Inflammatory response markers in rats undergoing abdominal surgical procedures

Kırdak, Türkay; Uysal, Erdal; Sezgın, Efe; Çeçen, Gülce Sevdar; Çavun, Sinan

doi:10.20524/aog.2020.0511

Cited by 6 publications

(14 citation statements)

References 43 publications

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“…Postoperative detection of WBCs has been demonstrated to be an indicator that predicts complications, such as infection or anastomotic leakage. 39 Theoretically, it is supposed to be conducive to evaluation of an inflammatory reaction following sleeve lobectomy. In the present study, the RAS group had lower WBC levels after operation compared to those of the MT group.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Sleeve Lobectomy for Lung Cancer Using Mini-Thoracotomy and an Optimized Robot-Assisted Technique

Tao

Yuan

Kang

et al. 2021

Technol Cancer Res Treat

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Objective: To evaluate the clinical significance of an optimized approach to improve surgical field visualization and simplify anastomosis techniques using robotic-assisted sleeve lobectomy for lung or bronchial carcinoma. Method: A total of 26 consecutive patients who underwent sleeve lobectomy between January 2017 and April 2020 were enrolled in the study. The cohort included 11 cases of robotic-assisted surgery (RAS group) and 15 cases of mini-thoracotomy (MT group). RAS was performed via an exclusive optimized approach utilizing the “3 to 4-6 to 8/9” four-port technique. Retrieved demographical and clinical data included operation time, anastomosis time, blood loss, chest drainage time and volume, postoperative pain scores, complications, white blood cell (WBC) levels, and duration of hospital stay and follow-up. Results: No cases of perioperative death were recorded. Compared to MT group, the RAS group had a similar anastomosis time (30.82 ± 6.08 vs 33.20 ± 7.73 min, respectively, p > 0.05) and shorter operation time (189.73 ± 36.41 vs 225.33 ± 38.19 min, respectively, p < 0.05). The RAS group had lower pain scores (4.23 ± 0.26 vs 4.91 ± 0.51, p < 0.05), lower levels of WBC (p < 0.05), and no anastomotic complications postoperatively. The RAS and MT groups demonstrated a successful bronchus reconstruction with low risk of angulation (1/11 vs 1/15, p > 0.05) and satisfactory disease-free survival (eight cases, 72.73% and 12 cases, 80%, respectively). Conclusion: The optimized approach to RA sleeve lobectomy is convenient and efficient and provides satisfactory clinical outcomes. Further study with a large sample size and evaluation of long-term survival are warranted. Key points: (i) we present a novel, convenient, and efficient approach for robotic-assisted sleeve lobectomy, ie, “3 to 4-6 to 8/9” four-port technique. The optimized approach for RA sleeve lobectomy is convenient and efficient and provides satisfactory clinical outcomes; (ii) details for the “3 to 4-6 to 8/9” four-port method: the assistant port was located at the fourth intercostal space. The 1-cm camera port was inserted at the sixth intercostal space in the posterior axillary line. The 0.5-cm da Vinci ports of the instrument arms were placed at the third intercostal space in the anterior axillary line and the eighth or ninth intercostal space in the posterior axillary line. The patient cart was inserted from the back of the patient's head and shoulders at 75° to the longitudinal line.

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Section: Discussionmentioning

confidence: 99%

Comparison of Sleeve Lobectomy for Lung Cancer Using Mini-Thoracotomy and an Optimized Robot-Assisted Technique

Tao

Yuan

Kang

et al. 2021

Technol Cancer Res Treat

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“…CRP has been studied in models of various abdominal surgeries (hepatectomy, splenectomy, nephrectomy, colectomy, and gastrectomy) in female Wistar albino rats 41 . Of these procedures, rats that underwent a colectomy exhibited CRP levels elevated over the control, gastrectomy, hepatectomy, and nephrectomy at 48 h postsurgery, possibly due to the release of bacteria during colon resection 41 . Because of limited study of CRP, other markers such as α2M and TNF‐α are recommended over CRP for longitudinal tracking of physically‐induced inflammation.…”

Section: Models Of Injurymentioning

confidence: 99%

“…Additionally, IL‐6 has been studied in response to various abdominal surgeries (hepatectomy, splenectomy, nephrectomy, colectomy, and gastrectomy) in female Wistar albino rats 41 . When averaging IL‐6 levels across all surgery procedures, levels were highest at 6 h postsurgery (compared to 24 and 48 h) 41 . The factors surgery and time displayed overall effects, and there was an interaction between the two factors 41 .…”

Section: Models Of Injurymentioning

confidence: 99%

“…When averaging IL‐6 levels across all surgery procedures, levels were highest at 6 h postsurgery (compared to 24 and 48 h) 41 . The factors surgery and time displayed overall effects, and there was an interaction between the two factors 41 . At 6 h, rats with gastrectomy had the highest IL‐6 levels (elevated over the splenectomy, colectomy, laparotomy (sham), and uninjured control), but at 24 h, rats with hepatectomy had the highest IL‐6 levels (elevated over the splenectomy, colectomy, sham, and control) 41 .…”

Section: Models Of Injurymentioning

confidence: 99%

“…At 6 h, rats with gastrectomy had the highest IL‐6 levels (elevated over the splenectomy, colectomy, laparotomy (sham), and uninjured control), but at 24 h, rats with hepatectomy had the highest IL‐6 levels (elevated over the splenectomy, colectomy, sham, and control) 41 . At 48 h, only gastrectomy and hepatectomy had levels higher than the sham group 41 . Further exploration of IL‐6 in response to surgically‐induced inflammation may help to determine its sensitivity to differentiating inflammatory stimuli.…”

Section: Models Of Injurymentioning

confidence: 99%

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Review of immunological plasma markers for longitudinal analysis of inflammation and infection in rat models

Gautreaux

Tucker

Person

et al. 2022

Journal Orthopaedic Research

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Disease or trauma of orthopedic tissues, including osteomyelitis, osteoporosis, arthritis, and fracture, results in a complex immune response, leading to a change in the concentration and milieu of immunological cells and proteins in the blood. While C-reactive protein levels and white blood cell counts are used to track inflammation and infection clinically, controlled longitudinal studies of disease/injury progression are limited.Thus, the use of clinically-relevant animal models can enable a more in-depth understanding of disease/injury progression and treatment efficacy. Though longitudinal tracking of immunological markers has been performed in rat models of various inflammatory and infectious diseases, currently there is no consensus on which markers are sensitive and reliable for tracking levels of inflammation and/or infection. Here, we discuss the blood markers that are most consistent with other outcome measures of the immune response in the rat, by reviewing their utility for longitudinal tracking of infection and/or inflammation in the following types of models: localized inflammation/arthritis, injury, infection, and injury + infection. While cytokines and acute phase proteins such as haptoglobin, fibrinogen, and α 2 -macroglobulin demonstrate utility for tracking immunological response in many inflammation and infection models, there is likely not a singular superior marker for all rat models. Instead, longitudinal characterization of these models may benefit from evaluation of a collection of cytokines and/or acute phase proteins.Identification of immunological plasma markers indicative of the progression of a pathology will allow for the refinement of animal models for understanding, diagnosing, and treating inflammatory and infectious diseases of orthopedic tissues.

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Microbiota transfer following liver surgery involves microbial extracellular vesicle migration that affects liver immunity

Cohen

Meyerovich

Blank

et al. 2023

Hepatology Communications

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Background: Short-term perioperative administration of probiotics was shown to alleviate postoperative complications and promote liver recovery among patients undergoing resection for liver malignancy. The mechanisms by which probiotic bacteria effectively influence the gut microbiome composition during the perioperative time are controversial. Here, we aim to elucidate the short-term direct biological effect of probiotic microbiota–derived vesicles on host liver cells during the perioperative period. Methods: Probiotic-derived vesicles (pbMVs) were administered postoperatively. pbMVs were isolated and characterized from probiotics, mainly from the bacteria genus Lactobacillus, Bifidobacterium, and Lactococcus. Mice underwent bile duct ligation, sham laparotomy (SHAM), or 70% partial hepatectomy (70%PH). pbMVs were tracked in vivo, and intrahepatic cellular and molecular aspects were analyzed by flow cytometry and qRT-PCR techniques. Liver sinusoidal endothelial cells (LSECs) analysis for Vascular Cell Adhesion Molecule-1(VCAM-1) expression following pbMV stimulation of cultured liver non-parenchymal cells which had been activated by LPS. Results: The administered pbMV rapidly translocated to the liver after surgery. pbMV administrations following surgeries enhanced neutrophil clearance; there was a dramatic decline in the liver neutrophil-to-lymphocyte ratio Ly6G+/CD3+ and an increase in IL6 levels. pbMVs reduced intrahepatic VCAM1 and ICAM2 expression compared with control following SHAM and decrease in IL10 levels following 70%PH. The administration of pbMV improved liver regeneration 72 hours following surgical liver resection with a significant decrease in IL17 expression. pbMVs modulated VCAM-1 on liver sinusoidal endothelial cells in liver cell culture. Conclusions: Our study findings provide mechanistic insights into the liver-gut axis following surgery and illustrate how probiotic vesicles can reduce adhesion molecule expression and affect immune cell invasion and liver immunity, resulting in improved liver recovery following hepatic surgery.

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Inflammatory response markers in rats undergoing abdominal surgical procedures

Cited by 6 publications

References 43 publications

Comparison of Sleeve Lobectomy for Lung Cancer Using Mini-Thoracotomy and an Optimized Robot-Assisted Technique

Comparison of Sleeve Lobectomy for Lung Cancer Using Mini-Thoracotomy and an Optimized Robot-Assisted Technique

Review of immunological plasma markers for longitudinal analysis of inflammation and infection in rat models

Microbiota transfer following liver surgery involves microbial extracellular vesicle migration that affects liver immunity

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