Influence of a low background radiation environment on biochemical and biological responses in V79 cells

Satta, L.; Antonelli, Francesca; Belli, M.; Sapora, O.; Simone, G.; Sorrentino, E.; Tabocchini, M. A.; Amicarelli, Fernanda; Ara, Carmen; Cerù, Maria Paola; Colafarina, Sabrina; Devirgiliis, Laura Conti; Marco, Ario de; Balata, M.; Falgiani, Antonella; Nisi, S.

doi:10.1007/s00411-002-0159-2

Cited by 58 publications

(47 citation statements)

References 27 publications

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“…Thus even larger cells are still hit relatively rarely (on average, once every 25 days). In long duration experiments across both yeast and mammalian cells, evidence of a hormetic response to radiation has been observed in low background experiments [3, 7, 8, 64], where a small level of radiation seems to stimulate oxidative response mechanisms. Yet the mechanism by which information about very infrequent radiation energy deposition events is passed through the cell population or down the cell lineage remains to be understood.…”

Section: Discussionmentioning

confidence: 99%

Simulating the Impact of the Natural Radiation Background on Bacterial Systems: Implications for Very Low Radiation Biological Experiments

Lampe¹,

Biron²,

Brown³

et al. 2016

PLoS ONE

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At very low radiation dose rates, the effects of energy depositions in cells by ionizing radiation is best understood stochastically, as ionizing particles deposit energy along tracks separated by distances often much larger than the size of cells. We present a thorough analysis of the stochastic impact of the natural radiative background on cells, focusing our attention on E. coli grown as part of a long term evolution experiment in both underground and surface laboratories. The chance per day that a particle track interacts with a cell in the surface laboratory was found to be 6 × 10−5 day−1, 100 times less than the expected daily mutation rate for E. coli under our experimental conditions. In order for the chance cells are hit to approach the mutation rate, a gamma background dose rate of 20 μGy hr−1 is predicted to be required.

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Section: Discussionmentioning

confidence: 99%

Simulating the Impact of the Natural Radiation Background on Bacterial Systems: Implications for Very Low Radiation Biological Experiments

Lampe¹,

Biron²,

Brown³

et al. 2016

PLoS ONE

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“…We have exposed Chinese hamster V79 cells to repeated low doses of hydrogen peroxide (H 2 O 2 ) in order to characterize the effect of chronic low dose exposure in oxidants of mammalian cells. While the effect of repeated low dose exposure to radiations, like g rays and ultraviolet light, have been studied in different cellular system (Chatterjee et al, 1984;Osmak, 1988;Satta et al, 2002), no such studies were done for oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

Induced resistance in cells exposed to repeated low doses of HO involves enhanced activity of antioxidant enzymes

Bose

Bhaumik

Ghosh

2005

Cell Biology International

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We have derived cells from the Chinese hamster V79 cell line by conditioning them with repeated low doses of hydrogen peroxide (H(2)O(2)). This mimics the physiological condition where cells are repeatedly exposed to low levels of oxidants. In an attempt to characterize such cells, we have exposed both conditioned cells (V79(C)) and the parental V79 cells (V79(P)) to different types of cytotoxic agents and compared their sensitivity to cell killing. The V79(C) cells were found to be stably resistant to killing by agents that produced toxicity through oxidative stress, e.g. H(2)O(2) and cisplatin. It was also found that the lipid peroxidation produced by these agents were considerably lower in the V79(C) cells. Thus, the difference in sensitivity could be due to lesser extent of damage to these cells. V79(C) cells had greater antioxidant defense through higher GSH content and greater activity of enzymes such as Cu-Zn superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), which provided protection from damage. Enzyme activities were also assayed at different times after treatment with various cytotoxic agents; there was a relatively large increase in SOD activity which perhaps plays a key role in determining the resistance of the V79(C) cells to killing.

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“…Findings contrast with Satta et al who found a significant increase in cell density at confluence in V79 cells grown in the LNGS compared to parallel populations cultured above ground [8,17]. These disparate results may be explained by dissimilar methodology.…”

Section: Discussionmentioning

confidence: 60%

“…Other researchers found less dramatic effects, reporting reduced growth rates in paramecium, bacteria and some mammalian cells cultured in the deep underground environment and/or when shielded from cosmic radiation [5][6][7]. Some studies showed no apparent difference in growth rates in human lymphoblastoid TK6 cells and Chinese hamster V79 cells cultured in the Gran Sasso National Laboratory (LNGS) compared to a control environment [8,9]. The reasons for these contrasting results remain to be elucidated [3].…”

Section: Introductionmentioning

confidence: 99%

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Proteomics provides insights into the inhibition of Chinese hamster V79 cell proliferation in the deep underground environment

Liu

et al. 2020

Preprint

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Background: To characterize the environment of deep underground laboratory (DUGL) with a rock cover of 1470m and observe the effect of the DUGL environment on the growth and metabolism of Chinese hamster V79 cells. Results: Six environmental parameters in the DUGL and an above ground laboratory (AGL; control) were monitored. Compared to the AGL, O2 concentration was not significantly different, total γ ray dose rate was significantly lower (p=0.005), and relative humidity (p<0.001), air pressure (p<0.001), and concentration of CO2 and radon gas (p<0.001) were significantly higher in the DUGL. The growth curves of cultured V79 cells showed cell proliferation was slower in the DUGL. Tandem mass tag (TMT) proteomics analysis was performed to identify differentially abundant proteins (DAPs) in V79 cells cultured in the DUGL and AGL. Parallel Reaction Monitoring (PRM) was conducted to verify TMT results. TMT detected 980 DAPs, defined as proteins with a ≥1.2-absolute fold change in relative abundance (p <0.05) between V79 cells cultured in the DUGL and AGL. Of these, 576 proteins were up-regulated and 404 proteins were down-regulated in V79 cells cultured in the DUGL. GO term enrichment analysis of up-regulated proteins revealed enrichment of proteins involved in translation, ribosome, proton-transporting ATP synthase activity, oxygen binding, and oxygen transporter activity et al. GO term enrichment analysis of downregulated proteins demonstrated enrichment of proteins involved in the endoplasmic reticulum lumenand respiratory chain. KEGG pathway analysis revealed that ribosome (p<0.001), base excision repair (p<0.001), RNA transport (p=0.009), Huntington's disease (p=0.023), and oxidative phosphorylation (OXPPL) (p=0.035) pathways were significantly enriched. Conclusion: Proliferation of V79 cells was inhibited in the DUGL, likely because cells were exposed to reduced cosmic ray muons flux. There were apparent changes in the proteome profile of the V79 cells cultured in the DUGL, which affected proteins related to the ribosome, RNA transport, translation, energy metabolism, and DNA repair.These proteins may have induced cellular changes that delayed proliferation but enhanced survival, making the V79 cells adaptable to the changing environment. Our findings provide insight into the cellular stress response that is triggered in the absence of normal levels of radiation.

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Influence of a low background radiation environment on biochemical and biological responses in V79 cells

Cited by 58 publications

References 27 publications

Simulating the Impact of the Natural Radiation Background on Bacterial Systems: Implications for Very Low Radiation Biological Experiments

Simulating the Impact of the Natural Radiation Background on Bacterial Systems: Implications for Very Low Radiation Biological Experiments

Induced resistance in cells exposed to repeated low doses of HO involves enhanced activity of antioxidant enzymes

Proteomics provides insights into the inhibition of Chinese hamster V79 cell proliferation in the deep underground environment

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