Inflammatory gene networks in term human decidual cells define a potential signature for cytokine-mediated parturition

Ibrahim, Sherrine; Ackerman, William E.; Summerfield, Taryn; Lockwood, Charles J.; Schatz, Frederick; Kniss, Douglas A.

doi:10.1016/j.ajog.2015.08.075

Cited by 30 publications

(32 citation statements)

References 80 publications

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“…Among highly up-regulated transcripts were pro-inflammatory cytokines (TNF, IL1A IL6), different chemokines and prostaglandin synthesizing enzymes. Pathway enrichment analysis provided cytokine-cytokine receptor interaction, apoptosis, Janus kinase signal transducer and STAT signaling [16]. This study supports the concept of up-regulation of genes involved in pro-inflammatory pathways at the time of delivery.…”

Section: Comparison Of Gene Expression In the Cord Blood Between Subgsupporting

confidence: 65%

“…An experimental study on fibroblast culture of term decidual cells obtained from uncomplicated term pregnancies after elective CS and before the onset of labor was performed by the group from The Ohio State University [16]. After fibroblast challenge with IL1 beta for 6 hours up-regulation 448 transcripts and down-regulation of 116 transcripts was observed.…”

Section: Comparison Of Gene Expression In the Cord Blood Between Subgmentioning

confidence: 99%

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Comparison of whole genome expression profile between preterm and full-term newborns

et al. 2017

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Objectives: Evaluate the time dependent expression of genes in preterm neonates and verify the influence of ontogenic maturation and the environmental factors on the gene expression after birth. Material and methods:The study was carried out on 20 full-term newborns and 62 preterm newborns (mean birth weight = 1002 [g] (SD: 247), mean gestational age = 27.2 weeks (SD: 1.9)). Blood samples were drawn from all the study participants at birth and at the 36th week postmenstrual age from the preterm group to assess whole genome expression in umbilical cord blood and in peripheral blood leukocytes, respectively. (SurePrint G3 Human Gene Expression v3, 8x60K Microarrays (Agilent)).Results: A substantial number of genes was found to be expressed differentially at the time of birth and at 36 PMA in comparison to the term babies with more genes being down-regulated than up-regulated. However, the fold change in the majority of cases was < 2.0. Extremely preterm and very preterm infants were characterized by significantly down-regulated cytokine and chemokine related pathways. The number of down-regulated genes decreased and number of up-regulated genes increased at 36 PMA vs. cord blood. There were no specific gene expression pathway profiles found within the groups of different gestational ages. Conclusions:Preterm delivery is associated with a different gene expression profile in comparison to term delivery. The gene expression profile changes with the maturity of a newborn measured by the gestational age.

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Section: Comparison Of Gene Expression In the Cord Blood Between Subgsupporting

confidence: 65%

Section: Comparison Of Gene Expression In the Cord Blood Between Subgmentioning

confidence: 99%

Comparison of whole genome expression profile between preterm and full-term newborns

et al. 2017

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“…Our data suggests that the increased decidual IL1B expression in TIL cases compared to TNL cases is consistent with these earlier findings and further highlights the key role of IL-1β in parturition. As functional evidence, in vitro experiments with human decidual cells revealed that IL-1β treatment alters the expression of genes and microRNAs that function in pro-inflammatory signaling, including the induction of numerous cytokines and chemokines [139], such as IL-8 [140], as well as prostaglandin production [141]. …”

Section: Discussionmentioning

confidence: 99%

Characteristic Changes in Decidual Gene Expression Signature in Spontaneous Term Parturition

Elazzamy

Balogh

Romero

et al. 2017

J Pathol Transl Med

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BackgroundThe decidua has been implicated in the “terminal pathway” of human term parturition, which is characterized by the activation of pro-inflammatory pathways in gestational tissues. However, the transcriptomic changes in the decidua leading to terminal pathway activation have not been systematically explored. This study aimed to compare the decidual expression of developmental signaling and inflammation-related genes before and after spontaneous term labor in order to reveal their involvement in this process. MethodsChorioamniotic membranes were obtained from normal pregnant women who delivered at term with spontaneous labor (TIL, n = 14) or without labor (TNL, n = 15). Decidual cells were isolated from snap-frozen chorioamniotic membranes with laser microdissection. The expression of 46 genes involved in decidual development, sex steroid and prostaglandin signaling, as well as pro- and anti-inflammatory pathways, was analyzed using high-throughput quantitative real-time polymerase chain reaction (qRT-PCR). Chorioamniotic membrane sections were immunostained and then semi-quantified for five proteins, and immunoassays for three chemokines were performed on maternal plasma samples. ResultsThe genes with the highest expression in the decidua at term gestation included insulin-like growth factor-binding protein 1 (IGFBP1), galectin-1 (LGALS1), and progestogen-associated endometrial protein (PAEP); the expression of estrogen receptor 1 (ESR1), homeobox A11 (HOXA11), interleukin 1β (IL1B), IL8, progesterone receptor membrane component 2 (PGRMC2), and prostaglandin E synthase (PTGES) was higher in TIL than in TNL cases; the expression of chemokine C-C motif ligand 2 (CCL2), CCL5, LGALS1, LGALS3, and PAEP was lower in TIL than in TNL cases; immunostaining confirmed qRT-PCR data for IL-8, CCL2, galectin-1, galectin-3, and PAEP; and no correlations between the decidual gene expression and the maternal plasma protein concentrations of CCL2, CCL5, and IL-8 were found. ConclusionsOur data suggests that with the initiation of parturition, the decidual expression of anti-inflammatory mediators decreases, while the expression of pro-inflammatory mediators and steroid receptors increases. This shift may affect downstream signaling pathways that can lead to parturition.

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“…The amniotic fluid concentration of HBD-1 was similar between women with and without spontaneous labor at term. Labor is considered a state of physiologic inflammation [151][152][153][154][155][156][157][158][159][160][161][162][163][164][165][166][167] as intra-amniotic infection is absent in most women who deliver at term. 117 Therefore, spontaneous labor at term is a sterile inflammatory process and may not need to be associated with an increase in the concentration of HBD-1.…”

Section: Amniotic Fluid Hbd-1 Concentrations Do Not Differ Between mentioning

confidence: 99%

Human β‐defensin‐1: A natural antimicrobial peptide present in amniotic fluid that is increased in spontaneous preterm labor with intra‐amniotic infection

Varrey

Romero

Panaitescu

et al. 2018

American J Rep Immunol

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Inflammatory gene networks in term human decidual cells define a potential signature for cytokine-mediated parturition

Cited by 30 publications

References 80 publications

Comparison of whole genome expression profile between preterm and full-term newborns

Comparison of whole genome expression profile between preterm and full-term newborns

Characteristic Changes in Decidual Gene Expression Signature in Spontaneous Term Parturition

Human β‐defensin‐1: A natural antimicrobial peptide present in amniotic fluid that is increased in spontaneous preterm labor with intra‐amniotic infection

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