Inflammatory Correlated Response in Two Lines of Rabbit Selected Divergently for Litter Size Environmental Variability

Beloumi, Dhekra; Blasco, A.; Muelas, R.; Santacreu, M. A.; Garcia, María de la Luz; Argente, M. J.

doi:10.3390/ani10091540

Cited by 13 publications

(24 citation statements)

References 46 publications

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“…LPS increased the expression of LDLr at the mRNA level and intracellular cholesterol ester content. Previously, studies have shown that intracellular cholesterol accumulation could obstruct mitochondrial function [30] and increase sensitivity to inflammation [31], thus affecting negative feedback regulation of LDLr [32,33]. Ma et al also reproted that inflammation increased lipid accumulation in VSMCs by disrupting LDLr feedback regulation [34], which further supports our present findings.…”

Section: Furthermore Macrophage-derived Foam Cells Have Attracted Extensive Scholars' Attention Whilesupporting

confidence: 91%

Golgi α-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress

Zha

2021

Folia Histochem Cytobiol.

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Introduction. Vascular smooth muscle cells (VSMCs)-based foam cell formation is a crucial factor in the atherosclerosis process. We aimed to explore the mechanism of Golgi α-mannosidase II (GMII) effects on the VSMCs-based foam cell formation. Material and methods. VSMCs were exposed to different concentrations of low-density lipoproteins (LDLs), lipopolysaccharide (LPS), and/or GMII inhibitor (swainsonine). The qRT-PCR and western blot were used for expression analysis. Oil Red O staining was used to verify changes of lipid droplets in VSMCs. The translocation of the SCAP from the endoplasmic reticulum (ER) to Golgi was detected by immunofluorescence (IF) staining. Results. LPS disrupted the LDLs-mediated regulation of LDL receptor (LDLr) and increased intracellular cholesterol ester, which was inversely inhibited by swainsonine. The activity of α-mannosidase II and GMII expression were decreased by LDLs but increased by the addition of LPS. Conversely, LPS-induced enhancement was reversed by swainsonine. Additionally, swainsonine reversed the LPS-induced increase of intracellular lipid droplets in the presence of LDLs. Expression analysis demonstrated that LDLr, SCAP, and SREBP2 were up-regulated by LPS, but reversed by swainsonine in LDLs-treated cells. IF staining revealed that swainsonine inhibited the translocation of SCAP to Golgi under inflammatory stress. Conclusions. Collectively, swainsonine restrained LDLr expression to suppress the formation of VSMCs-based foam cells by reducing SREBP2 and SCAP under inflammatory stress conditions, suggesting that GMII contributes to the formation of VSMCs-based foam cells under inflammatory stress.

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Section: Furthermore Macrophage-derived Foam Cells Have Attracted Extensive Scholars' Attention Whilesupporting

confidence: 91%

Golgi α-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress

Zha

2021

Folia Histochem Cytobiol.

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“…There were also differences in sensitivity to stress and diseases, which lowered the culling rate in the low line [ 11 ]. In this regard, the more homogeneous line coped better with environmental stressors such as infections and acute stress than the heterogeneous line which showed higher resilience [ 11 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Correlated Response to Selection for Litter Size Residual Variability in Rabbits’ Body Condition

Agea

Garcia

Blasco

et al. 2020

Animals

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A divergent selection experiment for residual variance of litter size at birth was carried out in rabbits during twelve generations. Residual variance of litter size was estimated as the within-doe variance of litter size after pre-correction for year and season as well as parity and lactation status effects. The aim of this work was to study the correlated response to selection for litter size residual variability in body condition from mating to weaning. Body condition is related directly to an animal’s fat deposits. Perirenal fat is the main fat deposit in rabbits. Individual body weight (IBW) and perirenal fat thickness (PFT) were used to measure body condition at second mating, delivery, 10 days after delivery, and weaning. Litter size of the first three parities was analyzed. Both lines decreased body condition between mating to delivery; however, the decrease in body condition at delivery was lower in the low line, despite this line having higher litter size at birth (+0.54 kits, p = 0.93). The increment of body condition between delivery and early lactation was slightly higher in the low line. On the other hand, body condition affected success of females’ receptivity and fertility at the third mating, e.g., receptive females showed a higher IBW and PFT than unreceptive ones (+129 g and +0.28 mm, respectively), and fertile females had a higher IBW and PFT than unfertile ones (+82 g and +0.28 mm, respectively). In conclusion, the does selected for reducing litter size variability showed a better deal with situations of high-energy demand, such as delivery and lactation, than those selected for increasing litter size variability, which would agree with the better health and welfare condition in the low line.

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“…Hematological parameters provide valuable information on the health status of the animal. In the present study, the hematological profiles were within the range for healthy rabbits both at the beginning and end of the experiment and for both diets [ 18 , 23 ]. Levels of albumin, alkaline phosphatase, alanine aminotransferase, total bilirubin, total protein, globulin, glucose, creatinine, uric nitrogen and amylase are within the wide range of values reported in rabbits [ 18 , 24 , 25 ].…”

Section: Discussionmentioning

confidence: 77%

“…Following the blood sampling procedure described in [ 18 ], blood samples were collected into a tube with tripotassium ethylenediaminetetraacetic acid (K3-EDTA) at weeks 0 and 15. Hematological parameters such as white blood leukocyte count (WBC, 103/μL) and percentage of lymphocytes, neutrophils, monocytes, basophils and eosinophils were determined with the Abacus Junior Vet hematology analyzer (Diatron, Austria).…”

Section: Methodsmentioning

confidence: 99%

Effect of Postbiotic Based on Lactic Acid Bacteria on Semen Quality and Health of Male Rabbits

Cano¹,

Argente

García

2021

Animals

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The aim of this study was to evaluate the effect of lactic acid bacteria-based postbiotic supplementation on semen characteristics and hematological and biochemical profiles in rabbits. A total of 28 males were randomly allocated into two groups. Males received a Control diet and Enriched diet supplemented with postbiotic for 15 weeks (4 weeks of adaptation period and 11 weeks of experimental period). Body weight, feed intake and semen characteristics were recorded weekly. Hematological profile was recorded at the beginning and end of the experiment and biochemical profile at 0, 5, 10 and 15 weeks. Bayesian methodology was used for the statistical analysis. Feed intake was higher in Control diet (125.2 g) than in the Enriched diet (118.6 g, p = 1.00). The percentages of abnormal spermatozoa were higher in Control diet than in Enriched diet (30% and 22%; p = 0.93) and the acrosome integrity percentage was lower (97% and 96%; p = 0.87). The hematological profile was within the range for healthy rabbits. The plasmatic level of alanine aminotransferase was higher in Control diet than Enriched diet at 5 and 10 weeks (p = 0.93 and p = 0.94, respectively) and alkaline phosphatase was similar in Control diet throughout the experiment, but decreased in Enriched diet (p = 0.97). No difference was found in kidney parameters (uric nitrogen and creatinine). Enriched diet showed higher total protein and globulin than Control diet (p = 0.99). Phosphorus was lower (p = 0.92) in Control diet than in Enriched diet. In conclusion, the addition of the postbiotic based on lactic acid bacteria seems to improve the quality of the semen and the liver profile in rabbits.

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Inflammatory Correlated Response in Two Lines of Rabbit Selected Divergently for Litter Size Environmental Variability

Cited by 13 publications

References 46 publications

Golgi α-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress

Golgi α-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress

Correlated Response to Selection for Litter Size Residual Variability in Rabbits’ Body Condition

Effect of Postbiotic Based on Lactic Acid Bacteria on Semen Quality and Health of Male Rabbits

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