Influence of 24-Epibrassinolide on Water Exchange of Wheat Varieties Various in Dry Resistance under Osmotic Stress

Безрукова, М. В.; Kudoyarova, G. R.; Lubyanova, A. R.; Maslennikova, Dilara; Шакирова, Ф. М.

doi:10.1134/s1021443721010040

Cited by 5 publications

(1 citation statement)

References 24 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fresh weight was determined immediately after separating the leaves and roots from the plant. To determine turgor weight, shoots were placed at room temperature for 24 h in the dark in closed vessels, loading the base into distilled water [ 62 ], while roots were completely immersed in water for the same period [ 63 ]. Then, samples were dried in open containers, which provided free circulation of air, in a hot air oven at 70 °C until a constant dry mass was achieved.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Seed Pretreatment with Endophytic Bacteria Bacillus subtilis on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit

Lubyanova

Allagulova

Lastochkina

2023

Plants

View full text Add to dashboard Cite

We investigated the effect of pre-sowing seed treatment with endophytic Bacillus subtilis 10-4 (B. subtilis) on spring and winter wheat (Triticum aestivum L.; cultivars Ekada-70 (Ek) and Scepter (Sc), respectively) growth and tolerance under 1–24 h of drought stress, modulated by 12% polyethylene glycol 6000 (PEG). The results showed that drought decreased transpiration intensity (TI), root relative water content (RWC), osmotic potential (Ψπ) of cell sap, and induced proline accumulation and electrolyte leakage (EL) in both wheat cultivars. It was revealed that Sc was more responsive to PEG and B. subtilis treatments than Ek. Under drought, Ek did not significantly change root length, shoot height, or dry biomass. The pretreatment of wheat plants with B. subtilis performed significantly better under drought conditions through the enhanced TI, RWC, and Ψπ of the cell sap in comparison with the plants treated with 12% PEG alone. B. subtilis also reduced stress-caused EL, especially in the Sc cultivar. Under water deficit wheat seedlings, pretreated with B. subtilis, have a higher proline accumulation in comparison to untreated stressed plants. Taken together, our results demonstrate the crucial role of endophytic B. subtilis in ameliorating the adverse effects of water stress on the water balance of both winter and spring wheat cultivars.

show abstract

Section: Methodsmentioning

confidence: 99%

The Effects of Seed Pretreatment with Endophytic Bacteria Bacillus subtilis on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit

Lubyanova

Allagulova

Lastochkina

2023

Plants

View full text Add to dashboard Cite

show abstract

ROS-Dependent Stress-Protective Effect of 24-Epicastasterone and Its Monosalicylate on Wheat Seedlings in Hyperthermia

Litvinovskaya

Shkliarevskyi

Kolupaev

et al. 2021

Appl Biochem Microbiol

View full text Add to dashboard Cite

The indicator of cell membrane permeability of wheat seedlings in assessing stress resistance of wheat varieties

Гурова

Svezhintseva

Chesnochenko

2021

Sib. vestn. s-h. nauki

View full text Add to dashboard Cite

The paper presents the results of studies on the use of the permeability index of cell membranes, determined by the relative change in the specific electrical conductivity (EC) of water extracts of tissues of spring wheat seedlings, varieties Novosibirskaya 18, Novosibirskaya 44, Sibirskaya 21 and Omskaya 18, under the combined action of stressors. In model laboratory vegetation experiments, the hourly dynamics of the EC of water extracts of seedling leaves (exposure of leaves to water for 0.5-4.5 h) was investigated under the simultaneous action of chloride salinity (1.3%) and the causative agent of common rot of cereals Bipolaris sorokiniana Shoem. (5000 conidia per grain). It was established that EC increased by 1.5 times and the rate of electrolyte leakage increased twofold in the less resistant variety Novosibirskaya 44 compared to the more resistant Omskaya 18. The daily dynamics of the EC of 10-16-day-old seedlings was studied under the sequential action of seed hyperthermia (43 °C), chloride salinity (1.3%), and Bipolaris sorokiniana Shoem. (5000 conidia per grain). The protective effect of hyperthermia was established in the more resistant variety Sibirskaya 21 (a decrease in EC up to 1.3 times) in comparison with the variant without heating the seeds. In the less resistant variety Novosibirskaya 18, heating the seeds destabilized the state of the cell membranes (increase in EC and electrolyte leakage rate by 1.5 and 1.2 times respectively). The conditions that ensure the identification of the maximum differences in the studied wheat varieties were experimentally determined: the age of seedlings 10 days, the time interval of exposure of the samples to water 1.5-4.5 h. Intervarietal differences in the relative change in the EC values in the variant without heating the seeds were 1.9 times and in the variant with heating the seeds - 3.7 times, with the significance of difference at the levels p ≤ 0.05 and p ≤ 0.01. Intervarietal differences in the relative change in the EC, established for the time interval exposition of electrolytes leakage of 1.5-4.5 h, were 1.50-1.67 times with the significance of difference at the level of p ≤ 0.05. The proposed approach will make it possible to develop a methodology for assessing new genotypes for resistance to the combined action of biotic and abiotic stressors.

show abstract

Influence of 24-Epibrassinolide on Water Exchange of Wheat Varieties Various in Dry Resistance under Osmotic Stress

Cited by 5 publications

References 24 publications

The Effects of Seed Pretreatment with Endophytic Bacteria Bacillus subtilis on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit

The Effects of Seed Pretreatment with Endophytic Bacteria Bacillus subtilis on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit

ROS-Dependent Stress-Protective Effect of 24-Epicastasterone and Its Monosalicylate on Wheat Seedlings in Hyperthermia

The indicator of cell membrane permeability of wheat seedlings in assessing stress resistance of wheat varieties

Contact Info

Product

Resources

About