of this enzyme. In both experiments, the restriction level was much higher than in the present work (85% of daily maintenance requirements against 60% and 75%, respectively), which can justify protein catabolism due to the GDH increased genomic and activity levels.…”

Section: Lipolysismentioning

confidence: 59%

“…Several studies have been performed in order to characterize the effects of caloric restriction on gene expression of enzymes involved in energy metabolism (Dhahbi et al, 1999;Hagopian et al, 2003;van Harten and Cardoso, 2010). These studies showed differences in enzyme levels during periods of weight loss, suggesting that these changes can be maintained or increased in animals adapted to restrictive feeding conditions.…”

Section: Introductionmentioning

confidence: 99%

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Gene expression of regulatory enzymes involved in the intermediate metabolism of sheep subjected to feed restriction

Harten

Brito

Almeida

et al. 2013

Animal

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The effect of feed restriction on gene expression of regulatory enzymes of intermediary metabolism was studied in two sheep breeds (Australian Merino and Dorper) subjected to two nutritional treatments: feed restriction (85% of daily maintenance requirements) and control (ad libitum feeding), during 42 days. The experimental animals (ram lambs) were divided into four groups, n 5 5 (Australian Merino control (MC), Australian Merino Restriction (MR), Dorper control (DC) and Dorper Restriction (DR)). After the trial, animals were sacrificed and samples were taken from liver tissue to quantify glucose levels and gene expression of relevant intermediary metabolism enzymes (phosphofructokinase (PFK), pyruvate kinase (PK), phosphoenolpyruvate carboxykinase, fructose 1,6-bisphosphatase, glucose-6-phosphatase, glycogen synthase (GS), fatty acid synthase (FAS), glutamate dehydrogenase (GDH) and carbamoyl phosphate synthase (CPS)) through real-time PCR. During the experimental period, the MR animals lost 12.6% in BW compared with 5.3% lost by the Dorper lambs. MC and DC rams gained, respectively, 8.8% and 14% during the same period. Within the Dorper breed, restricted feed animals revealed a significant decrease over controls in the transcription of PFK (1.95-fold) and PK (2.26-fold), both glycolytic enzymes. The gluconeogenesis showed no change in the feed restricted animals of both breeds. DR feed group presented a significant decrease over the homologous Merino sheep group on GS. In both experimental breeds, FAS mRNA expression was decreased in restricted feed groups. GDH expression was decreased only in the DR animals (1.84-fold) indicating a reduced catabolism of amino acids in these animals. Finally, CPS was significantly (P , 0.05) higher in the Dorper sheep, indicating a facilitated urea synthesis in this breed. These results indicate a better adaptation of metabolic intermediate regulatory enzymes and hepatic glucose production of Dorper sheep to feed restriction concurring with the BW results in the experimental groups. ImplicationsThe relevant negative impact in livestock production due to seasonal dry periods within tropical and arid climate regions highlights the need to understand genetic and metabolic adaptation mechanisms to periodical feed restriction. Our results concerning changes in BW and genomic expressions of intermediary metabolism regulatory enzymes of ram lambs of Dorper and Australian Merino breeds subjected to ad libitum and to 85% maintenance energy requirements show that Dorper have a better adaptation to feed restriction than the Merino sheep. Our findings can contribute to future research guidelines leading to improved genetic selection toward livestock adaptation to harsh environments.

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Section: Discussionsupporting

confidence: 92%

Section: Lipolysismentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

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Gene expression of regulatory enzymes involved in the intermediate metabolism of sheep subjected to feed restriction

Harten

Brito

Almeida

et al. 2013

Animal

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“…genes and metabolic pathways regulating glycolysis [266][267][268]. This is important, as enhanced glycolysis is required for the rapid growth and survival of many tumor cells [21,22].…”

Section: Targeting Glucosementioning

confidence: 99%

“…Besides lowering circulating glucose levels, dietary energy restriction elevates circulating levels of fatty acids and ketone bodies (β-hydroxybutyrate and acetoacetate) [266,269,270]. Fats and especially ketone bodies can replace glucose as a primary metabolic fuel under calorie restriction.…”

Section: Targeting Glucosementioning

confidence: 99%

Cancer as a Metabolic Disease

Seyfried¹

2012

190

291

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Emerging evidence indicates that impaired cellular energy metabolism is the defining characteristic of nearly all cancers regardless of cellular or tissue origin. In contrast to normal cells, which derive most of their usable energy from oxidative phosphorylation, most cancer cells become heavily dependent on substrate level phosphorylation to meet energy demands. Evidence is reviewed supporting a general hypothesis that genomic instability and essentially all hallmarks of cancer, including aerobic glycolysis (Warburg effect), can be linked to impaired mitochondrial function and energy metabolism. A view of cancer as primarily a metabolic disease will impact approaches to cancer management and prevention.

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Metabolic Management of Cancer

Seyfried¹

2012

Cancer as a Metabolic Disease

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Influence of age and caloric restriction on liver glycolytic enzyme activities and metabolite concentrations in mice

Cited by 76 publications

References 51 publications

Gene expression of regulatory enzymes involved in the intermediate metabolism of sheep subjected to feed restriction

Gene expression of regulatory enzymes involved in the intermediate metabolism of sheep subjected to feed restriction

Cancer as a Metabolic Disease

Metabolic Management of Cancer

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