Influence de différentes concentrations d'acide indole-3-acétique, d'acide naphtalène-acétique, d'acide 2,4-dichlorophénoxy-acétique et de kinétine sur la callogenèse et l'organogenèse in vitro de fragments d'entre-nœuds de deux variétés de patate douce (Ipomoea batatas L. Lam.)

Abstract

(2,4-D) and kinetin on the callusing and the organogenesis in vitro of internode fragments of two varieties of sweet potato (Ipomoea batatas L. Lam.). The object of the study was the influence of indole 3 acetic acid (IAA), naphtalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin, applied separately or in association, on the callusing and organogenesis in vitro of internode fragments of 2, "white" and 'pink' ; varieties of sweet potato in relation to the polarity of explants and the varietal character.The different callusing and organogenesis potentialities of the explants have been analysed. The neoformation of roots and buds growing into plants has been obtained.The in vitro tuberization of the neoformed roots has been also obtained. It exclusively occurs in the presence of kinetin and auxin.The agronomic interest of these results for the practice of the vegetative propagation of sweet potato is underlined.Growth regulator -Ipomoea batatas -polarity -callusing -organogenesis -tuberization

Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin

“…In Benin, in vitro culture techniques are already successfully used for sanitation, micropropagation and ex situ conservation of certain root and tuber crops including yams (Ahanhanzo et al, 2010;Agbidinoukoun et al, 2013) and cassava (Cacaï et al, 2013). However, since the work of Houndonougbo (1989) on the influence of different concentrations of indole-3-acetic acid, naphthalene acetic acid, 2,4-dichlorophenoxyacetic acid and kinetin on callogenesis and in vitro organogenesis of inter-node fragments of two sweet potatoes landraces, no work has been reported on the in vitro culture of sweet potato in Benin as part of the improvement of the seed system. For the establishment of in vitro culture, bacteria and fungi are a cause of contamination of explants.…”