INFLORESCENCE DEFICIENT IN ABSCISSION Controls Floral Organ Abscission in Arabidopsis and Identifies a Novel Family of Putative Ligands in Plants

Butenko, Melinka A.

doi:10.1105/tpc.014365

Cited by 337 publications

(503 citation statements)

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“…Since NEV, IDA, HAE, and HSL2 each regulates the cell separation stage of organ separation (Butenko et al, 2003;Cho et al, 2008;Stenvik et al, 2008;Liljegren et al, 2009), we tested whether disruption of CST activity might also suppress the ida and hae hsl2 mutant phenotypes. We found that mutations in CST do not rescue the shedding defects of ida or hae hsl2 flowers (Fig.…”

Section: Organ Separation Is Restored In Nev Cst Flowersmentioning

confidence: 99%

“…The HAESA (HAE) and HAESA-LIKE2 (HSL2) LRR-RLKs redundantly activate a mitogen-activated protein kinase signaling cascade that leads to cell separation and release of the outer floral organs (Jinn et al, 2000;Cho et al, 2008). The predicted signaling ligand for HAE/ HSL2 is INFLORESCENCE DEFICIENT IN ABSCIS-SION (IDA), a small, secreted peptide (Butenko et al, 2003;Cho et al, 2008;Stenvik et al, 2008). Two inhibitors of organ separation that may directly regulate HAE/HSL2 are the EVERSHED (EVR) and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) LRR-RLKs Lewis et al, 2010).…”

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CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

et al. 2011

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Receptor-like kinase-mediated cell signaling pathways play fundamental roles in many aspects of plant growth and development. A pair of Arabidopsis (Arabidopsis thaliana) leucine-rich repeat receptor-like kinases (LRR-RLKs), HAESA (HAE) and HAESA-LIKE2 (HSL2), have been shown to activate the cell separation process that leads to organ abscission. Another pair of LRR-RLKs, EVERSHED (EVR) and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1, act as inhibitors of abscission, potentially by modulating HAE/HSL2 activity. Cycling of these RLKs to and from the cell surface may be regulated by NEVERSHED (NEV), a membrane trafficking regulator that is essential for organ abscission. We report here the characterization of CAST AWAY (CST), a receptor-like cytoplasmic kinase that acts as a spatial inhibitor of cell separation. Disruption of CST suppresses the abscission defects of nev mutant flowers and restores the discrete identity of the trans-Golgi network in nev abscission zones. After organ shedding, enlarged abscission zones with obscured boundaries are found in nev cst flowers. We show that CST is a dual-specificity kinase in vitro and that myristoylation at its amino terminus promotes association with the plasma membrane. Using the bimolecular fluorescence complementation assay, we have detected interactions of CST with HAE and EVR at the plasma membrane of Arabidopsis protoplasts and hypothesize that CST negatively regulates cell separation signaling directly and indirectly. A model integrating the potential roles of receptor-like kinase signaling and membrane trafficking during organ separation is presented.

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Section: Organ Separation Is Restored In Nev Cst Flowersmentioning

confidence: 99%

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confidence: 99%

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

et al. 2011

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“…IDA has been shown to control floral organ cell separation (abscission), [10][11][12] given that specialized abscission zone (AZ) cells are present. 13 IDA and the five Arabidopsis IDL proteins have an N-terminal signal peptide (SP) that has been shown to direct GFP-fusion proteins of IDA, IDL1 and IDL3 to the periphery of onion cells.…”

Section: Ida and The Idl Proteins Are Signaling Moleculesmentioning

confidence: 99%

“…13 IDA and the five Arabidopsis IDL proteins have an N-terminal signal peptide (SP) that has been shown to direct GFP-fusion proteins of IDA, IDL1 and IDL3 to the periphery of onion cells. 10,14 Their small size and extra-cellular localization made us suggest that these proteins are ligands involved in cell-tocell communication. 10 The SPs of IDA and the IDLs should direct the proteins to the apoplastic space, by the default pathway for soluble plant proteins, 15 and enable a potential receptor interaction.…”

Section: Ida and The Idl Proteins Are Signaling Moleculesmentioning

confidence: 99%

“…10,14 Their small size and extra-cellular localization made us suggest that these proteins are ligands involved in cell-tocell communication. 10 The SPs of IDA and the IDLs should direct the proteins to the apoplastic space, by the default pathway for soluble plant proteins, 15 and enable a potential receptor interaction. In agreement with this, we show that plants harboring constructs designed to overexpress IDA or IDL1 without the SP displayed none of the phenotypes observed in 35S:IDA and 35S:IDL1 plants.…”

Section: Ida and The Idl Proteins Are Signaling Moleculesmentioning

confidence: 99%

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Identification of a putative receptor-ligand pair controlling cell separation in plants

Stenvik

Butenko

Aalen

2008

Plant Signaling & Behavior

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receptor-ligand interaction on the cell surface. 4 The cell wall provides plants with strength and rigidity but also constrains the activity and autonomy of individual cells. [5][6][7][8] Most cells remain attached to their neighbors throughout their life, but during a number of processes in the life cycle of a plant (e.g., germination, formation of stomata, organ and seed shedding) it is crucial that separation between neighboring cells takes place. 9 IDA and the IDL Proteins are Signaling MoleculesIDA has been shown to control floral organ cell separation (abscission), [10][11][12] given that specialized abscission zone (AZ) cells are present. 13 IDA and the five Arabidopsis IDL proteins have an N-terminal signal peptide (SP) that has been shown to direct GFP-fusion proteins of IDA, IDL1 and IDL3 to the periphery of onion cells. 10,14 Their small size and extra-cellular localization made us suggest that these proteins are ligands involved in cell-tocell communication. 10 The SPs of IDA and the IDLs should direct the proteins to the apoplastic space, by the default pathway for soluble plant proteins, 15 and enable a potential receptor interaction. In agreement with this, we show that plants harboring constructs designed to overexpress IDA or IDL1 without the SP displayed none of the phenotypes observed in 35S:IDA and 35S:IDL1 plants. Receptor-ligand InteractionWe have previously proposed that IDA could be the ligand of the leucine-rich repeat (LRR)-RLK HAESA (HAE). 10,16 In our recent paper, we report that the double mutant of HAE and HAESA-LIKE2 (HSL2) has a block in floral abscission, a phenotype similar to that of ida. We also demonstrate that hae hsl2 is epistatic to 35S:IDA, providing genetic evidence that IDA, HAE and HSL2 act in the same pathway. We hypothesize that IDA can signal through both HAE and HSL2, but we can not say which of the two, or if both, receptors normally relay the IDA signal.When ectopically expressed all the IDL proteins were capable of inducing floral organ abscission. These results indicate that the IDL proteins are able to trigger abscission through the same cellular mechanism as IDA, and that the IDL proteins may function through similar signaling pathways. However, promoter-reporter gene constructs indicate that the IDL genes are expressed in diverse tissues and not only in the floral organ AZs, suggesting that their normal functions differ from that of IDA. Therefore it is probable that the putative IDL ligands can exert their effects both through an

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Abscission

Roberts¹,

Gonzalez‐Carranza²

2007

Encyclopedia of Life Sciences

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The abscission of organs takes place at discrete sites and at specific times during the life cycle of a plant. These observations suggest that the abscission zone comprises a layer of positionally differentiated cells that undergo separation from their neighbours in response to precise developmental and environmental cues. By studying mutants of Arabidopsis that exhibit an attenuated capacity to shed their floral organs, it has been possible to identify a number of genes that may play a role in both the differentiation of abscission zone cells and the timing of organ shedding. The possible applications of this knowledge to agricultural and horticultural species are discussed.

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INFLORESCENCE DEFICIENT IN ABSCISSION Controls Floral Organ Abscission in Arabidopsis and Identifies a Novel Family of Putative Ligands in Plants

Abstract: Abscission is an active process that enables plants to shed unwanted organs. Because the purpose of the flower is to facilitate pollination, it often is abscised after fertilization. We have identified an Arabidopsis ethylene-sensitive mutant, inflorescence deficient in abscission (

Cited by 337 publications

References 40 publications

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

Identification of a putative receptor-ligand pair controlling cell separation in plants

Abscission

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