Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis

Facchin, B.; Reis, Gustavo Oliveira dos; Vieira, Guilherme Nicácio; Mohr, Eduarda Talita Bramorski; Rosa, Júlia Salvan da; Kretzer, Iara Fabrícia; Demarchi, Izabel Galhardo; Dalmarco, Eduardo Monguilhott

doi:10.1007/s00011-022-01584-0

Cited by 64 publications

(43 citation statements)

References 51 publications

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“…Furthermore, we used RAW 264.7 cells to evaluate the sensing ability of BDP3 toward endogenous NO (Figure D). RAW 264.7 is an excellent model to investigate the in vitro behaviors of macrophages . In response to inflammatory stimuli including IFN-γ and LPS, RAW 264.7 cells could act as proinflammatory M1 macrophages to produce inducible nitric oxide synthase (iNOS), which catalyzes the production of NO from l -arginine .…”

Section: Resultsmentioning

confidence: 99%

“…33 In response to inflammatory stimuli including IFN-γ and LPS, RAW 264.7 cells could act as proinflammatory M1 macrophages to produce inducible nitric oxide synthase (iNOS), which catalyzes the production of NO from L-arginine. 33 In contrast, IL-4, which is an M2 macrophage promoter, can inhibit RAW 264.7 cells to produce proinflammatory cytokines. As illustrated in Figure 2E, compared to control groups, IL-4-treated groups showed negligible fluorescence, while IFN-γ/LPS-treated groups exhibited bright red fluorescence, proving that BDP3 could recognize endogenous NO.…”

Section: •−mentioning

confidence: 99%

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BODIPY-Based NO Probe for Macrophage-Targeted Immunotherapy Response Monitoring

Chen

Wang

et al. 2023

Anal. Chem.

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Precise and rapid detection of immune responses is critical for timely therapeutic regimen adjustment. Immunomodulation of tumor-associated macrophages (TAMs) from a protumorigenic phenotype (M2) to an antitumorigenic phenotype (M1) is crucial in macrophage-targeted immunotherapy. Herein, we developed a boron dipyrromethene (BODIPY)-based fluorescence probe BDP3 to detect the immune responses after immunotherapy by monitoring the nitric oxide (NO) released by M1 TAMs. With an aromatic primary monoamine structure and a p-methoxyanilin electron donor in the meso-position, BDP3 not only specifically activates stable and sensitive fluorescence by NO via a photoinduced electron transfer (PET) process but also achieves a long emission wavelength for efficient in vitro and in vivo imaging. Such NO-induced fluorescence signals of BDP3 are validated to correlate well with the phenotypes of TAMs detected in macrophage cell lines and tumor tissues. The distinct sensing effects toward two types of clinically used immunotherapeutic drugs further confirm the ability of BDP3 for specific monitoring of the M1/M2 switch in response to the macrophage-targeted immunotherapy. By virtue of good biocompatibility and appropriate tumor retention time, BDP3 could be a potential fluorescent probe for noninvasive evaluation of the immunotherapeutic efficacy of macrophage-targeted immunotherapy in living animals.

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Section: Resultsmentioning

confidence: 99%

Section: •−mentioning

confidence: 99%

BODIPY-Based NO Probe for Macrophage-Targeted Immunotherapy Response Monitoring

Chen

Wang

et al. 2023

Anal. Chem.

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“…NF-κB pathway has been defined as a key participant in LPS infections [ 18 , 19 ]. Meanwhile, hypoxia-inducible factor-1α (HIF-1α) is considered one of endogenous ROS's core cellular targets, which further regulates the hypoxia signaling in the inflammatory response [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis and identification reveal the anti-inflammatory mechanism of clofazimine on lipopolysaccharide-induced acute lung injury in mice

Yang

Gao

et al. 2022

Inflamm. Res.

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Background and objective Acute lung injury (ALI)/ acute respiratory distress syndrome (ARDS) was increasingly recognized as one of the most severe acute hyperimmune response of coronavirus disease 2019 (COVID-19). Clofazimine (CFZ) has attracted attention due to its anti-inflammatory property in immune diseases as well as infectious diseases. However, the role and potential molecular mechanism of CFZ in anti-inflammatory responses remain unclear. Methods We analyze the protein expression profiles of CFZ and LPS from Raw264.7 macrophages using quantitative proteomics. Next, the protective effect of CFZ on LPS-induced inflammatory model is assessed, and its underlying mechanism is validated by molecular biology analysis. Results LC–MS/MS-based shotgun proteomics analysis identified 4746 (LPS) and 4766 (CFZ) proteins with quantitative information. The key proteins and their critical signal transduction pathways including TLR4/NF-κB/HIF-1α signaling was highlighted, which was involved in multiple inflammatory processes. A further analysis of molecular biology revealed that CFZ could significantly inhibit the proliferation of Raw264.7 macrophages, decrease the levels of TNF-α and IL-1β, alleviate lung histological changes and pulmonary edema, improve the survival rate, and down-regulate TLR4/NF-κB/HIF-1α signaling in LPS model. Conclusion This study can provide significant insight into the proteomics-guided pharmacological mechanism study of CFZ and suggest potential therapeutic strategies for infectious disease. Supplementary Information The online version contains supplementary material available at 10.1007/s00011-022-01623-w.

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“…On the other hand, inflammatory macrophages secrete inflammatory cytokines including IL-1, TNF-α, and IL-6, and exacerbate the inflammatory reaction of the artery wall [ 21 ]. Raw264.7, a mouse macrophage cell-line, characterized by significant phagocytosis and pseudopodial movement, was widely used in the research of the phenotype and the function of the macrophage [ 22 ]. The results of cellular immunofluorescence revealed that Raw264.7 was highly positive for the macrophage marker CD68 ( Figure S3 ).…”

Section: Resultsmentioning

confidence: 99%

Self-Assembled CuCo2S4 Nanoparticles for Efficient Chemo-Photothermal Therapy of Arterial Inflammation

Wang

Dong

et al. 2022

Molecules

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Cardiovascular disease caused by atherosclerosis (AS) seriously affects human health. Photothermal therapy (PTT) brings hope to the diagnosis and treatment of AS, with the development of nanotechnology. To improve treatment efficiency, self-assembled CuCo2S4 nanocrystals (NCs) were developed as a drug-delivery nanocarrier, triggered by near-infrared (NIR) light for efficient chemophotothermal therapy of arterial inflammation. The as-prepared self-assembled CuCo2S4 NCs exhibited excellent biocompatibility and a very high chloroquine (CL)-loading content. In addition, the self-assembled CuCo2S4 NCs/CL nanocomposites showed good photothermal performance, due to strong absorption in the NIR region, and the release of CL from the NCs/CL nanocomposites was driven by NIR light. When illuminated by NIR light, both PTT from the NCs and chemotherapy from the CL were simultaneously triggered, resulting in killing macrophages with a synergistic effect. Moreover, chemo-photothermal therapy with CuCo2S4 NCs/CL nanocomposites showed an effective therapeutic effect for arterial inflammation, in vivo. Our work demonstrated that chemo-photothermal therapy could be a promising strategy for the treatment of arterial inflammation against atherosclerosis.

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Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis

Cited by 64 publications

References 51 publications

BODIPY-Based NO Probe for Macrophage-Targeted Immunotherapy Response Monitoring

BODIPY-Based NO Probe for Macrophage-Targeted Immunotherapy Response Monitoring

Proteomic analysis and identification reveal the anti-inflammatory mechanism of clofazimine on lipopolysaccharide-induced acute lung injury in mice

Self-Assembled CuCo2S4 Nanoparticles for Efficient Chemo-Photothermal Therapy of Arterial Inflammation

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