Inflicting Controlled Nonthermal Damage to Subcellular Structures by Laser-Activated Gold Nanoparticles

Krpetić, Željka; Nativo, Paola; Prior, Ian A.; Völk, Martin

doi:10.1021/nl103142t

Cited by 102 publications

(112 citation statements)

References 34 publications

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“…It allows to visually distinguish if the nanoparticles are located freely in the cytosol, or sequestered in membranous vesicles. For inorganic nanoparticles like Qdots [54], gold nanoparticles [107] and superparamagnetic iron oxide NP's [14,108], labeling steps are usually not necessary ( Figure 7A and C). However, organic nanoparticles cannot always be easily distinguished from the cellular structures, though for polymeric nanoparticles, opinions vary.…”

Section: Electron Microscopymentioning

confidence: 99%

Intracellular delivery of nanomaterials: How to catch endosomal escape in the act

et al. 2014

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Successful cytosolic delivery of nanomaterials is becoming more and more important, given the increase in intracellular applications of quantum dots, gold nanoparticles, liposomal drug formulations and polymeric gene delivery vectors. Most nanomaterials are taken up by the cell via endocytosis, yet endosomal escape has long been recognized as a major bottleneck in cytosolic delivery. Although it is essential to detect and reliably quantify endosomal escape, no consensus has been reached so far on the methods to do so. This review will summarize and discuss for the first time the different assays used to investigate this elusive step to date.

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Section: Electron Microscopymentioning

confidence: 99%

Intracellular delivery of nanomaterials: How to catch endosomal escape in the act

et al. 2014

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“…Of special relevance is ionization of water molecules and the generation of ROS [73,136,137]. ROS and free radicals can initiate a damaging chain reaction of lipid peroxidation followed by decreased hydrophobicity of the lipid bilayer [74,86].…”

Section: Photochemical-induced Photoporationmentioning

confidence: 99%

Laser-assisted photoporation: fundamentals, technological advances and applications

Xiong

Samal

Demeester

et al. 2016

Advances in Physics: X

104

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Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-ofthe-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticlemediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.

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“…The complex with PEI demonstrated an improved phototoxicity [124]. Gold nanoparticles have been used in a study to induce endosome rupture by low-intensity laser and release of the gold nanoparticles into the cytosol [125].…”

Section: Physicochemical Techniquesmentioning

confidence: 99%

Diving through Membranes: Molecular Cunning to Enforce the Endosomal Escape of Antibody-Targeted Anti-Tumor Toxins

Fuchs

Bachran²,

Flavell

et al. 2013

Antibodies

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Membranes are vital barriers by which cells control the flux of molecules and energy between their exterior and interior and also between their various intracellular compartments. While numerous transport systems exist for ions and small molecules, the cytosolic uptake of larger biological molecules and in particular antibody-targeted drugs, is a big challenge. Inducing leakage of the plasma membrane is unfavorable since the target cell specificity mediated by the antibody would likely be lost in this case. After binding and internalization, the antibody drug conjugates reach the endosomes. Thus, enforcing the endosomal escape of anti-tumor toxins without affecting the integrity of other cellular membranes is of paramount importance. Different strategies have been developed in the last decades to overcome endosomal accumulation and subsequent lysosomal degradation of targeted protein-based drugs. In this review we summarize the various efforts made to establish efficient techniques to disrupt the endosomal membrane barrier including the use of molecular ferries such as cell penetrating peptides or viral membrane fusion proteins, endosomal leakage inducing molecules such as saponins or monensin and physicochemical methods as represented by photochemical internalization.

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Inflicting Controlled Nonthermal Damage to Subcellular Structures by Laser-Activated Gold Nanoparticles

Cited by 102 publications

References 34 publications

Intracellular delivery of nanomaterials: How to catch endosomal escape in the act

Intracellular delivery of nanomaterials: How to catch endosomal escape in the act

Laser-assisted photoporation: fundamentals, technological advances and applications

Diving through Membranes: Molecular Cunning to Enforce the Endosomal Escape of Antibody-Targeted Anti-Tumor Toxins

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