Inflammatory biomarkers in osteoarthritis

Daghestani, Hikmat N.; Kraus, Virginia B.

doi:10.1016/j.joca.2015.02.009

Cited by 137 publications

(106 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The increase in lipid and protein levels may be attributed to the accumulation of lipids and total fatty acids (such as hypercholesterolaemia), and increased protein expression levels, which have been previously reported in OA . Other studies have shown that inflammatory mediators are increased in OA in comparison to healthy subjects, which can be reflected outside the joint tissues in blood plasma and peripheral blood leucocytes . Similar to a number of preceding studies demonstrating increased levels of collagen in OA subjects after analysing cartilage or blood samples, our results also showed a statistically significant increase of the collagen band (1034 cm −1 ) in OA (Fig ) .…”

Section: Discussionsupporting

confidence: 90%

Attenuated total reflection Fourier‐transform infrared (ATR‐FTIR) spectroscopy to diagnose osteoarthritis in equine serum

Paraskevaidi

Hook

Morais

et al. 2019

Equine Veterinary Journal

View full text Add to dashboard Cite

Summary Background Reliable and validated biomarkers for osteoarthritis (OA) are currently lacking. Objectives To develop an accurate and minimally invasive method to assess OA‐affected horses and provide potential spectral markers indicative of disease. Study design Observational, cross‐sectional study. Methods Our cohort consisted of 15 horses with OA and 48 without clinical signs of the disease, which were used as controls. Attenuated total reflection Fourier‐transform infrared (ATR‐FTIR) spectroscopy was used to investigate serum samples (50 μL) collected from these horses. Spectral processing and multivariate analysis revealed differences and similarities, allowing for detection of spectral biomarkers that discriminated between the two cohorts. A supervised classification algorithm, namely principal component analysis coupled with quadratic discriminant analysis (PCA‐QDA), was applied to evaluate the diagnostic accuracy. Results Segregation between the two different cohorts, OA‐affected and controls, was achieved with 100% sensitivity and specificity. The six most discriminatory peaks were attributed to proteins and lipids. Four of the spectral peaks were elevated in OA horses, which could be potentially due to an increase in lipids, protein expression levels and collagen, all of which have been previously reported in OA. Two peaks were found decreased and were tentatively assigned to the reduction of proteoglycan content that is observed during OA. Main limitations The control group had a wide range of ages and breeds. Presymptomatic OA cases were not included. Therefore, it remains unknown whether this test could also be used as an early diagnostic tool. Conclusions This spectrochemical approach could provide an accurate and cost‐effective blood test, facilitating point‐of‐care diagnosis of equine OA.

show abstract

Section: Discussionsupporting

confidence: 90%

Attenuated total reflection Fourier‐transform infrared (ATR‐FTIR) spectroscopy to diagnose osteoarthritis in equine serum

Paraskevaidi

Hook

Morais

et al. 2019

Equine Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…In addition to epigenetic changes, it is also clear that chronic and low-grade inflammation is involved in the progression of OA (53–56) that leads to catabolic responses in chondrocytes via upregulation of factors such as nuclear factor-kappa B (NF-κB) (57), MMPs, and markers of chondrocyte hypertrophy (e.g., Col10a1 , Mmp13 , Runx2 , Alp ). Recent investigations from human patients as well as animal models suggest that the entire synovial joint, including articular cartilage, subchondral bone, synovial tissue, ligament, and meniscus, contribute to the inflammation network.…”

Section: Epigenetic and Inflammatory Changes In Oamentioning

confidence: 99%

Inflammation and epigenetic regulation in osteoarthritis

Shen

Abu‐Amer

O’Keefe

et al. 2016

Connective Tissue Research

188

140

View full text Add to dashboard Cite

Osteoarthritis (OA) was once defined as a non-inflammatory arthropathy, but it is now well-recognized that there is a major inflammatory component to this disease. In addition to synovial cells, articular chondrocytes and other cells of diarthrodial joints are also known to express inflammatory mediators. It has been proposed that targeting inflammation pathways could be a promising strategy to treat OA. There have been many reports of cross-talk between inflammation and epigenetic factors in cartilage. Specifically, inflammatory mediators have been shown to regulate levels of enzymes that catalyze changes in DNA methylation and histone structure, as well as alter levels of non-coding RNAs. In addition, expression levels of a number of these epigenetic factors have been shown to be altered in OA, thereby suggesting potential interplay between inflammation and epigenetics in this disease. This review provides information on inflammatory pathways in arthritis and summarizes published research on how epigenetic regulators are affected by inflammation in chondrocytes. Furthermore, we discuss data showing how altered expression of some of these epigenetic factors can induce either catabolic or anti-catabolic effects in response to inflammatory signals. A better understanding of how inflammation affects epigenetic factors in OA may provide us with novel therapeutic strategies to treat this condition.

show abstract

“…This in future could be a promising candidate to assess for association with OA Although previous studies have identified associations of pro-inflammatory cytokines with cartilage destruction, subchondral bone remodeling, synovial inflammation (7,11), and OA severity and progression (43)(44)(45), only a limited number of studies have reported correlations of sf IL-6, IL-8 and TNFα to levels of pain. As shown in animal studies, locally produced cytokines and chemokines can contribute to the sensitization of joint nociceptors (12), thereby identifying pro-inflammatory factors as etiologies of pain.…”

Section: Accepted Manuscriptmentioning

confidence: 99%