Inflammatory markers in chronic kidney disease and end stage renal disease patients

Zakaria, Mahmoud M.; Derbala, Safaa A.; Salem, Amany A.; El‐Agroudy, Amgad E.; El-Tantawy, Fatma M.

doi:10.1007/s11033-021-06684-4

Cited by 1 publication

(1 citation statement)

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“…These molecules lead to neutrophils’ and macrophages’ recruitment to injury area ( 12 , 38 – 41 ). Uncontrolled inflammation mediated by Fas/FasL plays an immunopathological dysregulated immune response and increased neutrophil counts in various tissue injuries, including respiratory and neurological disorders ( 12 , 42 – 46 ). An important adverse effect of hyperinflammation and cell apoptosis in COVID-19 patients is lymphopenia, that is affected by Fas/FasL ( 47 ).…”

Section: Discussionmentioning

confidence: 99%

Serum soluble Fas ligand is a severity and mortality prognostic marker for COVID-19 patients

et al. 2022

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Finding cytokine storm initiator factors associated with uncontrolled inflammatory immune response is necessary in COVID-19 patients. The aim was the identification of Fas/Fas Ligand (FasL) role in lung involvement and mortality of COVID-19 patients. In this case-control study, mild (outpatient), moderate (hospitalized), and severe (ICU) COVID-19 patients and healthy subjects were investigated. RNA isolated from PBMCs for cDNA synthesis and expression of mFas/mFasL mRNA was evaluated by RT-PCR. Serum sFas/sFasL protein by ELISA and severity of lung involvement by CT-scan were evaluated. Also, we docked Fas and FasL via Bioinformatics software (in silico) to predict the best-fit Fas/FasL complex and performed molecular dynamics simulation (MDS) in hyponatremia and fever (COVID-19 patients), and healthy conditions. mFasL expression was increased in moderate and severe COVID-19 patients compared to the control group. Moreover, mFas expression showed an inverse correlation with myalgia symptom in COVID-19 patients. Elevation of sFasL protein in serum was associated with reduced lung injury and mortality. Bioinformatics analysis confirmed that blood profile alterations of COVID-19 patients, such as fever and hyponatremia could affect Fas/FasL complex interactions. Our translational findings showed that decreased sFasL is associated with lung involvement; severity and mortality in COVID-19 patients. We think that sFasL is a mediator of neutrophilia and lymphopenia in COVID-19. However, additional investigation is suggested. This is the first report describing that the serum sFasL protein is a severity and mortality prognostic marker for the clinical management of COVID-19 patients.

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