Inflammatory marker analysis in psoriatic skin under topical phosphodiesterase 4 inhibitor treatment

Roesner, Lennart M.; Kienlin, Petra; Begemann, Gabriele; Dittrich–Breiholz, Oliver; Werfel, Thomas

doi:10.1016/j.jaci.2017.04.026

Cited by 4 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To underline the clinical importance of these marker genes, we utilized bulk transcriptomics data from an independent set of samples comparing PS skin lesions before and after treatment: Previously, we reported pathological upregulation of a set of genes in PS and demonstrated successful treatment with the phosphodiesterase‐inhibitor roflumilast led to downregulation of these genes, underlining their pathogenic importance 21 . Among our markers identified in PS severity‐associated Th17/Tc17 clusters, several genes, including CXCL13 , GZMB , IL26 , and IL17F (Figure 3G), were also upregulated in the bulk expression data from PS patients and subsequently recovered following treatment (Table S5).…”

Section: Resultsmentioning

confidence: 99%

“…Bulk TCR‐seq datasets from skin of 11 AD and 11 PS samples were obtained from Roesner et al 19 Five additional healthy controls (HC), analyzed by the same technique, were obtained from Harden et al 20 These bulk TCR‐seq datasets were analyzed together with scTCR‐seq data to evaluate the shared clonally propagated TCR motifs. Bulk RNA‐seq with or without successful treatment with Roflumilast, a phosphodiesterase inhibitor, were obtained from Roesner et al 21 to evaluate the transcriptional responses of disease‐specific T‐cell signature genes from therapeutic treatments (for detailed validation calculations, please see supplementary materials and methods).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Single‐cell profiles reveal distinctive immune response in atopic dermatitis in contrast to psoriasis

Zhang

Roesner

Traidl

et al. 2022

Allergy

Self Cite

View full text Add to dashboard Cite

Background Understanding the complex orchestrated inflammation in atopic dermatitis (AD), one of the most common chronic inflammatory diseases worldwide, is essential for therapeutic approaches. However, a comparative analysis on the single‐cell level of the inflammation signatures correlated with the severity is missing so far. Methods We applied single‐cell RNA and T‐cell receptor (TCR) sequencing on immune cells enriched from skin biopsies and matched blood samples of AD in comparison with psoriasis (PS) patients. Results Clonally propagated skin‐derived T cells showed disease‐specific TCR motifs shared between patients which was more pronounced in PS compared to AD. The disease‐specific T‐cell clusters were mostly of a Th2/Th22 sub‐population in AD and Th17/Tc17 in PS, and their numbers were associated with severity scores in both diseases. Herein, we provide for the first time a list that associates cell type‐specific gene expression with the severity of the two most common chronic inflammatory skin diseases. Investigating the cell signatures in the patients´ PBMCs and skin stromal cells, a systemic involvement of type‐3 inflammation was clearly detectable in PS circulating cells, while in AD inflammatory signatures were most pronounced in fibroblasts, pericytes, and keratinocytes. Compositional and functional analyses of myeloid cells revealed the activation of antiviral responses in macrophages in association with disease severity in both diseases. Conclusion Different disease‐driving cell types and subtypes which contribute to the hallmarks of type‐2 and type‐3 inflammatory signatures and are associated with disease activities could be identified by single‐cell RNA‐seq and TCR‐seq in AD and PS.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Single‐cell profiles reveal distinctive immune response in atopic dermatitis in contrast to psoriasis

Zhang

Roesner

Traidl

et al. 2022

Allergy

Self Cite

View full text Add to dashboard Cite

show abstract

“…functions such as inflammasome formation, signaling transduction, transcription activation, and autophagy (Kim et al, 2016). The increase in beta-defensin 2 protein is considered pro-inflammatory and innate immune response, which has marked antimicrobial properties (Roesner et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profile in Immortalized Human Periodontal Ligament Fibroblasts Through hTERT Ectopic Expression: Transcriptome and Bioinformatic Analysis

Nogueira

Vasconcelos

Mitre

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

Human periodontal ligament fibroblast (hPLF) cells play an important role in maintaining oral cavity homeostasis with special function in tissue regeneration and maintenance of dental alveoli. Although their primary cell cultures are considered a good experimental model with no genetic changes, the finite life span may limit some experimental designs. The immortalization process increases cell life span but may cause genetic changes and chromosomal instability, resulting in direct effects on physiological cell responses. In this way, we aimed to investigate the global gene expression of hPLFs after the immortalization process by the ectopic expression of the catalytic subunit of the enzyme telomerase reverse transcriptase (hTERT) through transcriptome analysis. The embryonic origin of the primary culture of hPLF cells and immortalized hPLF-hTERT was also tested by vimentin staining, hTERT synthesis evaluated by indirect immunocytochemistry, analysis of cell proliferation, and morphology. The results indicated that hPLFs and hPLF-hTERT were positive for vimentin. On the 20th cell passage, hPLFs were in senescence, while hPLF-hTERT maintained their proliferation and morphology characteristics. At the same passage, hPLF-hTERT presented a significant increase in hTERT synthesis, but transcriptome did not reveal overexpression of the hTERT gene. Fifty-eight genes had their expression altered (11 upregulated and 47 downregulated) with the absence of changes in the key genes related to these cell types and in the main cancer-associated genes. In addition, the increase in hTERT protein expression without the overexpression of its gene indicates posttranscriptional level regulation. Successful immortalization of hPLFs through the ectopic expression of hTERT encourages further studies to design experimental protocols to investigate clinical questions from a translational perspective.

show abstract

Exploration of novel candidate genes involved in epidermal keratinocyte differentiation and skin barrier repair in man

et al. 2021

View full text Add to dashboard Cite

Inflammatory marker analysis in psoriatic skin under topical phosphodiesterase 4 inhibitor treatment

Cited by 4 publications

References 18 publications

Single‐cell profiles reveal distinctive immune response in atopic dermatitis in contrast to psoriasis

Single‐cell profiles reveal distinctive immune response in atopic dermatitis in contrast to psoriasis

Gene Expression Profile in Immortalized Human Periodontal Ligament Fibroblasts Through hTERT Ectopic Expression: Transcriptome and Bioinformatic Analysis

Exploration of novel candidate genes involved in epidermal keratinocyte differentiation and skin barrier repair in man

Contact Info

Product

Resources

About