Inflammation-mediated matrix remodeling of extracellular matrix-mimicking biomaterials in tissue engineering and regenerative medicine

Xu, Mimi; Su, Ting; Jin, Xiaoxuan; Li, Yibao; Yao, Yao; Liu, Kaiyang; Chen, Kaiqi; Lu, Feng; He, Yunfan

doi:10.1016/j.actbio.2022.08.015

Cited by 26 publications

(21 citation statements)

References 156 publications

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“…The tissue-like structures contained new blood vessels, and the vascular structures were filled with erythrocytes ( Figure 8B and C ). The local appearance of spheroid structures ( Figure 8D and E ) or flaky tissue morphology ( Figure 8F ) suggests that heterozygous neurosecretory tissues have potential value in tissue remodeling[ 41 ]. According to the pathological sections, local immune inflammatory cells accumulated after the hybrid biofabricated tissue-like structure was transplanted in vivo , and the immune infiltration mainly occurred at places where the nanofibers were wrapped and separated ( ), which may be related to the local stimulation of the nanofibers as foreign bodies.…”

Section: Resultsmentioning

confidence: 99%

Hybrid biofabrication of neurosecretory structures as a model for neurosecretion

Tian¹,

Gu²,

Yang³

et al. 2022

IJB

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The present study aimed to combine extrusion-based three-dimensional (3D) bioprinting and polymer nanofiber electrospinning technology to fabricate tissue-like structures with neurosecretory function in vitro. Using neurosecretory cells as cell resources, sodium alginate/gelatin/fibrinogen as matrix, polylactic acid/gelatin electrospun nanofibers as diaphragm, and neurosecretory cells-loaded 3D hydrogel scaffolds were bioprinted and then covered with electrospun nanofibers layer-by-layer. The morphology was observed by scanning electron microscopy and transmission electron microscopy (TEM), and the mechanical characteristics and cytotoxicity of the hybrid biofabricated scaffold structure were evaluated. The 3D-bioprinted tissue activity, including cell death and proliferation, was verified. Western blotting and ELISA experiments were used to confirm the cell phenotype and secretory function, while animal in vivo transplantation experiments confirmed the histocompatibility, inflammatory reaction, and tissue remodeling ability of the heterozygous tissue structures. Neurosecretory structures with 3D structures were successfully prepared by hybrid biofabrication in vitro. The mechanical strength of the composite biofabricated structures was significantly higher than that of the hydrogel system (P < 0.05). The survival rate of PC12 cells in the 3D-bioprinted model was 92.849 ± 2.995%. Hematoxylin and eosin-stained pathological sections showed that the cells grew in clumps, and there was no significant difference in the expression of MAP2 and tubulin-β between 3D organoids and PC12 cells. The results of ELISA showed that the PC12 cells in 3D structures retained the ability to continuously secrete noradrenaline and met-enkephalin, and the secretory vesicles around and within the cells could be observed by TEM. In in vivo transplantation, PC12 cells gathered and grew in clusters, maintained high activity, neovascularization, and tissue remodeling in 3D structures. The neurosecretory structures were biofabricated by 3D bioprinting and nanofiber electrospinning in vitro, which had high activity and neurosecretory function. In vivo transplantation of neurosecretory structures showed active proliferation of cells and potential for tissue remodeling. Our research provides a new method for biological manufacture of neurosecretory structures in vitro, which maintains neurosecretory function and lays the foundation for the clinical application of neuroendocrine tissues.

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Section: Resultsmentioning

confidence: 99%

Hybrid biofabrication of neurosecretory structures as a model for neurosecretion

Tian¹,

Gu²,

Yang³

et al. 2022

IJB

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“…Inflammatory pathways seem to mediate extracellular matrix remodeling with neutrophils and M1 macrophages secreting a milieu of inflammatory cytokines, including interleukins, tumor necrosis factor a (TNF-a), and interferon gamma which are considered early factors that trigger the cascade of inflammation [ 42 , 43 ]. This triggers a cascade of production of several other inflammatory proteins including matrix metalloproteinases, neutrophil elastase, heparinase, and cathepsin which help modulate the extracellular matrix environment [ 8 ]. Under the influence of all these factors, the extracellular matrix undergoes a process of remodeling which results in transition from a rigid to a softer form [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Stem cells may be an intermediate factor that connects the proinflammatory environment and the histological alterations that accompany cervical insufficiency as stem cells seem to respond to inflammatory conditions in several other tissues [ 7 , 8 ]. Previous researchers have observed that stem cells are directly involved in tissue remodeling through a process that is thought to be regulated by the involvement of the extracellular matrix and specifically the macromolecules that it contains [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among these macromolecules, integrins and growth factors have been already described as mediators of the stem cell niche through an extensive crosstalk reaction with several receptors [ 7 ]. Experimental studies involving tissue engineering with extracellular matrix-mimicking biomaterials suggest that the induction of a proinflammatory state results in stem cell activation that triggers tissue proliferation and reconstruction through a process that involves the production of bioactive fragments that are termed matrikines [ 8 , 11 , 12 ]. To date, the actual mechanisms that involve tissue remodeling in cases presenting with cervical insufficiency during pregnancy remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency

Pittokopitou

Mavrogianni

Pergialiotis

et al. 2023

Cells

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The presence of stem cells has been previously described in human precancerous and malignant cervical cultures. Previous studies have shown a direct interplay of the stem cell niche, which is present in practically every tissue with the extracellular matrix. In the present study, we sought to determine the expression of stemness markers in cytological specimens collected from the ectocervix among women with cervical insufficiency during the second trimester of pregnancy and women with normal cervical length. A prospective cohort of 59 women was enrolled of whom 41 were diagnosed with cervical insufficiency. The expression of OCT-4 and NANOG was higher in the cervical insufficiency group compared to the control group (−5.03 (−6.27, −3.72) vs. −5.81 (−7.67, −5.02) p = 0.040 for OCT4) and (−7.47 (−8.78, −6.27) vs. −8.5 (−10.75, −7.14), p = 0.035 for NANOG. Differences in the DAZL gene were not significantly different (5.94 (4.82, 7.14) vs. 6.98 (5.87, 7.43) p = 0.097). Pearson correlation analysis indicated the existence of a moderate correlation of OCT-4 and Nanog with cervical length. Considering this information, the enhanced activity of stemness biomarkers among pregnant women diagnosed with cervical insufficiency may be predisposed to cervical insufficiency, and its predictive accuracy remains to be noted in larger population sizes.

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“…They preserve natural microstructures and matrix-related bioactive components and undergo continuous matrix remodeling upon transplantation. 124 Not only does it dynamically perform physical roles such as support, attach-ment, water retention, and protection but also ECM exerts an all-around influence on the cell. In the human body, ECM is usually composed of collagen, fibrin, elastin, hyaluronic acid, fibronectin, proteoglycans, growth factors, and cytokines, which can be pondered into modeling the in vivo microenvironment for cell reprogramming, differentiation, and proliferation.…”

Section: Homeostatic Construction Of Hf Organoids Via a Biomaterials ...mentioning

confidence: 99%

Innovative Approaches and Advances for Hair Follicle Regeneration

Zheng

2023

ACS Biomater. Sci. Eng.

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Pathological hair loss (also known as alopecia) and shortage of hair follicle (HF) donors have posed an urgent requirement for HF regeneration. With the revelation of mechanisms in tissue engineering, the proliferation of HFs in vitro has achieved more promising trust for the treatments of alopecia and other skin impairments. Theoretically, HF organoids have great potential to develop into native HFs and attachments such as sweat glands after transplantation. However, since the rich extracellular matrix (ECM) deficiency, the induction characteristics of skin-derived cells gradually fade away along with their trichogenic capacity after continuous cell passaging in vitro. Therefore, ECM-mimicking support is an essential prelude before HF transplantation is implemented. This review summarizes the status of providing various epidermal and dermal cells with a threedimensional (3D) scaffold to support the cell homeostasis and better mimic in vivo environments for the sake of HF regeneration. HF-relevant cells including dermal papilla cells (DPCs), hair follicle stem cells (HFSCs), and mesenchymal stem cells (MSCs) are able to be induced to form HF organoids in the vitro culture system. The niche microenvironment simulated by different forms of biomaterial scaffold can offer the cells a network of ordered growth environment to alleviate inductivity loss and promote the expression of functional proteins. The scaffolds often play the role of ECM substrates and bring about epithelial−mesenchymal interaction (EMI) through coculture to ensure the functional preservation of HF cells during in vitro passage. Functional HF organoids can be formed either before or after transplantation into the dermis layer. Here, we review and emphasize the importance of 3D culture in HF regeneration in vitro. Finally, the latest progress in treatment trials and critical analysis of the properties and benefits of different emerging biomaterials for HF regeneration along with the main challenges and prospects of HF regenerative approaches are discussed.

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Inflammation-mediated matrix remodeling of extracellular matrix-mimicking biomaterials in tissue engineering and regenerative medicine

Cited by 26 publications

References 156 publications

Hybrid biofabrication of neurosecretory structures as a model for neurosecretion

Hybrid biofabrication of neurosecretory structures as a model for neurosecretion

Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency

Innovative Approaches and Advances for Hair Follicle Regeneration

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