Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls

Abstract

Abstract:This study was conducted to explore the adding effect of manganese (Mn +2 ) to Tris extender on post cooling and cryopreservation semen attributes of Holstein bulls for different periods. Seven Holstein bulls of 3.5-4.5 years old were used in this study. Pooled semen was divided into three groups. The first group was diluted with Tris only (control group, G1). While the 2 nd (G2) and 3 rd (G3) groups were added 0.7 and 0.9 mM Mn +2 respectively. The effect of these additions on Holstein bulls semen quality was studied during different periods (cooling at 5 Celsius, 72 hrs.,1 st , 2 nd and 3 rd post cryopreservation, PC). Addition of Mn +2 led to significant increases of total ( ×10 6 ) plasma membrane integrity of sperms and total function sperm fraction as compared with control groups during cooling and PC periods. It can be concluded that the addition two levels of acid Mn +2 led to enhanced PC semen quality of bulls. This will in turn improved fertility rate of cows and owner's economic income.

Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C

“…The above could be attributed to high mitochondrial membrane potential maintained by sperm preserved with manganese. In a study by Eidan et al [ 37 ], the addition of Mn 2+ to a Tris extender significantly increased total counts (×10 6 ) of motile spermatozoa, improved acrosomal integrity and increased the counts of spermatozoa with normal morphology relative to control groups in different periods of the sperm production cycle. Manganese easily penetrates sperm cells, and maintains and restores the appropriate ion balance inside cells, thus minimizing the adverse effects of sperm preservation procedures [ 36 ].…”

Effect of Adding Different Levels of Nano-Zinc Oxide in Tris Diluent on some Traits of Chilled Semen of Bulls from the Artificial Insemination Center in Iraq

“…The results in Table (2) showed that there were highly significant differences (P<0.01) in the percentage of live sperm after cooling, as the treatment (T2), which recorded (75.20 ± 0.37), excelled on the treatments T1, T3, and T4, which recorded (71.80 ± 0.58). (62.00±1.22) and 56.00±(1.70, respectively.…”

“…The fertility of bulls is considered an important issue in artificial or natural insemination of cows, in addition to its economic importance within breeding systems [1,2] . In the process of artificial insemination, the fertility of bulls is linked to the ability of the bull to produce sperm and fertilize the egg, by increasing the number of progressively motile sperm and reducing the rate of deformities in the semen, which in turn leads to an increase in the pregnancy rate in cows [3,4] .…”

Some Fatty Acid and Semen Characteristics of Holstein Bulls as Inluenced by Different Sperm Freezabality

“…The cow pregnancy rate increases with the number of progressively motile sperm, an increase in straw for artificial insemination, and depending on the freezability of sperm (29). Sperm freezability per bull varies by semen components, diluent types, and additives (20,21,44). Definition of freezability: the ratio of individual sperm motility after cryopreservation to individual sperm motility in fresh sperm (21).…”

“…Mart´ınez-Soto et al (35) indicated the possibility of using seminal fatty acids to predict cryopreservation ability in men. Many studies have shown the improvement of the cryopreservation ability and viability of the Holstein bull's sperm by adding fatty acids or omega-3 to the extenders (20,25,26,27,28). Argov-Argaman et al (10), AL-Gebour found and Eidan (4,5) a change in the many fatty acids concentrations in seminal plasma and sperm between the winter and summer seasons.…”