Influence of Acute Exercise on the Osmotic Stability of the Human Erythrocyte Membrane

Paraíso, Lara Ferreira; Freitas, Mariana Vaini de; Gonçalves-e-Oliveira, Ana Flávia Mayrink; Neto, Omar Pereira de Almeida; Pereira, E. A. L.; Netto, Rangel Cerceau; Cunha, Lucas Moreira; Neto, Morun Bernardino; Agostini, Guilherme Gularte de; Resende, Elmiro Santos; Penha-Silva, Nilson

doi:10.1055/s-0034-1371834

Cited by 5 publications

(16 citation statements)

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“…The reduction of 1/H 50 after the acute training with the highest intensity among all (18th week) means that there was a decrease in osmotic stability after this training session. This finding is in agreement with the results found by Paraiso et al, who reported a significant reduction in the value of 1/H 50 when participants performed a high intensity training [ 17 ].…”

Section: Discussionsupporting

confidence: 93%

“…Oxidative stress is also an important factor associated with the reduction in membrane stability, since it is capable of destabilizing the plasma membrane [ 17 ], increasing its permeability [ 48 ] and reducing its fluidity [ 29 , 49 , 50 ]. It is possible that the decrease in the osmotic stability of erythrocytes observed in vitro after intense training in this study is directly related to oxidation processes promoted by this specific type of training, which would have destabilized in vivo the membrane of those cells.…”

Section: Discussionmentioning

confidence: 99%

“…The graphs of absorbance at 540 nm (A 540 ) as a function of the NaCl concentration (X) were fitted to sigmoidal regression lines ( Fig 1 ) according with the Boltzmann equation: where A min (absorbance units) and A max (absorbance units) represent respectively the mean values of A 540 at the minimum and maximum plateaus, H 50 (g/dL NaCl) is the NaCl concentration capable of promoting 50% hemolysis, and dX (g/dL NaCl) is the variation in the salt concentration that is responsible for the complete hemolysis transition. The parameters dX and 1/H 50 were used to evaluate the osmotic stability of the erythrocyte membrane, since they present same direction associations [ 17 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…The stability can be defined as the membrane's ability to resist lysis against many harmful agents. Many factors that affect the stability of the membrane are well known; among them are diet, age, temperature, use of drugs and some physiological and pathological conditions [ 13 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…The number of studies involving exercise and membrane stability is scarce [ 4 , 17 , 23 , 24 ]. Little is known about the role that exercise plays in the erythrocyte membrane.…”

Section: Introductionmentioning

confidence: 99%

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Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers

et al. 2017

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This study aimed to evaluate the influence of acute and chronic exercise on erythrocyte membrane stability and various blood indices in a population consisting of five national-level male swimmers, over 18 weeks of training. The evaluations were made at the beginning and end of the 1st, 7th, 13th and 18th weeks, when volume and training intensity have changed. The effects manifested at the beginning of those weeks were considered due to chronic adaptations, while the effects observed at the end of the weeks were considered due to acute manifestations of the exercise load of that week. Acute changes resulting from the exercise comprised increases in creatine kinase activity (CK) and leukocyte count (Leu), and decrease in hematocrit (Ht) and mean corpuscular volume (MCV), at the end of the first week; increase in the activities of CK and lactate dehydrogenase (LDH), in the uric acid (UA) concentration and Leu count, at the end of the seventh week; increases in CK and LDH activities and in the mean corpuscular hemoglobin concentration (MCHC), at the end of the 13th week; and decrease in the value of the osmotic stability index 1/H50 and increases in the CK activity and platelets (Plt) count, at the end of the 18th week. Chronic changes due to training comprised increase in the values of 1/H50, CK, LDH, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), serum iron (Fe), MCV and Plt. Although acute training has resulted in decrease in the osmotic stability of erythrocytes, possibly associated with exacerbation of the oxidative processes during intense exercise, chronic training over 18 weeks resulted in increased osmotic stability of erythrocytes, possibly by modulation in the membrane cholesterol content by low and high density lipoproteins.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The number of studies involving exercise and membrane stability is scarce [ 4 , 17 , 23 , 24 ]. Little is known about the role that exercise plays in the erythrocyte membrane.…”

Section: Introductionmentioning

confidence: 99%

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Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers

et al. 2017

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Influência do estado nutricional sobre a estabilidade osmótica de eritrócitos de idosos institucionalizados

Andrade¹

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Dedico este trabalho aos meus pais, Maria Leopoldina dos Santos (sempre presente) e Antônio Paulo Andrade, a quem me deram a vida, responsáveis pela minha educação onde imprimiram o amor pelos estudos e pela busca contínua do conhecimento, dedicação, cujo exemplo de vida familiar e profissional. v AGRADECIMENTOS A Deus, presença constante em minha vida, guiando, abençoando e iluminando todos os meus passos, provando que tudo tem seu tempo e sua hora e que basta confiar! Agradeço por tantas bênçãos e todos os planos, sempre tão perfeitos! À Carla Cristina Alves Andrade, minha esposa, minha amiga, companheira para todas as horas. Muito obrigado por todo seu amor, pela sua intensa dedicação, incentivo e apoio incondicional! Que Deus abençoe você e ilumine seu caminho sempre! Aos meus pais, Maria Leopoldina dos Santos e Antônio Paulo Andrade, meus grandes amigos e meu maior exemplo de humildade e dedicação. Muito obrigado pela vida e por sempre ter acreditado em mim. Mamãe infelizmente a senhora não está aqui no final de meu trabalho, mas sei que a senhora está muito feliz por eu ter chegado ao final de mais esta etapa! Agradeço à toda minha família, sobrinhos, tios, e em especial meus irmãos Antônio de Pádua dos Santos Andrade e Ana Paula dos Santos Andrade Viana, pelo apoio e pela compreensão do tempo de convívio muitas vezes sacrificado para realização deste trabalho. Ao Prof. Dr. Carlos Henrique Alves de Rezende, muito obrigado pela amizade, orientação e por todo seu apoio e incentivo, muito obrigado por ter acreditado em mim e por toda confiança depositada neste estudo. E parabéns pela sua admirável dedicação à vida acadêmica. Obrigado pelo apoio e incentivo nos momentos difíceis que passei durante o mestrado. Ao Prof. Dr. Nilson Penha-Silva, muito obrigado por ter sempre me acolhido em seu laboratório! Obrigado pelos ensinamentos, amizade e apoio! Muito obrigado pela oportunidade! Ao Dr. Paulo César Sousa, Diretor Geral da Faculdade Patos de Minas, pela valiosa colaboração na realização deste estudo, apoio e incentivo. Ao Me. Estanislau Jovtei Gonçalves Coordenador Acadêmico da Faculdade Patos de Minas por acreditar em meu trabalho, pelo apoio e amizade. Ao Dr. Mario da Silva Garrote Filho em especial pela amizade que encontrei durante esta jornada, pela parceria, pelo apoio, ensinamentos e pelo companheirismo! Muito obrigado por toda ajuda que tive de vocês e de todos da família LABFIQ. A todos os professores do PPGCS da FAMED-UFU, pelo convívio e por todo conhecimento compartilhado. vi Às secretárias do PPGCS da FAMED-UFU, Gisele e Viviane, por toda atenção, por toda ajuda e boa vontade de sempre. Aos funcionários do Laboratório de Análises Clínicas da Faculdade Patos de Minas, pelas análises e disponibilidade em ajudar. Aos voluntários que participaram deste estudo, pela grande generosidade e doação. Em especial à Faculdade Patos de Minas pela oportunidade, pelo financiamento deste estudo e por oportunizar a realização do mesmo.

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Relação entre estabilidade de membrana de eritrócitos, variáveis hematológicas, bioquímicas, função cognitiva e depressão em idosos institucionalizados

Andrade¹

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Introduction: Aging is a complex multifactorial process associated with numerous degenerative changes in structure and behavior of biological membranes. The erythrocyte membrane stability is defined as the membrane's ability to maintain its structure in adverse situations. Objective: This study aimed to evaluate the association between erythrocytes membrane stability, cognitive function and depression in institutionalized elderly. Methods: A population of 69 institutionalized elderly was investigated with regard to the osmotic stability of their erythrocyte membrane, erythrogram, lipid profile, cognition and depression. Results: Values of the hematological and biochemical variables are within their respective reference ranges. The existence of scores of GDS-15 suggestive of depression was much higher in females (83.7%) than in males (53.6%), which is a common trend in the epidemiology of depression among the elderly. The GDS scores were associated with increased erythrocyte membrane stability, platelet count and levels of total and LDL-cholesterol. The erythrocyte membrane stability also presented a positive relationship with the LDL-cholesterol levels and was not related to cognitive function. Conclusion: Depression in the elderly population of this study was associated with increases in the erythrocytes membrane stability and platelets counts, possibly because of the elevation in blood cholesterol levels. Moreover, the cognitive status was not significantly correlated with erythrocyte membrane stability.

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Influence of Acute Exercise on the Osmotic Stability of the Human Erythrocyte Membrane

Cited by 5 publications

References 15 publications

Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers

Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers

Influência do estado nutricional sobre a estabilidade osmótica de eritrócitos de idosos institucionalizados

Relação entre estabilidade de membrana de eritrócitos, variáveis hematológicas, bioquímicas, função cognitiva e depressão em idosos institucionalizados

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