Inflammatory acidic pH enhances hydrogen sulfide-induced transient receptor potential ankyrin 1 activation in RIN-14B cells

Takahashi, Kenji; Ohta, Toshio

doi:10.1002/jnr.23251

Cited by 9 publications

(11 citation statements)

References 30 publications

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“…30,31 Comparison of four groups of nerve samples Allogeneic nerve has strong immunogenicity before treatment. 32 Studies have shown that the immunogenicity of extracellular matrix components of nerves, such as laminin protein and fibronectin is weak, and even almost negligible, so the immunogenicity of allogeneic nerve mainly reflects on the cell. 33,34 Schwann cells, as the main cell components of the sciatic nerve, has the function of antigen presentation, and can produce cytokines such as IL-1 and interferon g so as to induce an immune rejection response.…”

Section: The Methods Of Preparation Of Acellular Scaffold and Its Advmentioning

confidence: 99%

The preparation and comparison of decellularized nerve scaffold of tissue engineering

Wang

Zhang

et al. 2014

J. Biomed. Mater. Res.

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To integrate tissue engineering concepts into strategies to repair spinal cord injury (SCI) has been a hotspot in recent years, and the choice of scaffolding material is crucial to tissue engineering. Recently, decellularized nerve scaffold becomes a central concern due to its peculiar superiority. In this study, the decellularized nerve scaffold was prepared with three different methods and a comparison was made to acquire an ideal scaffold materials. All sciatic nerves from Sprague-Dawley (SD) rats were randomly divided into four groups: A: normal control group, B: TritonX-100 with sodium deoxycholate group, C: TritonX-100 with enzyme group and D: freezing-thawing with enzyme group. Histology and transmission electron microscope were exploited to evaluate the effect of removing cells and immunological histological chemistry was exploited to evaluate immunogenicity. Meanwhile the mechanical properties were evaluated by mechanics index. Hematoxylin and eosin (HE) staining and electron microscopic examinations reveal that the cell components and myelin sheaths are the least in the freezing-thawing with enzyme group. Immunohistochemistry shows that the immunogenicity is lower in group B, C, and D than the control group, and the group D has the lowest immunogenicity. Mechanical testing shows that there is no significant difference after acellular processing. Sciatic nerve, cell-extracted by freezing-thawing with enzyme, could obtain the ideal scaffold materials which has no cells and myelin sheaths. In addition, the decellularized nerve scaffold has no immunogenicity and the mechanical property of normal sciatic nerve is preserved.

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Section: The Methods Of Preparation Of Acellular Scaffold and Its Advmentioning

confidence: 99%

The preparation and comparison of decellularized nerve scaffold of tissue engineering

Wang

Zhang

et al. 2014

J. Biomed. Mater. Res.

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“…The current elicited by Na 2 S 3 exhibited an outward rectifying current–voltage relationship similar to that evoked by AITC. In addition, we used RIN-14B, a rat enterochromaffin cell line that expresses TRPA1 constitutively [ 9 , 34 ]. As shown in Figure 6 C, Na 2 S 3 (10 μM) elicited [Ca 2+ ] i increases in RIN-14B cells.…”

Section: Resultsmentioning

confidence: 99%

“…The H 2 S concentration in polysulfide-containing HEPES-buffered solution was measured according to a protocol described previously [ 9 ]. In brief, Na 2 S 3 (10 μM)-containing HEPES-buffered solution (0.5 ml) was added to 10% trichloroacetic acid (0.25 ml), 1% zinc acetate (0.25 ml).…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported that H 2 S evokes neurogenic inflammation and hyperalgesia through the activation of various channels, such as transient receptor potential vanilloid 1 (TRPV1) and T-type Ca 2+ channels [4][5][6][7]. We recently reported that H 2 S stimulated a subset of mouse sensory neurons and induced pain-related behaviors [8,9]. TRPA1 and TRPV1 are nonselective cation channels expressed in nociceptive neurons and in part coexpressed in sensory neurons [10].…”

Section: Introductionmentioning

confidence: 99%

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Polysulfide Evokes Acute Pain through the Activation of Nociceptive TRPA1 in Mouse Sensory Neurons

et al. 2015

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BackgroundHydrogen sulfide (H2S) is oxidized to polysulfide. Recent reports show that this sulfur compound modulates various biological functions. We have reported that H2S is involved in inflammatory pain in mice. On the other hand, little is known about the functional role of polysulfide in sensory neurons. Here we show that polysulfide selectively stimulates nociceptive TRPA1 and evokes acute pain, using TRPA1-gene deficient mice (TRPA1(−/−)), a heterologous expression system and a TRPA1-expressing cell line.ResultsIn wild-type mouse sensory neurons, polysulfide elevated the intracellular Ca concentration ([Ca2+]i) in a dose-dependent manner. The half maximal effective concentration (EC50) of polysulfide was less than one-tenth that of H2S. The [Ca2+]i responses to polysulfide were observed in neurons responsive to TRPA1 agonist and were inhibited by blockers of TRPA1 but not of TRPV1. Polysulfide failed to evoke [Ca2+]i increases in neurons from TRPA1(−/−) mice. In RIN-14B cells, constitutively expressing rat TRPA1, polysulfide evoked [Ca2+]i increases with the same EC50 value as in sensory neurons. Heterologously expressed mouse TRPA1 was activated by polysulfide and that was suppressed by dithiothreitol. Analyses of the TRPA1 mutant channel revealed that cysteine residues located in the internal domain were related to the sensitivity to polysulfide. Intraplantar injection of polysulfide into the mouse hind paw induced acute pain and edema which were significantly less than in TRPA1(−/−) mice.ConclusionsThe present data suggest that polysulfide functions as pronociceptive substance through the activation of TRPA1 in sensory neurons. Since the potency of polysulfide is higher than parental H2S and this sulfur compound is generated under pathophysiological conditions, it is suggested that polysulfide acts as endogenous ligand for TRPA1. Therefore, TRPA1 may be a promising therapeutic target for endogenous sulfur compound-related algesic action.

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“…216 In vitro and in vivo functional studies demonstrated that H 2 S activates TRPA1. 217,218 Furthermore, acidic pH under pathophysiologic conditions potentiates the capacity of H 2 S to activate TRPA1. 217,218 Sulfur mustard SM (mustard gas, SM) has been used as a chemical WMD since World War I and was recently used in the Iran-Iraq war (1980)(1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988).…”

Section: Hydrogen Sulfidementioning

confidence: 99%

Transient receptor potential channels in pulmonary chemical injuries and as countermeasure targets

Achanta

Jordt

2020

Annals of the New York Academy of Sciences

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The lung is highly sensitive to chemical injuries caused by exposure to threat agents in industrial or transportation accidents, occupational exposures, or deliberate use as weapons of mass destruction (WMD). There are no antidotes for the majority of the chemical threat agents and toxic inhalation hazards despite their use as WMDs for more than a century. Among several putative targets, evidence for transient receptor potential (TRP) ion channels as mediators of injury by various inhalational chemical threat agents is emerging. TRP channels are expressed in the respiratory system and are essential for homeostasis. Among TRP channels, the body of literature supporting essential roles for TRPA1, TRPV1, and TRPV4 in pulmonary chemical injuries is abundant. TRP channels mediate their function through sensory neuronal and nonneuronal pathways. TRP channels play a crucial role in complex pulmonary pathophysiologic events including, but not limited to, increased intracellular calcium levels, signal transduction, recruitment of proinflammatory cells, neurogenic inflammatory pathways, cough reflex, hampered mucus clearance, disruption of the integrity of the epithelia, pulmonary edema, and fibrosis. In this review, we summarize the role of TRP channels in chemical threat agents-induced pulmonary injuries and how these channels may serve as medical countermeasure targets for broader indications.

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Inflammatory acidic pH enhances hydrogen sulfide-induced transient receptor potential ankyrin 1 activation in RIN-14B cells

Cited by 9 publications

References 30 publications

The preparation and comparison of decellularized nerve scaffold of tissue engineering

The preparation and comparison of decellularized nerve scaffold of tissue engineering

Polysulfide Evokes Acute Pain through the Activation of Nociceptive TRPA1 in Mouse Sensory Neurons

Transient receptor potential channels in pulmonary chemical injuries and as countermeasure targets

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