Infliximab-based self-healing hydrogel composite scaffold enhances stem cell survival, engraftment, and function in rheumatoid arthritis treatment

Zhao, Yüe; Gao, Chaohua; Hou, Lei; Liu, Hangrui; Feng, Yubin; Li, Zuhao; Li, He; Wang, Jincheng; Yang, Bai; Lin, Quan

doi:10.1016/j.actbio.2020.12.005

Cited by 36 publications

(23 citation statements)

References 56 publications

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“…The presented results act as evidence that the viability, proliferation, and osteogenic differentiation capacity of encapsulated ADMSCs were maintained even under RA conditions. Down-regulation of inflammatory cytokines, induced osteogenesis, cartilage rebuilt, and improved bone repair detected in rabbit model after 3 months confirm the potential of employing antirheumatic drugs to construct hydrogels for stem cell-based therapies of RA [ 44 ].…”

Section: Resultsmentioning

confidence: 90%

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Implication of Mesenchymal Stem Cells and Their Derivates for Osteochondral Regeneration

Smolinská

Debreova

Culenova

et al. 2022

IJMS

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Healing of articular cartilage defects presents a challenging issue, due to its regenerative shortcomings. Lacking vascularity and innervation of cartilage and low proliferative potential of chondrocytes are the main reasons for the limited healing potential of articular cartilage. Traditional reparative approaches are limited in their efficiency, hence there is a demand for novel reparative treatments. Mesenchymal stromal cells, preferred for clinical uses, can be readily derived from various sources and have been proven to have a therapeutic effect on cartilage and subchondral bone. Therefore, mesenchymal stromal cells, their derivates, and scaffolds have been utilized in research targeting osteochondral regeneration. The present review aims to comprehensively outline and discuss literature considering this topic published within last 5 years.

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Section: Resultsmentioning

confidence: 90%

“…Hydrogels have attracted considerable interest from Zhao et al [ 44 ] and encouraged them to incorporate therapeutic antibodies, specifically infliximab, into hydrogel which was then used to encapsulate ADMSCs. They fabricated infliximab-based, self-healing hydrogel for regulating the hostile microenvironment of the RA site.…”

Section: Resultsmentioning

confidence: 99%

Implication of Mesenchymal Stem Cells and Their Derivates for Osteochondral Regeneration

Smolinská

Debreova

Culenova

et al. 2022

IJMS

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show abstract

“…The progressive damage of articular bone and cartilage was developed in RA patients, which might cause disability over time [ 40 ]. Recent evidence has suggested that bioengineered composite scaffolds and magnetic nanoparticles are effective promising therapeutic tools for RA remedy [ 41 , 42 ]. Therefore, the combination of nanotechnology and molecular targeted drugs might be a most effective method for RA treatment.…”

Section: Discussionmentioning

confidence: 99%

CircMAPK9 promotes the progression of fibroblast-like synoviocytes in rheumatoid arthritis via the miR-140-3p/PPM1A axis

Luo

Chen

2021

J Orthop Surg Res

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Background Rheumatoid arthritis (RA) is a chronic inflammatory joint disease, and fibroblast-like synoviocytes (FLSs) are key effector cells in RA development. Mounting evidence indicates that circular RNAs (circRNAs) participate in the occurrence and development of RA. However, the precise mechanism of circRNA mitogen-activated protein kinase (circMAPK9) in the cell processes of FLSs has not been reported. Methods The expression levels of circMAPK9, microRNA-140-3p (miR-140-3p), and protein phosphatase magnesium-dependent 1A (PPM1A) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot assay. Cell proliferation was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell apoptosis and cycle distribution were assessed by flow cytometry. Cell migration and invasion were tested by transwell assay. All the proteins were inspected by western blot assay. Inflammatory response was evaluated by enzyme-linked immunosorbent assay (ELISA). The interaction between miR-140-3p and circMAPK9 or PPM1A was verified by dual-luciferase reporter assay. Results CircMAPK9 and PPM1A were upregulated and miR-140-3p was downregulated in RA patients and FLSs from RA patients (RA-FLSs). CircMAPK9 silence suppressed cell proliferation, migration, invasion, inflammatory response, and promoted apoptosis in RA-FLSs. MiR-140-3p was a target of circMAPK9, and miR-140-3p downregulation attenuated the effects of circMAPK9 knockdown on cell progression and inflammatory response in RA-FLSs. PPM1A was targeted by miR-140-3p, and circMAPK9 could regulate PPM1A expression by sponging miR-140-3p. Furthermore, miR-140-3p could impede cell biological behaviors in RA-FLSs via targeting PPM1A. Conclusion CircMAPK9 knockdown might inhibit cell proliferation, migration, invasion, inflammatory response, and facilitate apoptosis in RA-FLSs via regulating miR-140-3p/PPM1A axis, offering a new mechanism for the comprehension of RA development and a new insight into the potential application of circMAPK9 in RA treatment.

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“…Several investigators have used MSC therapy in combination with treatments used in RA such as TNF-α inhibitors [ 66 , 78 ], IL-4 [79] or hesperidin [80] in preclinical models of RA aiming to increase the beneficial effects of MSC-based therapy. These groups demonstrated that the combination of MSCs with these molecules was more efficient than either of the treatments alone.…”

Section: Approaches For the Improvement Of Msc-based Therapy In Ra Experimental Modelsmentioning

confidence: 99%

Mesenchymal stem/stromal cell-based therapy for the treatment of rheumatoid arthritis: An update on preclinical studies

2021

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Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterized by synovial inflammation and progressive joint destruction and is a primary cause of disability worldwide. Despite the existence of numerous anti-rheumatic drugs, a significant number of patients with RA do not respond or are intolerant to current treatments. Mesenchymal stem/stromal cell (MSCs) therapy represents a promising therapeutic tool to treat RA, mainly attributable to the immunomodulatory effects of these cells. This review comprises a comprehensive analysis of the scientific literature related to preclinical studies of MSC-based therapy in RA to analyse key aspects of current protocols as well as novel approaches which aim to improve the efficacy of MSC-based therapy.

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Infliximab-based self-healing hydrogel composite scaffold enhances stem cell survival, engraftment, and function in rheumatoid arthritis treatment

Cited by 36 publications

References 56 publications

Implication of Mesenchymal Stem Cells and Their Derivates for Osteochondral Regeneration

Implication of Mesenchymal Stem Cells and Their Derivates for Osteochondral Regeneration

CircMAPK9 promotes the progression of fibroblast-like synoviocytes in rheumatoid arthritis via the miR-140-3p/PPM1A axis

Mesenchymal stem/stromal cell-based therapy for the treatment of rheumatoid arthritis: An update on preclinical studies

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