Influence of a mouthwash containing hydroxyapatite microclusters on bacterial adherence in situ

Hannig, Christian; Basche, Sabine; Burghardt, T.; Al‐Ahmad, Ali; Hannig, Matthias

doi:10.1007/s00784-012-0781-6

Cited by 77 publications

(107 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The bioadhesion processes were determined in situ , considering that in vivo and in vitro conditions differ considerably [25]. The methodical approach, including bovine enamel and dentin specimens fixed to individual upper jaw splints, has already been used in previous studies [20, 25]. The overnight trial is an approved method to evaluate the impact of oral therapeutics of initial biofilm formation [25].…”

Section: Discussionmentioning

confidence: 99%

“…The LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen, Molecular probes, Darmstadt, Germany) adopts two stains—green fluorescent SYTO 9 stain (component A) and red-fluorescent propidium iodide stain (component B) [20]. The assays were performed according to manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…The epifluorescence assays were conducted at 1,000-fold magnification as in several previous studies (Axioskop II, ZEISS, Oberkochen, Germany) [20]. The number of cells observed in 10 randomized microscopic ocular grid fields per sample was counted.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of CPP/ACP on Initial Bioadhesion to Enamel and DentinIn Situ

Grychtol

Basche

Hannig

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

The present in situ study investigated the influence of a preparation containing CPP/ACP (caseinphosphopeptide-amorphous calcium phosphate) (GC Tooth mousse) on initial bacterial colonization of enamel and dentin. Therefore, pellicle formation was performed in situ on bovine enamel and dentin specimens fixed to individual upper jaw splints worn by 8 subjects. After 1 min of pellicle formation GC Tooth mousse was used according to manufacturer's recommendations. Rinses with chlorhexidine served as positive controls. Specimens carried without any rinse served as negative controls. After 8 h overnight exposure of the splints, bacterial colonization was quantified by fluorescence microscopy (DAPI and BacLight live/dead staining). Additionally, the colony forming units (CFU) were determined after desorption. Furthermore, the effects on Streptococcus mutans bacteria were tested in vitro (BacLight). There was no significant impact of CPP/ACP on initial bacterial colonization proved with DAPI and BacLight. Determination of CFU showed statistical significance for CPP/ACP to reduce bacterial adherence on enamel. The in vitro investigation indicated no antimicrobial effects for CPP/ACP on Streptococcus mutans suspension. Under the chosen conditions, CPP/ACP (GC Tooth mousse) had no significant impact on initial biofilm formation on dental hard tissues. The tested preparation cannot be recommended for biofilm management.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of CPP/ACP on Initial Bioadhesion to Enamel and DentinIn Situ

Grychtol

Basche

Hannig

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

show abstract

“…DAPI (4 0 , 6-diamidino-2-phenylindole) is a fluorescent chemical capable of forming the fluorescent complexes with double stranded DNA and yielding strong fluorescent signal [46][47][48][49] . The maximum of fluorescence was observed at a wavelength of 461 nm.…”

Section: Dapi Stainingmentioning

confidence: 99%

“…After steel samples were immersed in 800 ml tap water for 24,48 and 72 h, respectively, they were taken out and washed with PBS for 3 times, and then dried at room temperature. The samples were analysed by CLSM [49] .…”

Section: Live/dead Stainingmentioning

confidence: 99%

Antibacterial Performance of a Cu-bearing Stainless Steel against Microorganisms in Tap Water

et al. 2015

Journal of Materials Science & Technology

View full text Add to dashboard Cite

Date of Acceptance: 11/11/2014Tap water is one of the most commonly used water resources in our daily life. However, the increasing water contamination and the health risk caused by pathogenic bacteria, such as Staphylococcus aureus and Escherichia coli have attracted more attention. The mutualism of different pathogenic bacteria may diminish antibacterial effect of antibacterial agents. It was found that materials used for making pipe and tap played one of the most important roles in promoting bacterial growth. This paper is to report the performance of an innovative type 304 Cu-bearing stainless steel (304CuSS) against microbes in tap water. The investigation methodologies involved were means of heterotrophic plate count, contact angle measurements, scanning electron microscopy for observing the cell and subtract surface morphology, atomic absorption spectrometry for copper ions release study, and confocal laser scanning microscopy used for examining live/dead bacteria on normal 304 stainless steel and 304CuSS. It was found that the surface free energy varied after being immersed in tap water with polar component and Cu ions release. The results showed 304CuSS could effectively kill most of the planktonic bacteria (max 95.9% antibacterial rate), and consequently inhibit bacterial biofilms formation on the surface, contributing to the reduction of pathogenic risk to the surrounding environments

show abstract

Moderne Zahnpflege aus chemischer Sicht

Epple

Enax

2018

Chemie in nserer Zeit

View full text Add to dashboard Cite

Zusammenfassung Die Mundgesundheit spielt in unserer Gesellschaft eine zentrale Rolle. Deshalb gibt es eine Vielzahl von wirkungsvollen Zahnpflegepräparaten, die die Mundgesundheit verbessern und Zahn‐ und Zahnfleischerkrankungen vorbeugen können. Neben der Zahnbürste (Handzahnbürste, elektrische Zahnbürste) ist dabei insbesondere die Zahnpasta von Bedeutung. Als zentraler Wirkstoff wird Fluorid angesehen, wobei es auch interessante Entwicklungen von biomimetischen Ansätzen auf der Basis von Calciumphosphat gibt.

show abstract

Influence of a mouthwash containing hydroxyapatite microclusters on bacterial adherence in situ

Abstract: The tested mouthrinse seems to be a reasonable amendment for dental prophylaxis.

Cited by 77 publications

References 38 publications

Effect of CPP/ACP on Initial Bioadhesion to Enamel and DentinIn Situ

Effect of CPP/ACP on Initial Bioadhesion to Enamel and DentinIn Situ

Antibacterial Performance of a Cu-bearing Stainless Steel against Microorganisms in Tap Water

Moderne Zahnpflege aus chemischer Sicht

Contact Info

Product

Resources

About