Inflammatory and regulatory CCL and CXCL chemokine and cytokine cellular responses in patients with patent<i>Mansonella perstans</i>filariasis

Wangala, Bawoubadi; Gantin, Richard G.; Voßberg, Patrick; Vovor, A.; Poutouli, Wiyao; Komlan, Kossi; Banla, M.; Köhler, Carsten; Soboslay, Peter T.

doi:10.1111/cei.13251

Cited by 8 publications

(5 citation statements)

References 45 publications

(85 reference statements)

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“…Analysis by one-way ANOVA test indicated significant differences among the different groups (*P < 0.05, **P < 0.01, ***P < 0.001 vs the control group. + P < 0.05, ++ P < 0.01, +++ P < 0.001 vs the CsTP+HBV group) ◂ or anti-inflammatory activities [38][39][40]. We previously revealed that CsTPs could promote the production of Th2 cytokines, including IL-13 and IL-4, both in vitro and in vivo, and ultimately resulted in the development of liver fibrosis [16].…”

Section: Discussionmentioning

confidence: 97%

Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models

et al. 2022

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Background In China, people infected with hepatitis B virus (HBV) are commonly found in areas with a high prevalence of Clonorchis sinensis, a trematode worm. Published studies have reported that the progression of hepatitis B is affected by coinfection C. sinensis. Methods Clinical data from a total of 72 patients with C. sinensis and HBV (as sole infection or with coinfections) and 29 healthy individuals were analysed. We also incubated the hepatic stellate cell line LX-2 with total proteins from C. sinensis adult worms (CsTPs) and HBV-positive sera. In addition, the human hepatoblastoma cell line HepG2.2.15 was treated with the antiviral drug entecavir (ETV), CsTPs and the anti-C. sinensis drug praziquantel (PZQ). Results Our clinical data indicated that the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB) and hyaluronic acid (HA) were significantly higher in patients with coinfection than in those infected with HBV only. In cell models, compared with the model in which LX-2 cells were incubated with HBV-positive sera (HBV group), transcripts of alpha-smooth muscle actin and types I and III collagen were significantly elevated in the models of LX-2 cells treated with CsTPs and HBV-positive sera (CsTP+HBV group), while the messenger RNA levels of tumour necrosis factor-α, interleukin (IL)-1β and IL-6 in the CsTP+HBV group were clearly lower. The HBV surface antigen and hepatitis B e-antigen levels were higher in the HepG2.2.15 cells treated with ETV and CsTPs than in those in the ETV group and in the cells administered a mixture of ETV, CsTPs and PZQ. Conclusions These results confirmed that C. sinensis and HBV coinfection could aggravate the progression of liver fibrosis. CsTPs might promote chronic inflammation of the liver in individuals with HBV infection, resulting in the development of hepatic fibrosis. Graphic abstract

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Section: Discussionmentioning

confidence: 97%

Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models

et al. 2022

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“…Although millions of humans are infected and suffer from severe pathology, knowledge about parasite evasion and survival tactics are not fully understood. Over the last decades, several studies showed that filariae modulate immunity in humans through the induction of Th2 and regulatory T and B cell populations accompanied with the suppression of pro-inflammatory and Th1 immune responses to guarantee long-term survival and reproduction of the parasite (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)63). Nevertheless, it is impossible to investigate the complexity of the filarial-driven modulation and survival tactics solely in humans due to limited access to parasitic life stages, unknown infection time point, possible re-and co-infections and most importantly ethical concerns.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, several studies have characterized host immunity during filarial infections and revealed that filarial infections dampen and modulate host immune responses via the induction of regulatory B and T cell subsets, Th2 cell populations and distinct patterns of cytokines, chemokines and immunoglobulins accompanied with the suppression of Th1 and pro-inflammatory cell subsets (10)(11)(12)(13)(14)(15)(16)(17)(18). This immunomodulation leads in most infected people to an asymptomatic clinical picture which is essential for long-term survival of the parasites (19,20), but is also suggested to influence disease outcome of concomitant infections as well as vaccination efficacy (21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses

et al. 2021

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Despite long-term mass drug administration programmes, approximately 220 million people are still infected with filariae in endemic regions. Several research studies have characterized host immune responses but a major obstacle for research on human filariae has been the inability to obtain adult worms which in turn has hindered analysis on infection kinetics and immune signalling. Although the Litomosoides sigmodontis filarial mouse model is well-established, the complex immunological mechanisms associated with filarial control and disease progression remain unclear and translation to human infections is difficult, especially since human filarial infections in rodents are limited. To overcome these obstacles, we performed adoptive immune cell transfer experiments into RAG2IL-2Rγ-deficient C57BL/6 mice. These mice lack T, B and natural killer cells and are susceptible to infection with the human filaria Loa loa. In this study, we revealed a long-term release of L. sigmodontis offspring (microfilariae) in RAG2IL-2Rγ-deficient C57BL/6 mice, which contrasts to C57BL/6 mice which normally eliminate the parasites before patency. We further showed that CD4+ T cells isolated from acute L. sigmodontis-infected C57BL/6 donor mice or mice that already cleared the infection were able to eliminate the parasite and prevent inflammation at the site of infection. In addition, the clearance of the parasites was associated with Th17 polarization of the CD4+ T cells. Consequently, adoptive transfer of immune cell subsets into RAG2IL-2Rγ-deficient C57BL/6 mice will provide an optimal platform to decipher characteristics of distinct immune cells that are crucial for the immunity against rodent and human filarial infections and moreover, might be useful for preclinical research, especially about the efficacy of macrofilaricidal drugs.

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“…When this balance is disrupted, it may lead to an inflammatory response and cause a series of nerve injuries. [ 21 ] Zerbo et al demonstrated differential expression of MCP-1, RANTES, and MIP-1α in ASD. [ 22 ] In congruence with previous reports, our findings showed that compared with healthy children, those with ASD showed significantly increased MCP-1 levels ( P < .01) and significantly decreased MIP-1α and MIP-1β levels ( P < .01).…”

Section: Discussionmentioning

confidence: 99%

“…Under normal physiological conditions, IL-8 and IP-10 are in a dynamic balance. When this balance is disrupted, it may lead to an inflammatory response and cause a series of nerve injuries [21] . Zerbo et al demonstrated differential expression of MCP-1, RANTES, and MIP-1α in ASD [22] .…”

Section: Discussionmentioning

confidence: 99%

Correlation analysis of expression of CC and CXC chemokines in children with autism spectrum disorder

Peng¹,

Peng²,

Tong³

et al. 2021

Medicine

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To investigate the relationship between the expression of CC and CXC chemokines and autism spectrum disorder (ASD). A total of 62 children with ASD (ASD group) and 60 gender- and age-matched normal children (control group) admitted to our hospital from January 2019 to January 2020 were included in the study. Monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α), macrophage inflammatory protein-1β (MIP-1β), regulated upon activation, normal T-cell expressed and secreted (RANTES), interleukin-8 (IL-8), monokine induced by interferon (IFN)-γ (MIG), and purified human interferon-γ-induced protein-10 (IP-10) were detected in the ASD group. The correlation between the above indexes and the severity of the ASD group was analyzed. Significantly increased MCP-1 levels ( P < .01) along with the markedly decreased MIP-1α and MIP-1β levels ( P < .01) were detected in the venous blood of the ASD group compared with the control group. In addition, they exhibited no significant difference (yet a downward trend) in the level of RANTES ( P > .05). Children in the ASD group showed significantly decreased IP-10 levels ( P < .01); however, they had no noticeable change (yet a decreasing trend) in the levels of IL-8 and MIG ( P > .05). MCP-1 level was positively related to the Module 1 scores of Autism Diagnostic Observation Schedule-second edition (ADOS-2), whereas the levels of Childhood Autism Rating Scale MIP-1α, MIP-1β, IL-8, IP-10, and MIG were negatively correlated with the ADOS-2 Module 1 scores ( P < .01). However, no significant correlation was found between RANTES and the ADOS-2 Module 1 scores ( P > .05). The levels of CC chemokines (MCP-1, MIP-1α, MIP-1β, and RANTES) and CXC chemokines (IL-8, IP-10, and MIG) are positively correlated with the pathogenesis of ASD. Inflammation is an important contributing factor to ASD.

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Inflammatory and regulatory CCL and CXCL chemokine and cytokine cellular responses in patients with patentMansonella perstansfilariasis

Cited by 8 publications

References 45 publications

Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models

Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models

Adoptive Transfer of Immune Cells Into RAG2IL-2Rγ-Deficient Mice During Litomosoides sigmodontis Infection: A Novel Approach to Investigate Filarial-Specific Immune Responses

Correlation analysis of expression of CC and CXC chemokines in children with autism spectrum disorder

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